Translocation of phosducin in living neuroblastoma x glioma hybrid cells (NG 108-15) monitored by red-shifted green fluorescent protein

Brain Research

Volumn 790, Issue 1-2, 1998, Pages 347-356

  Schulz, Rüdiger ^a   Schulz, Karin ^a   Wehmeyer, Andrea ^a   Murphy, John ^b  

^a UNIVERSITY OF MUNICH   (Germany)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Confocal microscopy; Fusion protein; G proteins; Green fluorescence protein; Phosducin; Prostaglandin e1; Protein trafficking

Indexed keywords

GREEN FLUORESCENT PROTEIN; PHOSDUCIN;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; GLIOMA; HYBRID CELL; NEUROBLASTOMA; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TARGETING;

ALPROSTADIL; ANIMALS; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CELL MEMBRANE; COLOR; CYCLIC AMP; EYE PROTEINS; FLOW CYTOMETRY; GENE EXPRESSION; GLIOMA; GREEN FLUORESCENT PROTEINS; GTP-BINDING PROTEIN REGULATORS; GTP-BINDING PROTEINS; HYBRID CELLS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MICE; MICROSCOPY, CONFOCAL; NEUROBLASTOMA; PHOSPHOPROTEINS; PROTEIN KINASES; RATS; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 0032550186     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(98)00114-0     Document Type: Article

References (44)

1. H. Ammer, R. Schulz, Coupling of prostaglandin E1 receptors to the stimulatory GTP-binding protein Gs is enhanced in neuroblastoma X glioma (NG 108-15) hybrid cells chronically exposed to an opioid, Mol. Pharm. 43 (1993) 556-563.
2. H. Ammer, R. Schulz, Chronic exposure of NG 108-15 cells to inhibitory acting drugs reduces stimulatory prostaglandin E1 receptor number, Eur. J. Pharmacol. 302 (1996) 199-205.
3. L.S. Barak, S.S.G. Ferguson, J. Zhang, M.G. Caron, A β-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation, J. Biol. Chem. 272 (1997) 27497-27500.
4. L.S. Barak, S.S.G. Ferguson, J. Zhang, C. Martenson, T. Meyer, M.G. Caron, Internal trafficking and surface mobility of a functional intact β2-adrenergic receptor-green fluorescent protein conjugate, Mol. Pharm. 51 (1997) 177-184.
5. P.H. Bauer, S. Müller, M. Puzicha, S. Pippig, B. Obermaier, E.J.M. Helmreich, M.J. Lohse, Phosducin is a protein kinase A-regulated G-protein regulator, Nature 358 (1992) 73-75.
6. I. Boekhoff, K. Touhara, S. Danner, J. Inglese, M.J. Lohse, H. Breer, R.J. Lefkowitz, Phosducin, potential role in modulation of olfactory signaling, J. Biol. Chem. 272 (1997) 4606-4612.
7. S.G. Boxer, Another green revolution, Nature 383 (1996) 484-485.
8. M. Chalfie, Y. Tu, G. Euskirchen, W.W. Ward, D.C. Prasher, Green fluorescent protein as a marker for gene expression, Science 263 (1994) 802-805.
9. T.T. Chuang, M. Sallese, G. Ambrosini, G. Parruti, A. de Blasi, High expression of beta-adrenergic receptor kinase in human peripheral blood leukocytes: Isoproterenol and platelet activating factor can induce kinase translocation, J. Biol. Chem. 267 (1992) 6886-6892.
10. B.P. Cormack, R. Valdivia, S. Falkow, FACS-optimized mutants of the green fluorescent protein (GFP), Gene 173 (1996) 33-38.
11. Y. Daaka, J.A. Pitcher, M. Richardson, R.H. Stoffel, J.D. Robishaw, R.J. Lefkowitz, Receptor and Gβγ isoform-specific interaction with G protein-coupled receptor kinases, Proc. Natl. Acad. Sci. USA 94 (1997) 2180-2185.
12. S. Danner, M.J. Lohse, Phosducin is a ubiquitous G-protein regulator, Proc. Natl. Acad. Sci. USA 93 (1996) 10145-10150.
13. S.P. Dobson, C. Livingstone, G.W. Gould, J.M. Tavare, Dynamics of insulin-stimulated translocation of GLUT4 in single living cells visualized using green fluorescent protein, FEBS Lett. 393 (1996) 179-184.
14. R. Gaudet, A. Bohm, P.B. Sigler, Crystal structure at 2.4 Å resolution of the complex of transducin βγ and its regulator, phosducin, Cell 87 (1997) 577-588.
15. A.G. Gilman, G proteins: transducers of receptor-generated signals, Ann. Rev. Biochem. 56 (1987) 615-649.
16. K. Haga, T. Haga, Activation by G protein bg subunits of agonist- or light-dependent phosphorylation of muscarinic acetylcholine receptors and rhodopsin, J. Biol. Chem. 267 (1992) 2222-2227.
17. T. Haga, K. Haga, K. Kameyama, G protein-coupled receptor kinases, J. Neurochem. 63 (1994) 400-412.
18. B.E. Hawes, K. Touhara, H. Kurose, R.J. Lefkowitz, J. Inglese, Determination of the beta gamma-binding domain of phosducin. A regulatable modulator of G beta gamma signaling, J. Biol. Chem. 269 (1994) 29825-29830.
19. M. Heckman, P.H. Bauer, P. Söhlemann, M.J. Lohse, Phosducin inhibits receptor phosphorylation by the β-adrenergic receptor kinase in a PKA-regulated manner, FEBS Lett. 343 (1994) 120-124.
20. J. Inglese, W.J. Koch, M.G. Caron, R.J. Lefkowitz, Isoprenylation in regulation of signal transduction by G-protein-coupled receptor kinases, Nature 359 (1992) 147-150.
21. M. Johansson, S. Brismar, A. Karlsson, Human deoxycytidine kinase is located in the cell nucleus, Proc. Natl. Acad. Sci. USA 94 (1997) 11941-11945.
22. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 621-628.
23. J. Lang, R. Schulz, Chronic opiate receptor activation in vivo alters the level of G-protein subunits in the guinea-pig myenteric plexus, Neuroscience 32 (1989) 503-510.
24. R.H. Lee, B.S. Lieberman, R.N. Lolley, A novel complex from bovine visual cells of a 33,000-dalton phosphoprotein with β- and γ-transducin: purification and subunit structure, Biochemistry 26 (1987) 3983-3990.
25. R.H. Lee, T.D. Ting, B.S. Liebermann, D.E. Tobias, R.N. Lolley, Y.K. Ho, Regulation of retinal cGMP cascade by phosducin in bovine rod photoreceptor cells, J. Biol. Chem. 267 (1992) 25104-25112.
26. R.L. Lefkowitz, G protein-coupled receptor kinases, Cell 74 (1993) 409-412.
27. O.H. Lowry, N.J. Rosenbrough, A.L. Farr, R.J. Randall, Protein measurement with the folin phenol reagent, J. Biol. Chem. 193 (1951) 265-275.
28. L. Lybarger, D. Dempsey, K.J. Franek, R. Chervenak, Rapid generation and flow cytometric analysis of stable GFP-expressing cells, Cytometry 25 (1996) 211-220.
29. T. Misteli, D.L. Spector, Applications of the green fluorescent protein in cell biology, Nat. Biotech. 15 (1997) 961-964.
30. S. Müller, A. Straub, S. Schröder, P.H. Bauer, M.J. Lohse, Interactions of phosducin with defined G protein βγ-subunits, J. Biol. Chem. 271 (1996) 11781-11786.
31. E.J. Neer, Heterotrimeric G proteins: organizers of transmembrane signals, Cell 80 (1995) 249-257.
32. K.R. Olson, J.R. Mcintosh, J.B. Olmsted, Analysis of MAP4 function in living cells using green fluorescent protein (GFP) chimeras, J. Cell Biol. 130 (1995) 639-650.
33. D.C. Prasher, V.K. Eckenrode, W.W. Ward, F.G. Prendergast, M.J. Cormier, Primary structure of the Aequorea victoria gree-fluorescent protein, Gene 111 (1992) 229-233.
34. R.T. Premont, J. Inglese, R.J. Lefkowitz, Protein kinases that phosphorylate activated G protein-coupled receptors, FASEB J. 9 (1995) 175-182.
35. N. Sakai, K. Sakai, N. Ikegaki, Y. Shirai, Y. Ono, N. Saito, Direct visualization of the translocation of the γ-subspecies of protein kinase C in living cells using fusion proteins with green fluorescent protein, J. Cell Biol. 139 (1997) 1465-1476.
36. K. Schulz, S. Danner, P. Bauer, S. Schröder, M.J. Lohse, Expression of phosducin in a phosducin-negative cell line reveals functions of a Gβγ-binding protein, J. Biol. Chem. 271 (1996) 22546-22551.
37. R. Sterne-Marr, J.L. Benovic, Regulation of G protein coupled receptors by receptor kinases and arrestins, Vit. Horm. 51 (1995) 193-233.
38. R.H. Strasser, J.L. Benovic, M.C. Caron, R.J. Lefkowitz, β-Agonist-and prostaglandin E1-induced translocation of the β-adrenergic receptor kinase: Evidence that the kinase may act on multiple adenylate cyclase-coupled receptors, Proc. Natl. Acad. Sci. USA 83 (1986) 6362-6366.
39. N.I. Tarasova, R.H. Stauber, J.K. Choi, E.A. Hudson, G. Czerwinski, J.L. Miller, G.N. Pavlakis, C.J. Michedja, S.A. Wank, Visualization of G protein-coupled receptor trafficking with the aid of the green fluorescence protein, J. Biol. Chem. 272 (1997) 14817-14824.
40. A. Wehmeyer, R. Schulz, Phosducin expression in NG 108-15 hybrid cells enhances prostaglandin E1 stimulated adenylate cyclase activity, Life Sci. (Acc. Comm.) in press.
41. M. Westphal, A. Jungbluth, M. Heidecker, B. Mühlbauer, C. Heizer, J.M. Schwartz, G. Marriott, G. Gerisch, Microfilament dynamics during cell movement and chemotaxis monitored using a GFP-actin fusion protein, Curr. Biol. 7 (1997) 176-183.
42. Z. Xiao, N. Zhang, D.B. Murphy, P.N. Decreotes, Dynamic distribution of chemoattractant receptors in living cells during chemotaxis and persistent stimulation, J. Cell Biol. 139 (1997) 365-374.
43. J. Xu, D. Wu, V.Z. Slepak, M.I. Simon, The N terminus of phosducin is involved in binding of beta gamma subunits of G protein, Proc. Natl. Acad. Sci. USA 92 (1995) 2086-2090.
44. F. Yang, L.G. Moss, G.N. Phillips, The molecular structure of green fluorescent protein, Nature Biotech. 14 (1996) 1246-1251.