A structural role for glutamine 214 in human thymidylate synthase

Biochemistry

Volumn 37, Issue 20, 1998, Pages 7089-7095

  Steadman, David J ^a   Zhao, Pei Shan ^a,b   Spencer, H Trent ^a   Dunlap, R Bruce ^a   Berger, Sondra H ^a  

^a UNIVERSITY OF SOUTH CAROLINA   (United States)

^b ST ELIZABETH'S MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

THYMIDYLATE SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME STRUCTURE; HYDROGEN BOND; HYDROPHOBICITY; LIGAND BINDING; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION;

AMINO ACID SUBSTITUTION; BINDING SITES; CELL LINE; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; GLUTAMINE; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; THYMIDYLATE SYNTHASE;

BACTERIA (MICROORGANISMS); FELIS CATUS;

EID: 0032546546     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9725428     Document Type: Article

References (35)

1. Carreras, C. W., and Santi, D. V. (1995) Annu. Rev. Biochem. 64, 721-762.
2. Cisneros, R. J., Silks, L. A., and Dunlap, R. B. (1988) Drugs Future 9, 859-881.
3. Hardy, L. W., Finer-Moore, J. S., Montfort, W. R., Jones, M. O., Santi, D. V., and Stroud, R. M. (1987) Science 235, 448-455.
4. Matthews, D. A., Appelt, K., Oatley, S. J., and Xuong, Ng. H. (1990) J. Mol. Biol. 214, 923-936.
5. Finer-Moore, J. S., Montfort, W. R., and Stroud, R. M. (1990) Biochemistry 29, 6977-6986.
6. Lapat-Polasko, L., Maley, G. F., and Maley F. (1990) Biochemistry 29, 9561-9572.
7. Climie, S., Ruiz-Perez, L., Gonzalez-Pacanowska, D., Prapunwattana, P., Cho, S.-W., Stroud, R., and Santi, D. V. (1990) J. Biol. Chem. 265, 18776-18779.
8. Michaels, M. L., Kim, C. W., Matthews, D. A., and Miller, J. H. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 3957-3961.
9. Perry, K. M., Fauman, E. B., Finer-Moore, J. S., Montfort, W. R., Maley, G. F., Maley, F., and Stroud, R. M. (1990) Proteins 8, 315-333.
10. Matthews, D. A., Appell, K., and Oatley, S. J. (1989) J. Mol. Biol. 205, 449-454.
11. Montfort, W. R., Perry, K. M., Fauman, E. B., Finer-Moore, J. S., Maley, G. F., Hardy, L., Maley, F., and Stroud, R. M. (1990) Biochemistry 29, 6964-6977.
12. Matthews, D. A., Villafranca, J. E., Janson, C. A., Smith, W. W., Welsh, K., and Freer, S. (1990) J. Mol. Biol. 214, 937-948.
13. Finer-Moore, J. S., Liu, L., Schafmeister, C. E., Birdsall, D. L., Mau, T., Santi, D. V., and Stroud, R. M. (1996) Biochemistry 35, 5125-5136.
14. Dunlap, R. B., Harding, N. G. L., and Huennekens, F. M. (1971) Biochemistry 10, 88-97.
15. Dev, I. K., Yates, B. B., Leong, J., and Dallas, W. S. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 1472-1476.
16. Dev, I. K., Dallas, W. S., Ferone, R., Hanlon, M., McKee, D. D., and Yates, B. B. (1994) J. Biol. Chem. 269, 1873-1882.
17. Harvey, R. J. (1970) J. Bacteriol. 101, 574-583.
18. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1989) in Molecular Cloning: A Laboratory Manual, 2nd ed., pp 1.74-
19. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Takeishi, K., Kaneda, S., Ayusawa, D., Shimizu, K., Gotoh, O., and Seno, T. (1985) Nucleic Acids Res. 13, 2035-2043.
21. Kulaga, H., and Sogn, J. A. (1985) J. Tissue Cult. Methods 9, 151-153.
22. Wahba, A. J., and Friedkin, M. (1961) J. Biol. Chem. 236, PC11-PC12.
23. Ellis, K. J., and Morrison, J. F. (1982) Methods Enzymol. 87, 405-426.
24. Lockshin, A., and Danenberg, P. V. (1981) Biochem. Pharmacol. 30, 247-257.
25. Spencer, H. T., Villafranca, J. E., and Appleman, J. R. (1997) Biochemistry 36, 4212-4222.
26. Reilly, R. T., Barbour, K. W., Dunlap, R. B., and Berger, F. G. (1995) Mol. Pharmacol. 48, 72-79.
27. Davisson, V. J., Sirawaraporn, W., and Santi, D. V. (1989) J. Biol. Chem. 264, 9145-9148.
28. Cornette, J. L., Cease, K. B., Margalit, H., Spouge, J. L., Berzofsky, J. A., and DeLisi, C. (1987) J. Mol. Biol. 195, 659-685.
29. Richardson, J. S., Getzoff, E. D., and Richardson, D. C. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 2574-2578.
30. Climie, S. C., Carreras, C. W., and Santi, D. V. (1992) Biochemistry 31, 6032-6038.
31. Schiffer, C. A., Clifton, I. J., Davisson, V. J., Santi, D. V., and Stroud, R. M. (1995) Biochemistry 34, 16279-16287.
32. Dev, I. K., Yates, B. B., Atashi, J., and Dallas, W. S. (1989) J. Biol. Chem. 264, 19132-19137.
33. Liu, L., and Santi, D. V. (1992) Biochemistry 31, 5100-5104.
34. Fersht, A. R. (1985) in Enzyme Structure and Mechanism, 2nd ed., W. H. Freeman and Company, New York.
35. Chothia, C. (1984) Annu. Rev. Biochem. 53, 537-572.