Fertilization defects in sperm from mice lacking fertilin β

Science

Volumn 281, Issue 5384, 1998, Pages 1857-1859

  Cho, Chunghee ^a   Bunch, Donna O'Dell ^b   Faure, Jean Emmanuel ^a,c   Goulding, Eugenia H ^b   Eddy, Edward M ^b   Primakoff, Paul ^a   Myles, Diana C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

^c UNIVERSITÉ DE LYON   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE PROTEIN; FERTILIN; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; FERTILIZATION; MALE; MALE INFERTILITY; MEMBRANE BINDING; MOUSE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; SPERM; SPERMATOGENESIS; SPERMATOZOON MOTILITY; ZONA PELLUCIDA;

ADAM PROTEINS; ANIMALS; CALCIUM; CELL ADHESION; CELL MEMBRANE; FALLOPIAN TUBES; FEMALE; MALE; MEMBRANE FUSION; MEMBRANE GLYCOPROTEINS; METALLOENDOPEPTIDASES; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; OVUM; SPERM CAPACITATION; SPERM-OVUM INTERACTIONS; SPERMATOGENESIS; SPERMATOZOA; ZONA PELLUCIDA;

ANIMALIA; MAMMALIA;

EID: 0032544623     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5384.1857     Document Type: Article

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30. Each male (2 to 4 months old) was placed with one to two females (C57BL6/J or C57BL6/N) for 5 days to 2 months. One group of mated females was killed to count the number of fetuses at 10 to 15 days after the first day of mating. From the other group of females, the number of pups born was counted.
31. 1 progeny carrying the targeted fertilin β gene.
32. Female mice (C57BL6/J or C57BL6/N) were primed with hormones and mated with males as described above. At 1.5 to 7 hours after a plug was found, the oviduct was dissected out of the females and flushed or diced in Whittingham's medium to release sperm and cumulus masses with eggs. The presence of sperm was determined by counting sperm that were fused with eggs, bound to the egg surface, found with cumulus cells, and found free in the oviduct.
33. Animal care was in accordance with U.S. Public Health Service guidelines for use of animals, and all procedures were approved in advance by the Institutional Animal Care and Use Committee of the University of California, Davis, and the National Institute of Environmental Health Science. We thank B. Miller, R. Yuan, A. Yudin, K. Robertson, W. Willis, B. Koller, V. Valancius-Mangel, M. Capecchi, M. McBurney, B. Shur, and Syntex (Palo Alto, CA) for providing reagents and help with particular experiments. This work was supported by grants to D.G.M. (HD16580) and P.P. (U54HD29125) from NIH.