Plasmid pIP501 encoded transcriptional repressor CopR binds to its target DNA as a dimer

Journal of Molecular Biology

Volumn 283, Issue 3, 1998, Pages 595-603

  Steinmetzer, Katrin ^a   Behlke, Joachim ^b   Brantl, Sabine ^a  

^a Inst F Molekularbiologie F   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Binding stoichiometry; CopR; DNA protein interaction; Plasmid replication control; Transcriptional repressor

Indexed keywords

DNA BINDING PROTEIN;

ARTICLE; BACILLUS SUBTILIS; DIMERIZATION; DISSOCIATION CONSTANT; DNA BINDING; IMMUNOBLOTTING; NONHUMAN; PLASMID; PRIORITY JOURNAL; REPRESSOR GENE; TRANSCRIPTION REGULATION;

BACILLUS SUBTILIS; POSIBACTERIA;

EID: 0032582825     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.2122     Document Type: Article

References (31)

1. Abril A.M., Salas M., Andreu J.M., Hermoso J.M., Rivas G. Phage Φ29 protein p6 is in a monomer-dimer equilibrium that shifts to higher association states at the millimolar concentrations found in vivo. Biochemistry. 36:1997;11901-11908
2. Backman K., Ptashne M., Gilbert W. Construction of plasmids carrying the cI gene of bacteriophage λ Proc. Natl Acad. Sci. USA. 73:1976;4174-4178
3. Bain D.L., Ackers G. Self-association and DNA binding of lambda cI repressor N-terminal domains reveal linkage between sequence-specific binding and the C-terminal cooperativity domain. Biochemistry. 33:1994;14679-14689
4. Behlke J., Ristau O., Schönfeld H.-J. Nucleotide-dependent complex formation between the Escherichia coli chaperonins GroEL and GroES studied under equilibrium conditions. Biochemistry. 36:1997;5149-5156
5. Brantl S. The CopR protein of plasmid pIP501 acts as transcriptional repressor at the essential repR promoter. Mol. Microbiol. 14:1994;473-483
6. Brantl S., Behnke D. Copy number control of the streptococcal plasmid pIP501 occurs at three levels. Nucl. Acids Res. 20:1992;395-400
7. Brantl S., Wagner E.G.H. Antisense RNA-mediated transcriptional attenuation occurs faster than stable antisense/target RNA pairing an in vitro study of plasmid pIP501. EMBO J. 13:1994;3599-3607
8. Brantl S., Wagner E.G.H. An unusually long-lived antisense RNA in plasmid copy number control in vivo RNAs encoded by the streptococcal plasmid pIP501. J. Mol. Biol. 255:1996;275-288
9. Brantl S., Wagner E.G.H. Dual function of the copR gene product of plasmid pIP501. J. Bacteriol. 179:1997;7016-7024
10. Brantl S., Behnke D., Alonso J.C. Molecular analysis of the replication region of the conjugative Streptococcus agalactiae plasmid pIP501 in Bacillus subtilis. Comparison with plasmids pAMβ1 and pSM19035. Nucl. Acids Res. 18:1990;4783-4790
11. Brantl S., Birch-Hirschfeld E., Behnke D. RepR protein expression on plasmid pIP501 is controlled by an antisense RNA-mediated transcription attenuation mechanism. J. Bacteriol. 175:1993;4052-4061
12. Brenner M., Tomizawa J. Quantitation of ColE1-encoded replication elements. Proc. Natl Acad. Sci. USA. 88:1991;405-409
13. Ceglowski P., Lurz R., Alonso J.C. Functional analysis of pSM19035 derived replicons in Bacillus subtilis. FEMS Microbiol. Letters. 109:1993;2-3
14. Chakerian A.E., Matthews K.S. Effect of lac repressor oligomerization on regulatory outcome. Mol. Microbiol. 6:1992;963-968
15. del Solar G.H., Perez-Martin J., Espinosa M. Plasmid pLS1-encoded RepA protein regulates transcription from repAB promoter by binding to a sequence containing a 13-base pair symmetric element. J. Biol. Chem. 265:1990;12569-12575
16. Harrison S.C. A structural taxonomy of DNA-binding domains. Nature. 353:1991;715-719
17. Hope I.A., Struhl K. GCN4, a eukaryotic transcriptional activator protein, binds as a dimer to target DNA. EMBO J. 6:1987;2781-2784
18. Horodniceanu T., Bouanchaud D.H., Bieth G., Chabbert Y.A. R-plasmids in Streptococcus agalactiae (group B). Antimicrob. Agents Chemother. 10:1976;795-801
19. Jana R., Hazbun T.R., Mollah A.K., Mossing M.C. A folded monomeric intermediate in the formation of lambda Cro dimer-DNA complexes. J. Mol. Biol. 273:1997;402-416
20. Kelley R.L., Yanofsky C. trp aporepressor production is controlled by autogenous regulation and inefficient translation. Proc. Natl Acad. Sci. USA. 79:1982;3120-3124
21. Le Chatelier E., Ehrlich S.D., Janniére L. The pAMβ1 Cop repressor regulates plasmid copy number by controlling transcription of the repE gene. Mol. Microbiol. 14:1994;463-471
22. LeTilly V., Royer C.A. Fluorescence anisotropy assays implicate protein-protein interactions in regulating trp repressor DNA binding. Biochemistry. 32:1993;7753-7758
23. Light J., Molin S. The sites of action of the two copy number control functions of plasmid R1. Mol. Gen. Genet. 187:1982;486-493
24. Riise E., Molin S. Purification and characterization of the CopB replication control protein, and precise mapping of its target site in the R1 plasmid. Plasmid. 15:1986;163-171
25. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
26. Sanger F., Nicklen E.F., Coulson A.R. DNA sequencing with chain terminating inhibitors. Proc. Natl Acad. Sci. USA. 74:1977;5463-5467
27. Steinmetzer K., Brantl S. Plasmid pIP501 encoded transcriptional repressor CopR binds asymmetrically at two consecutive major grooves of the DNA. J. Mol. Biol. 269:1997;684-693
28. Swinfield T.J., Oultram J.D., Thompson D.E., Brehm J.K., Minton N.P. Physical characterisation of the replication region of the Streptococcus faecalis plasmid pAMβ1. Gene. 87:1990;79-90
29. Valentin-Hansen P., Svenningsen B.A., Munch-Petersen A., Hammer-Jespersen K. Regulation of the deo operon in Escherichia coli. Mol. Gen. Genet. 159:1978;191-202
30. Watnick P.I., Eto T., Takahashi H., Calderwood S.B. Purification of Vibrio cholerae Fur and estimation of its intracellular abundance by antibody sandwich enzyme-linked immunosorbent assay. J. Bacteriol. 179:1997;243-247
31. Willins D.A., Ryan C.W., Platko J.V., Calvo J.M. Characterization of Lrp, an Escherichia coli regulatory protein that mediates a global response to leucine. J. Biol. Chem. 266:1991;10768-10774