ARF1, a transcription factor that binds to auxin response elements

Science

Volumn 276, Issue 5320, 1997, Pages 1865-1868

  Ulmasov, Tim ^a   Hagen, Gretchen ^a   Guilfoyle, Tom J ^a  

^a University of Missouri   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUXIN; PHYTOHORMONE; TRANSCRIPTION FACTOR;

AMINO ACID COMPOSITION; AMINO TERMINAL SEQUENCE; ARABIDOPSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; MOLECULAR CLONING; NONHUMAN; PHYTOCHEMISTRY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE SEQUENCE; BINDING SITES; CLONING, MOLECULAR; DNA, PLANT; DNA-BINDING PROTEINS; GENES, PLANT; INDOLEACETIC ACIDS; MOLECULAR SEQUENCE DATA; MUTATION; PLANT PROTEINS; PROMOTER REGIONS (GENETICS); REPETITIVE SEQUENCES, NUCLEIC ACID; TRANSCRIPTION FACTORS;

EID: 0030796237     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5320.1865     Document Type: Article

References (23)

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6. 8 transformants, 500 colonies grew on - His plates containing 3-AT, and these were screened for lacZ activity. LacZ-positive colonies (212) were selected, and library plasmids were isolated. Sizes of the cDNA inserts were determined by polymerase chain reaction (PCR) with primers that flanked the cDNA inserts. Plasmids harboring different-sized inserts were rescued by transformation into E. coli and retransformed into the Y4271::P3(4×) strain. Clones that restored lacZ activity were sequenced, and five clones encoding ARF1 were recovered.
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15. 2-terminal regions of ARF1-BP cDNA were isolated by PCR.
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20. Abbreviations for the amino acid residues are: A, Ala; C, Cys; D. Asp; E, Glu; F, Phe; G, Gly; H, His; I, IIe; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W. Trp; and Y, Tyr.
21. Histidine-tagged ARF1 was expressed and purified from E. coli, full-length and truncated forms of ARF1 were synthesized in vitro in FlexiRabbit reticulocyte lysates (Promega), and gel-shift assays were done as described (76).
22. The IAA24 ORF was isolated from the MATCHMAKER cDNA library by PCR. IAA24 protein was produced by in vitro translation.
23. Supported by NSF grants IBN 9303956 and MCB 9604208. We thank W. Yu and X. Feng for technical assistance.