A new high resolution radioimager for the quantitative analysis of radiolabelled molecules in tissue section

Journal of Neuroscience Methods

Volumn 86, Issue 1, 1998, Pages 1-5

  Laniece, P ^a   Charon, Y ^a   Cardona, A ^b   Pinot, L ^a   Maitrejean, S ^c   Mastrippolito, R ^a   Sandkamp, B ^c   Valentin, L ^a  

^a Orsay Campus   (France)

^b CNRS   (France)

^c PSL RESEARCH UNIVERSITY   (France)

Author keywords

Autoradiography; In situ hybridization; Intensified CCD; Quantification; Radioimaging; Receptor binding

Indexed keywords

CARBON 14; IODINE 125; PHOSPHORUS 32; RADIOISOTOPE; SULFUR 35; TRITIUM;

ARTICLE; AUTORADIOGRAPHY; IMAGE INTENSIFIER; IN SITU HYBRIDIZATION; ISOTOPE LABELING; OPTICAL RESOLUTION; PRIORITY JOURNAL; RECEPTOR BINDING; TISSUE SECTION;

ANIMALS; AUTORADIOGRAPHY; BETA RAYS; IN SITU HYBRIDIZATION; INTESTINES; KIDNEY; RATS; TRITIUM;

EID: 0032585494     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(98)00128-9     Document Type: Article

References (18)

1. Charpak G, Dominik W, Zaganidis N Optical imaging of the spatial distribution of beta particles emerging from flat surfaces. Proc Natl Acad Sci USA. 86:1989;1741-1745.
2. Charon Y, Gaillard JM, Laniece P et al. Imageur haute resolution a teas niveau de lumiere. Patent number 90 10593, 1990.
3. Charon Y, Laniece P, Bendali M et al. A self triggered intensified CCD (STIC). Nucl Instrum Methods. A310:1991;379-384.
4. Charon Y, Lanièce P, Mastrippolito R, Siebert R, Tricoire H, Valentin L Quantitative nuclear imaging in biology. Ann Phys Fr. 22-6:1997;707-770.
5. Crumeyrolle-Arias M, Latouche J et al. In situ characterization of GnRH receptors: use of two radioimagers and comparison with quantitative autoradiography. J Receptor Res. 14:1994;251-265.
6. Crumeyrolle-Arias M, Jafarian M et al. Radioimagers as alternative to film autoradiography for in situ quantitative analysis of receptor binding and pharmacological studies. Histochem J. 28:1996;801-809.
7. Ito T, Suzuki T, Lim DK, Wellman SE, Ho IK A novel quantitative receptor autoradiography and in situ hybridization histochemistry technique using storage phosphor screen imaging. J Neurosci Methods. 59:1995;265-271.
8. Johnston RF, Pickett SC, Barker DL Autoradiography using storage phosphor technology. Electrophoresis. 11:1990;355-360.
9. Kuhar MJ, Unnerstall JR Quantitative receptor mapping by autoradiography: some current technical problems. TINS. 8:1985;49-53.
10. Kuhar MJ, Unnerstall JR Receptor autoradiography. Yamamura HI, Enna SJ, Kuhar MJ Methods in Neurotransmitter Receptor Analysis. 1990;177-218 Raven Press, New York.
11. Laniece P, Charon Y, Dumas S et al. HRRI: a high resolution radioimager for fast direct quantification in in situ hybridization experiments. Bio Techniques. 17:1994;338-345.
12. Laniece P, Lehir H, Bodeau-Pean S et al. Evidence of a novel mRNA rat tyrosine hydroxylase generated by altemative splicing. J Neurochem. 66:1996;1819-1825.
13. Marquette C, Van Dam AM, Ban E et al. Rat interleukine 1 β binding sites in the rat hypothalamus and pituitary gland. Neuroendocrinology. 62:1995;362-369.
14. Palacios JM, Niehoff DL, Kuhar MJ Receptor autoradiography with tritium-sensitive film: potential for computerized densitometry. Neurosci Lett. 25:1981;101-105.
15. Palacios JM, Mengod G, Vilaro MT, Ramm P Recent trends in receptor analysis techniques and instrumentation. J Chem Neuroanat. 4:1991;343-353.
16. Ramm P Advanced image analysis systems in cell, molecular and neurobiology applications. J Neurosci Methods. 54:1994;131-149.
17. Rougeot C, Vienet R, Cardona A, Le Doledec L, Grognet JM, Rougeon F Targets for SMR1pentapeptide suggest a link between the circulating peptide and mineral transport. Am J Physiol. 273:1997;R1309-R1320.
18. 3H]vasopressin binding sites in the brain of jerboa (jaculus orientalis) by an high resolution β-radio imager. J Neurosci Methods. 49:1993;231-240.