Formation of the gap junction intercellular channel requires a 30°rotation for interdigitating two apposing connexons

Journal of Molecular Biology

Volumn 277, Issue 2, 1998, Pages 171-177

  Perkins, Guy A ^a   Goodenough, Daniel A ^b   Sosinsky, Gina E ^c  

^a SAN DIEGO STATE UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Computational docking; Connexon structure; Electron microscopy and image processing; Molecular recognition; Three dimensional reconstruction

Indexed keywords

GAP JUNCTION PROTEIN;

ARTICLE; CELL COMMUNICATION; COMPUTER MODEL; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; GAP JUNCTION; HYDROGEN BOND; HYDROPHOBICITY; IMAGE PROCESSING; MEMBRANE CHANNEL; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

EID: 0032571358     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.1580     Document Type: Article

References (28)

1. Baker T.S., Caspar D.L.D., Hollingshead C.J., Goodenough D.A. Gap junction structures. IV. Asymmetric features revealed by low-irradiation microscopy. J. Cell Biol. 96:1983;204-216
2. Baker T.S., Sosinsky G.E., Caspar D.L.D., Gall C., Goodenough D.A. Gap junction structures. VII. Analysis of connexon images obtained with cationic and anionic negative stains. J. Mol. Biol. 184:1985;81-98
3. Dahl G. Where are the gates in gap junction channels? Clin. Expt. Pharmacol. Physiol. 23:1996;1047-1052
4. Dahl G., Werner R., Levine E., Rabadan-Diehl C. Mutational analysis of gap junction formation. Biophys. J. 62:1992;172-180
5. Foote C.I., Zhu X., Zhou L., Nicholson B.J. Pattern of disulfide linkages in the extracellular loop regions of Cx32 suggest a model of the docking interfaces of gap junctions. J. Cell Biol. 1998;. In the press
6. Ghoshroy S., Goodenough D.A., Sosinsky G.E. Preparation, characterization, and structure of half gap junctional layers split with urea and EGTA. J. Membr. Biol. 146:1995;15-28
7. Gogol E., Unwin N. Organization of connexons in isolated rat liver gap junctions. Biophys. J. 54:1988;105-112
8. Goodenough D.A., Stoeckenius W. Isolation of mouse hepatocyte gap junctions. Prelimary chemical characterization and X-ray diffraction. J. Cell Biol. 54:1972;646-656
9. Goodenough D.A., Paul D.L., Jesaitis L. Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions. J. Cell Biol. 107:1988;1817-1824
10. Goodenough D.A., Goliger J.A., Paul D.L. Connexins, connexons, and intercellular communication. Annu. Rev. Biochem. 65:1996;475-502
11. Haubrich S., Schwarz H.J., Bukauskas F., Lichtenberg-Frate H., Traub O., Weingart R., Willecke K. Incompatibility of connexin 40 and 43 hemichannels in gap junctions between mammalian cells is determined by intracellular domains. Mol. Biol. Cell. 7:1996;1995-2006
12. Hoh J., Sosinsky G.E., Revel J.-P., Hansma P.K. Structure of the extracellular surface of the gap junction by atomic force microscopy. Biophys. J. 65:1993;149-163
13. Jiang J.X., Goodenough D.A. Heteromeric connexons in lens gap junction channels. Proc. Natl Acad. Sci. USA. 3:1996;1287-1291
14. Kistler J., Goldie K., Donaldson P., Engel A. Reconstitution of native-type noncrystalline lens fiber gap junctions from isolated hemichannels. J. Cell Biol. 126:1994;1047-1058
15. Konig N., Zampighi G.A. Purification of bovine lens cell-to-cell channels composed of connexin44 and connexin50. J. Cell Sci. 108:1995;3091-3098
16. Makowski L., Caspar D.L.D., Goodenough D.A., Phillips W.C. Gap junction structures. III. The effect of variations in isolation procedures. Biophys. J. 37:1982;189-191
17. McRee D. Practical Protein Crystallography. 1993;Academic Press Ltd, San Diego, U.S.A
18. Musil L.S., Goodenough D.A. Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques. J. Cell Biol. 115:1991;1357-1374
19. Perkins G.A., Goodenough D.A., Sosinsky G.E. Three-dimensional structure of the gap junction connexon. Biophys. J. 72:1997;533-544
20. Sikewar S.S., Unwin N. Three-dimensional structure of gap junctions in fragmented plasma membranes from rat liver. Biophys. J. 54:1988;113-119
21. Song L., Hobaugh H.R., Shustak C., Cheley S., Bayley H., Gouaux J.E. Structure of the staphylococcal α-hemolysin, a heptameric transmembrane pore. Science. 274:1996;1859-1866
22. Sosinsky G.E. Image analysis of gap junction structures. Electron Microsc. Rev. 3:1992;59-76
23. Sosinsky G.E. Molecular organization of gap junction membrane channels. J. Bioenerg. Biomembr. 28:1996;297-310
24. Stauffer K.A., Kumar N.M., Gilula N.B., Unwin N. Isolation and purification of gap junction channels. J. Cell Biol. 115:1991;141-150
25. Unger V.M., Kumar N.M., Gilula N.B., Yeager M. Projection structure of a gap junction membrane channel at 7 Å resolution. Nature Struct. Biol. 4:1997;39-43
26. Unwin P.N.T., Ennis P.D. Two configurations of a channel-forming membrane protein. Nature. 307:1984;609-613
27. Unwin P.N.T., Zampighi G. Structure of the junction between communicating cells. Nature. 283:1980;545-549
28. White T.W., Bruzzone R., Wolfram S., Paul D.L., Goodenough D.A. Selective interactions among multiple connexin proteins expressed in the vertebrate lens the second extracellular domain is a determinant of compatibility between connexins. J. Cell Biol. 125:1994;879-892