Cathepsin L: Critical role in Ii degradation and CD4 T cell selection in the thymus

Science

Volumn 280, Issue 5362, 1998, Pages 450-453

  Nakagawa, Terry ^a   Roth, Wera ^b   Wong, Phillip ^a   Nelson, Andrew ^a   Farr, Andrew ^a   Deussing, Jan ^b   Villadangos, Jose A ^c   Ploegh, Hidde ^c   Peters, Christoph ^b   Rudensky, Alexander Y ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF FREIBURG   (Germany)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATHEPSIN L; CD4 ANTIGEN; CYSTEINE PROTEINASE; IA ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2;

ANIMAL CELL; ANTIGEN PRESENTATION; ANTIGEN PRESENTING CELL; ARTICLE; CONTROLLED STUDY; HELPER CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; THYMOCYTE;

ANIMALS; ANTIGEN PRESENTATION; ANTIGEN-PRESENTING CELLS; ANTIGENS, DIFFERENTIATION, B-LYMPHOCYTE; BONE MARROW CELLS; CATHEPSINS; CD4 LYMPHOCYTE COUNT; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CYSTEINE ENDOPEPTIDASES; ENDOPEPTIDASES; EPITHELIAL CELLS; HISTOCOMPATIBILITY ANTIGENS CLASS II; MICE; MICE, INBRED C57BL; MUTATION; SPLEEN; T-LYMPHOCYTE SUBSETS; THYMUS GLAND;

ANIMALIA;

EID: 0032540474     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5362.450     Document Type: Article

References (39)

1. P. R. Wolf and H. L. Ploegh, Annu. Rev. Cell Dev. Biol. 11, 267 (1995).
2. V. H. Engelhard, Annu. Rev. Immunol. 12, 181 (1994).
3. P. J. Fink and M. J. Bevan, Adv. Immunol. 59, 99 (1995).
4. J. S. Blum and P. Cresswell, Proc. Natl. Acad. Sci. U.S.A. 85, 3975 (1988); J. J. Neefjes and H. L. Ploegh, EMBO J. 11, 411 (1992); M. A. Maric, M. D. Taylor, J. S. Blum. Proc. Natl. Acad. Sci. U.S.A. 91, 2171 (1994); R. R. Avva and P. Cresswell, Immunity 1, 763 (1994); J. M. van Noort and M. J. Jacobs, Eur. J. Immunol. 24, 2175 (1994); G. M. Rodriguez and S. Diment, ibid. 25, 1823 (1995); T. Bevec, V. Stoka, G. Pungercic, I. Dolenc, V. Turk, J. Exp. Med. 183, 1331 (1996); B. Fineschi, K. Sakaguchi, E. Appella, J. Miller, J. Immunol. 157, 3211 (1996).
5. J. S. Blum and P. Cresswell, Proc. Natl. Acad. Sci. U.S.A. 85, 3975 (1988); J. J. Neefjes and H. L. Ploegh, EMBO J. 11, 411 (1992); M. A. Maric, M. D. Taylor, J. S. Blum. Proc. Natl. Acad. Sci. U.S.A. 91, 2171 (1994); R. R. Avva and P. Cresswell, Immunity 1, 763 (1994); J. M. van Noort and M. J. Jacobs, Eur. J. Immunol. 24, 2175 (1994); G. M. Rodriguez and S. Diment, ibid. 25, 1823 (1995); T. Bevec, V. Stoka, G. Pungercic, I. Dolenc, V. Turk, J. Exp. Med. 183, 1331 (1996); B. Fineschi, K. Sakaguchi, E. Appella, J. Miller, J. Immunol. 157, 3211 (1996).
6. J. S. Blum and P. Cresswell, Proc. Natl. Acad. Sci. U.S.A. 85, 3975 (1988); J. J. Neefjes and H. L. Ploegh, EMBO J. 11, 411 (1992); M. A. Maric, M. D. Taylor, J. S. Blum. Proc. Natl. Acad. Sci. U.S.A. 91, 2171 (1994); R. R. Avva and P. Cresswell, Immunity 1, 763 (1994); J. M. van Noort and M. J. Jacobs, Eur. J. Immunol. 24, 2175 (1994); G. M. Rodriguez and S. Diment, ibid. 25, 1823 (1995); T. Bevec, V. Stoka, G. Pungercic, I. Dolenc, V. Turk, J. Exp. Med. 183, 1331 (1996); B. Fineschi, K. Sakaguchi, E. Appella, J. Miller, J. Immunol. 157, 3211 (1996).
7. J. S. Blum and P. Cresswell, Proc. Natl. Acad. Sci. U.S.A. 85, 3975 (1988); J. J. Neefjes and H. L. Ploegh, EMBO J. 11, 411 (1992); M. A. Maric, M. D. Taylor, J. S. Blum. Proc. Natl. Acad. Sci. U.S.A. 91, 2171 (1994); R. R. Avva and P. Cresswell, Immunity 1, 763 (1994); J. M. van Noort and M. J. Jacobs, Eur. J. Immunol. 24, 2175 (1994); G. M. Rodriguez and S. Diment, ibid. 25, 1823 (1995); T. Bevec, V. Stoka, G. Pungercic, I. Dolenc, V. Turk, J. Exp. Med. 183, 1331 (1996); B. Fineschi, K. Sakaguchi, E. Appella, J. Miller, J. Immunol. 157, 3211 (1996).
8. J. S. Blum and P. Cresswell, Proc. Natl. Acad. Sci. U.S.A. 85, 3975 (1988); J. J. Neefjes and H. L. Ploegh, EMBO J. 11, 411 (1992); M. A. Maric, M. D. Taylor, J. S. Blum. Proc. Natl. Acad. Sci. U.S.A. 91, 2171 (1994); R. R. Avva and P. Cresswell, Immunity 1, 763 (1994); J. M. van Noort and M. J. Jacobs, Eur. J. Immunol. 24, 2175 (1994); G. M. Rodriguez and S. Diment, ibid. 25, 1823 (1995); T. Bevec, V. Stoka, G. Pungercic, I. Dolenc, V. Turk, J. Exp. Med. 183, 1331 (1996); B. Fineschi, K. Sakaguchi, E. Appella, J. Miller, J. Immunol. 157, 3211 (1996).
9. J. S. Blum and P. Cresswell, Proc. Natl. Acad. Sci. U.S.A. 85, 3975 (1988); J. J. Neefjes and H. L. Ploegh, EMBO J. 11, 411 (1992); M. A. Maric, M. D. Taylor, J. S. Blum. Proc. Natl. Acad. Sci. U.S.A. 91, 2171 (1994); R. R. Avva and P. Cresswell, Immunity 1, 763 (1994); J. M. van Noort and M. J. Jacobs, Eur. J. Immunol. 24, 2175 (1994); G. M. Rodriguez and S. Diment, ibid. 25, 1823 (1995); T. Bevec, V. Stoka, G. Pungercic, I. Dolenc, V. Turk, J. Exp. Med. 183, 1331 (1996); B. Fineschi, K. Sakaguchi, E. Appella, J. Miller, J. Immunol. 157, 3211 (1996).
10. J. S. Blum and P. Cresswell, Proc. Natl. Acad. Sci. U.S.A. 85, 3975 (1988); J. J. Neefjes and H. L. Ploegh, EMBO J. 11, 411 (1992); M. A. Maric, M. D. Taylor, J. S. Blum. Proc. Natl. Acad. Sci. U.S.A. 91, 2171 (1994); R. R. Avva and P. Cresswell, Immunity 1, 763 (1994); J. M. van Noort and M. J. Jacobs, Eur. J. Immunol. 24, 2175 (1994); G. M. Rodriguez and S. Diment, ibid. 25, 1823 (1995); T. Bevec, V. Stoka, G. Pungercic, I. Dolenc, V. Turk, J. Exp. Med. 183, 1331 (1996); B. Fineschi, K. Sakaguchi, E. Appella, J. Miller, J. Immunol. 157, 3211 (1996).
11. J. S. Blum and P. Cresswell, Proc. Natl. Acad. Sci. U.S.A. 85, 3975 (1988); J. J. Neefjes and H. L. Ploegh, EMBO J. 11, 411 (1992); M. A. Maric, M. D. Taylor, J. S. Blum. Proc. Natl. Acad. Sci. U.S.A. 91, 2171 (1994); R. R. Avva and P. Cresswell, Immunity 1, 763 (1994); J. M. van Noort and M. J. Jacobs, Eur. J. Immunol. 24, 2175 (1994); G. M. Rodriguez and S. Diment, ibid. 25, 1823 (1995); T. Bevec, V. Stoka, G. Pungercic, I. Dolenc, V. Turk, J. Exp. Med. 183, 1331 (1996); B. Fineschi, K. Sakaguchi, E. Appella, J. Miller, J. Immunol. 157, 3211 (1996).
12. P. Saftig et al., EMBO J. 14, 3599 (1995).
13. W. Roth et al., in preparation.
14. S. Eastman et al., Eur. J. Immunol. 26, 385 (1996).
15. W.-P. Fung-Leung et al., Science 271, 1278 (1996); T. Miyazaki et al., Cell 84, 531 (1996); W. D. Martin et al., ibid., p. 543.
16. W.-P. Fung-Leung et al., Science 271, 1278 (1996); T. Miyazaki et al., Cell 84, 531 (1996); W. D. Martin et al., ibid., p. 543.
17. W.-P. Fung-Leung et al., Science 271, 1278 (1996); T. Miyazaki et al., Cell 84, 531 (1996); W. D. Martin et al., ibid., p. 543.
18. T. Y. Nakagawa, W. Roth, C. Peters, A. Y. Rudensky, unpublished data.
19. R. J. Riese et al., Immunity 4, 357 (1996); J. A. Villadangos, R. J. Riese, C. Peters, H. A. Chapman, H. L. Ploegh, J. Exp. Med. 186, 549 (1997).
20. R. J. Riese et al., Immunity 4, 357 (1996); J. A. Villadangos, R. J. Riese, C. Peters, H. A. Chapman, H. L. Ploegh, J. Exp. Med. 186, 549 (1997).
21. S. Morkowski et al., J. Exp. Med. 182, 1403 (1995).
22. N. Koch, S. Koch, G. J. Hammerling, Nature 299, 644 (1982).
23. M. Liljedahl et al., EMBO J. 15, 4817 (1996); L. K. Denzin, D. B. Sant'Angelo, C. Hammond, M. J. Surman, P. Cresswell, Science 278, 106 (1997); T. Nakagawa and A. Y. Rudensky, data not shown.
24. M. Liljedahl et al., EMBO J. 15, 4817 (1996); L. K. Denzin, D. B. Sant'Angelo, C. Hammond, M. J. Surman, P. Cresswell, Science 278, 106 (1997); T. Nakagawa and A. Y. Rudensky, data not shown.
25. M. Liljedahl et al., EMBO J. 15, 4817 (1996); L. K. Denzin, D. B. Sant'Angelo, C. Hammond, M. J. Surman, P. Cresswell, Science 278, 106 (1997); T. Nakagawa and A. Y. Rudensky, data not shown.
26. C. D. Surh, L. Dong-Sup, W.-P. Fung-Leung, L. Karlsson, J. Sprent, Immunity 7, 209 (1997); S. Tourne et al., ibid., p. 187.
27. C. D. Surh, L. Dong-Sup, W.-P. Fung-Leung, L. Karlsson, J. Sprent, Immunity 7, 209 (1997); S. Tourne et al., ibid., p. 187.
28. P. Marrack and J. Kappler, Science 238, 1073 (1987).
29. A. Farr, P. C. DeRoos, S. Eastman, A. Y. Rudensky, Eur. J. Immunol. 26, 3185 (1996).
30. B. Adkins, G. F. Tidmarsh, I. L. Weissman, Immunogenetics 27, 180 (1988).
31. -/- littermates showed normal phenotype and were used as controls.
32. 600).
33. Thymocytes and SCs were stained with anti-CD4-phycoerythrin (PE), anti-CD8α-fluorescein isothiocyanate (FITC), and anti-TCRβ-biotin mAbs (Pharmingen) followed by streptavidin-Tricolor and analyzed by a FACScan flow cytometer (Becton-Dickinson).
34. T. Nakagawa, P. Wong, A. Y. Rudensky, data not shown.
35. 6 T cell-depleted BM cells into lethally irradiated (950 rads) recipients. Thymocytes and SCs were analyzed for CD4, CD8, and TCRβ expression 6 to 8 weeks after reconstitution. Reconstitution of recipients by donor BM was greater than 90% as assessed by staining with anti-Ly9.1 oranti-Ly5.1 mAb (Pharmingen).
36. 2 (Fig. 4). Cells were washed, lysed, and analyzed by 12% SDS-polyacrylamide gel electrophoresis (PAGE).
37. R. W. Mason, D. Wilcox, P. Wikstrom, E. N. Shaw, Biochem. J. 257, 125 (1989).
38. b, showed minimal background staining.
39. We thank D. Wilson, S. Eastman, E. Rudensky, and P. deRoos for technical assistance; H. Caldwell for C. trachomatis and 116.3 T hybrids; S. Kahn for recombinant SA85 T. cruzi protein and 71.5 T hybrids; N. Shastri for BO4 T hybrids; M. Bevan and P. Fink for reading the manuscript; and members of our laboratories for helpful discussions. Supported by the Howard Hughes Medical Institute and by NIH (A.Y.R.).