Activation of the protein kinase p38 in the spindle assembly checkpoint and mitotic arrest

Science

Volumn 280, Issue 5363, 1998, Pages 599-661

  Takenaka, Katsuya ^a   Moriguchi, Tetsuo ^a   Nishida, Eisuke ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANISOMYCIN; ARSENIC TRIOXIDE; IMMUNOGLOBULIN G; MITOGEN ACTIVATED PROTEIN KINASE; NOCODAZOLE; SYNAPTOPHYSIN;

ANIMAL CELL; ARTICLE; CELL CYCLE PHASE; EMBRYO DEVELOPMENT; ENZYME ACTIVATION; MITOSIS INHIBITION; MITOSIS SPINDLE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; XENOPUS;

3T3 CELLS; ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; ENZYME ACTIVATION; ENZYME INHIBITORS; G1 PHASE; HELA CELLS; HUMANS; IMIDAZOLES; IMMEDIATE-EARLY PROTEINS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; MAP KINASE KINASE 6; MATURATION-PROMOTING FACTOR; MICE; MITOGEN-ACTIVATED PROTEIN KINASES; MITOSIS; MITOTIC SPINDLE APPARATUS; NOCODAZOLE; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PROTEIN-TYROSINE-PHOSPHATASE; PYRIDINES; RECOMBINANT PROTEINS; S PHASE; XENOPUS; XENOPUS PROTEINS;

EID: 0032562716     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5363.599     Document Type: Article

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39. 0. Fresh DMEM supplemented with 10% CS was then added to induce the cells to reenter the cell cycle. After 18 hours, nocodazole (0.4 μg/ml) was added before cells entered M phase. Mitotic cells were collected 4 hours later by mechanical shake-off, washed with phosphate-buffered saline (PBS), and lysed in 50 mM Hepes (pH 7.4), 100 mM NaCl, 1 mM EGTA, 20 mM NaF, 20 mM sodium pyrophosphate, 1 mM sodium vanadate, 1% aprotinin, 1 mM phenylmethylsulfonyl fluoride, and 1% Triton X-100. Cells remaining on flasks were scraped and lysed in the same buffer.
40. 2 for 10 min at 30°C. The reactions were terminated by the addition of SDS - polyacrylamide gel electrophoresis (PAGE) sample buffer. After boiling, the samples were subjected to SDS-PAGE and autoradiography.
41. 2 for 30 min at 30°C. After electrophoresis, radioactivity was detected by autoradiography or analyzed with an image analyzer (BAS2000, Fuji Photo Film). Similar results were obtained with an antibody to recombinant p38 that we produced.
42. 2 for 30 min at 30°C. JNK was immuno-precipitated with an antibocty to a COOH-terminal peptide of JNK (Santa Cruz) and assayed as described [T. Moriguchi, H. Kawasaki, S. Matsuda, Y. Gotoh, E. Nishida, J. Biol. Chem. 270, 12969 (1995)].
43. 2 for 30 min at 30°C. JNK was immuno-precipitated with an antibocty to a COOH-terminal peptide of JNK (Santa Cruz) and assayed as described [T. Moriguchi, H. Kawasaki, S. Matsuda, Y. Gotoh, E. Nishida, J. Biol. Chem. 270, 12969 (1995)].
44. Xenopus egg extracts were prepared as described [A. W. Murray, Methods Cell Biol. 36, 573 (1991)]. Recombinant GST (gluthathione S-transferase) - p38 and histidine-tagged MKK6 proteins were added to the extracts at a final concentration of 0.1 mg/ml each.
45. Recombinant GST-p38 protein was incubated with or without recombinant MKK6 protein (17). Ste11AN was expressed in E. coli as described (6). Recombinant proteins were then added to the cell cycle extracts (final concentration, 0.1 mg/ml).
46. Samples (10 nl) were microinjected into one blastomere of the two-cell embryo as described (4).
47. We thank Y. Grotoh and M. Fukuda for helpful discussions and N. Masuyama for assistance in the microinjection experiments. K.T. and T.M. are Research Fellows of the Japan Society for the Promotion of Science. Supported by Grants-in-Aid from the Ministry of Education, Science, and Culture of Japan (E.N.).