Is a hydrophobic amino acid required to maintain the reactive V conformation of thiamin at the active center of thiamin diphosphate- requiring enzymes? Experimental and computational studies of isoleucine 415 of yeast pyruvate decarboxylase

Biochemistry

Volumn 37, Issue 38, 1998, Pages 13379-13391

  Guo, Fusheng ^a   Zhang, Deqi ^a   Kahyaoglu, Ara ^a   Farid, Ramy S ^a   Jordan, Frank ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ISOLEUCINE; PYRUVATE DECARBOXYLASE; THIAMINE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME STRUCTURE; HUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

AMINO ACID SUBSTITUTION; BINDING SITES; BINDING, COMPETITIVE; COMPUTATIONAL BIOLOGY; ISOLEUCINE; KINETICS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PYRUVATE DECARBOXYLASE; SUBSTRATE SPECIFICITY; THIAMINE PYROPHOSPHATE;

EID: 0032558425     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9807097     Document Type: Article

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