Changes in auxin response from mutations in an AUX/IAA gene

Science

Volumn 279, Issue 5355, 1998, Pages 1371-1373

  Rouse, Dean ^a   Mackay, Pamela ^a   Stirnberg, Petra ^a   Estelle, Mark ^b   Leyser, Ottoline ^a  

^a UNIVERSITY OF YORK   (United Kingdom)

^b INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARTICLE; GENE FUNCTION; GENE MUTATION; GENETIC TRANSCRIPTION; PLANT; PLANT GROWTH; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CHROMOSOME MAPPING; CLONING, MOLECULAR; GENES, PLANT; INDOLEACETIC ACIDS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR PROTEINS; PHENOTYPE; PLANT PROTEINS; POINT MUTATION; RNA SPLICING; SIGNAL TRANSDUCTION; SUPPRESSION, GENETIC;

ARABIDOPSIS; EMBRYOPHYTA;

EID: 0032570689     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5355.1371     Document Type: Article

References (24)

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15. 32P-labeled probes. BAC ends were isolated by inverse PCR as described (http://194.94.225.1/private_workgroups/pg_101/bac_ end_prep.html#ipcr) or by internal deletion by restriction enzyme digestion and religation.
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19. Four primer pairs were used to amplify overlapping fragments of the IAA17/AXR3 gene from mutant allele DNA: 5′-ATCTTCCTCATCACCTTCCC-3′ and 5′-GGACAAAGCATTAGAAAGC-3′, 5′-TGTGACATCACGTCAAAG-3′ and 5′-AAGGGAGAAGAAGAAGACG-3′, 5′-GCACCATAAAAGAAAACCAC-3′ and 5′-CAAGTTATGCGGTTGAGG-3′, and 5′-AGCTTACAAAAGAGATTTGC-3′ and 5′-TTTCACTTTCAAGACGAACC-3′. RNA was extracted from 3-day-old axr3-1R4 and axr3-1R5 etiolated seedlings as described [J. Logemann et al., Anal. Biochem. 163, 16 (1987)]. Complementary DNA synthesis was performed with an oligo(dT) primer and reverse transcriptase (Superscript, Gibco-BRL). A region including the boundaries of the second and third introns was amplified with ′-CATACCGGAAGAACGTGATG-3′ and 5′-CCAATGGCATCCGATCCTTTC-3′ as primers. PCR products were purified with a QIAGEN QIA quick PCR purification kit and sequenced (12) with the use of the PCR primers and additional internal primers. GenBank accession numbers for the semidominant and intragenic revenant mutations are AF040631 and AF040632, respectively.
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23. The plants were grown on vertically oriented petri dishes under a 16-hour-light, 8-hour-dark cycle at 22°C on antithymocyte serum medium (17) solidified with 1% agar.
24. We thank J. R. Ecker and T. Altman for sharing clones and hybridization results, S. Theologis for providing unpublished data, horticultural technicians at the University of York for plant care, the Arabidopsis Biological Resource Center Ohio and members of the Arabidopsis community for supplying seeds and clones, S. Freeman and W. Cooper for contributions to the work, and S. Day for critical reading of the manuscript. Supported by the Biotechnology and Biological Sciences Research Council of the United Kingdom.