The regulatory mechanism of phosphatidylinositol 3-kinase by insulin in 3T3 L1 fibroblasts: Phosphorylation-independent activation of phosphatidylinositol 3-kinase

Experimental and Molecular Medicine

Volumn 30, Issue 4, 1998, Pages 263-269

  Kang, Insug ^a,b   Choi, Sang Lim ^b   Kim, Sam Soo ^a   Kim, Soo Ja ^b   Ha, Joohun ^a,b   Oh, Soo Myung ^a,b   Kim, Sung Soo ^a,b  

^a Kyung Hee University Hospital   (South Korea)

^b Kyung Hee University   (South Korea)

Author keywords

3T3 L1 fibroblast; GST p85 fusion protein; Insulin; Pl3 kinase

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; INSULIN; INSULIN RECEPTOR SUBSTRATE 1; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHOSERINE; PHOSPHOTYROSINE;

ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; ENZYME SUBUNIT; HORMONAL REGULATION; MOUSE; NONHUMAN;

ANIMALIA;

EID: 0032585552     PISSN: 12263613     EISSN: 20926413     Source Type: Journal    
DOI: 10.1038/emm.1998.39     Document Type: Article

References (26)

1. Backer, J. M., Myers Jr, M. G., Shoelson, S. E., Chin, D. J., Sun, X-J., Miralpeix, M., Hu, P., Margolis, B., Skolik, E. Y., Schlessinger, J. and White, M. F. (1992) Phosphatidylinositol 3′-kinase is activated by association with IRS-1 during insulin stimulation. EMBO J. 11: 3469-3479
2. Brown, W. J., Dewald, D. B., Emr, S. D., Plutner, H. and William, E. B. (1995) Role for phosphatidylinositol 3-kinase in the sorting and transport of newly synthesized lysosomal enzymes in mammalians cells. J. Cell Biol 130: 781-796
3. Camilli, P. D., Emr, S. D., Mcpherson, P. S. and Novick, P. (1993) Phosphoinositides as regulators in membrane traffic. Science 271: 1533-1539
4. Carpenter C. L., Auger, K. R., Duckworth, B. C., Hou, W. M., Schaffhausen, B. and Cantley, L. C. (1993) A tightly associated serine-threonine protein kinase regulate phospholinositol 3-kinase activity. Mol. Cell. Biol. 13: 1657-1665
5. Cheatham, B., Vlahos, C. J., Cheatham, L., Wang, L., Blenis, J. and Kahn, R. (1994) Phosphatidylinositol 3-kinase activation is required for insulin stimulation of pp70 S6 kinase DNA synthesis and glucose transporter translocation. Mol. Cell. Biol. 14: 4902-4911
6. Dhand, R., Hara, K., Hiles, I., Bax, B., Gout, I., Panayotou. G., Fry, M. J., Yonezawa, K., Kasuga, M. and Waterfield, M. D. (1994) Pl3-kinase: Structural and functional analysis of inter subunit interactions. EMBO J. 13: 511-521
7. Dhand, R., Hiles, I., Panayotou, G., Roche, S., Fry, M. J., Gout, L., Toffy, N. F., Truong, O., Vicendo, P., Yonezawa, K., Kasuga, M. and Waterfield M. D. (1994) Pl3-kinase is a dual specificity enzyme: autoregulation by an intrinsic protein-serine kinase activity. EMBO J. 13: 522-533
8. Divecha, N. and Irvin, R. F. (1995) Phospholipid signaling. Cell 80: 269-278
9. Escobedo, T. A., Navankasattusas, S., Kavanaugh, W. M., Milfay, D., Fried, V. A. and Williams, L. T. (1991) cDNA cloning of a novel 85 kDa protein that has SH2 domains and regulates binding of Pl3-kinase to the PDGFβ-receptor. Cell 65: 75-82
10. Hayashi, H., Nishioka, Y., Kamohara, S., Kana, F., Ishii, K., Fukui, Y., Shibasaki, F., Takenawa, T., Kido, H., Katsunuma, N. and Ebina, Y. (1993) The α-type 85 kDa subunit of Pl3-kinase is phosphorylated at tyrosine residues 368, 580, and 607 by the insulin receptor. J. Biol. Chem. 268: 7107-7117
11. Hiles, I., Otsu, D. M., Volinia, S., Fry, M. J., Gout, I., Dhand, R., Panayotou, G., Ruiz-Larrea, F., Thompson, A., Totty, N. F., Hsun, J. J., Courtneidge, S. A., Parker, P. J. and Waterfield, M. D. (1992) Phosphatidylinositol 3-kinase: Structure and expression of the 110 kDa catalytic subunit. Cell 70: 419-429
12. Hu, P., Mondino, A., Skolnik, E. Y. and Schlessinger, J. (1993) Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85. Mol. Cell. Biol. 13: 7677-7688
13. Kapeller, R., K., Prasad, V. S., Janssen, O., Hou, W., Schaffhausen, B. S., Rudd, C. E., and Cantley, L. C. (1994) Identification of two SH3-binding motifs in the regulatory sufaunit of phosphatidylinositol 3-kinase. J. Biol. Chem. 269: 1927-1933
14. Kotani, K., Yonezawa, K., Hara, K., Ueda, H., Kitamura, Y., Sakaue, H., Ando, A., Chavanieu, A., Calas, B., Grigorescu, F., Nishiyama, M., Waterfield, M. D. and Kasuga, M. (1994) Involvement of phosphoinositide 3-kinase in insulin- or IGF-1 induced membrane ruffling. EMBO J. 13: 2313-2321
15. Lam, K., Carpenter, C. L., Ruderman, N. B., Friel, J. C. and Kelly, K. L. (1994) The phosphatidylinositol 3-kinase serine kinase phosphorylates IRS-1. J. Biol. Chem. 269: 20648-20652
16. Pleiman, C. M., Hertz, W. M. and Cambier. J. C. (1994) Activation of Phosphatidylinositol-3 kinase by src-family kinase SH3 binding to the p85 subunit. Science 263: 16909-1612
17. Rahimi, N., Tremblay, E. and Elliott, B. (1996) Phophatidylinositol 3-kinase activity is required for Hepatocyte Growth Factor-induced Mitogenic signals in epithelial cells. J. Biol. Chem. 271: 24850-24855
18. Rodriguez-Viciana, P., Wame, P. H., Dhand, R., Vanhaesebroeck, B., Gout, I., Fry, M. J., Waterfield, M. D. and Downward, J. (1994) Phosphatidylinositol-3-OH kinase as a direct target of ras. Nature 370: 527-532
19. Rordorf-Nikolie, T., Van Horn, D. J., Chen, D., White, M. F. and Backer. J. M. (1995) Regulation of phosphatidyl 3′-kinase by tyrosyl phosphoproteins. J. Biol. Chem. 270: 3662-3666
20. Stephens, L., Smrcka, A., Cooke, F. T., Jackson, T. R., Sternweis, P. C. and Hawkins, P. T. (1994) A novel phosphoinositide 3-kinase activity in myeloid- derived cells is activated by G proteinβγ subunits. Cell 77: 83-93
21. Stoyanov, B., Volinia, S., Hanck, T., Rubio, I., Loubtchenkov, M., Malek, D., Stoyanova, S., Vanhaesebroeck, B., Dhand, R., Nurnberg, B., Gierschik., G., Seedorf, K., Nurnberg, B., Gierschik, P., Seedorf, K., Hsuan, J. J., Waterfield, M. D. and Wetzker, R. (1995) Cloning and characterization of a G protein-activated human phosphoinositide 3-kinase. Science 269: 690-693
22. 3 production in rat skeletal muscle in vivo. J. Biol. Chem. 272: 5477-5481
23. Whitman, M., Kaplan, D. R., Schaffhausen, B., Cantley, L. C. and Roberts, T. M. (1985) Association of phophatidylinositol kinase activity with polyoma middle T competent for transformation. Nature 315: 239-242
24. Whitman, M., Downes, C. P., Keller, M. and Cantley, L. C. (1988) Type I phosphatidylinositol- kinase makes a novel inositol phospholipid, phosphatidylinositol-3 -phosphate. Nature 332: 644-646
25. Woscholski, R., Dhand, R., Fry, M. J., Waterfield, M. D. and Parker, P. J. (1994) Biochemical characterization of the free catalytic p110α and the complexed heterodimeric p110α·p85α forms of the mammalian phosphatidylinositol 3-kinase. J. Biol. Chem. 269: 25067-25072
26. Yu, J., Zhang, Y., Mcilroy, J., Rordorf-Nikolie, T., Orr, G. E. and Backer. J. M. (1998) Regulation of the p85/p110 phosphatidylinositol 3-kinase: Stabilization and inhibition of the p110α catalytic subunit by the p85 regulatory subunit. Mol. Cell. Biol. 18: 1379-1387