-
1
-
-
0029072047
-
-
V. S. Sloan et al., Nature 375, 802 (1995); L. K. Denzin and P Cresswell, Cell 82, 155 (1995); M. A. Sherman, D. A. Weber, P. A. Jensen, Immunity 3, 197 (1995).
-
(1995)
Nature
, vol.375
, pp. 802
-
-
Sloan, V.S.1
-
2
-
-
0028981178
-
-
V. S. Sloan et al., Nature 375, 802 (1995); L. K. Denzin and P Cresswell, Cell 82, 155 (1995); M. A. Sherman, D. A. Weber, P. A. Jensen, Immunity 3, 197 (1995).
-
(1995)
Cell
, vol.82
, pp. 155
-
-
Denzin, L.K.1
Cresswell, P.2
-
3
-
-
0029084023
-
-
V. S. Sloan et al., Nature 375, 802 (1995); L. K. Denzin and P Cresswell, Cell 82, 155 (1995); M. A. Sherman, D. A. Weber, P. A. Jensen, Immunity 3, 197 (1995).
-
(1995)
Immunity
, vol.3
, pp. 197
-
-
Sherman, M.A.1
Weber, D.A.2
Jensen, P.A.3
-
4
-
-
0028899617
-
-
C C. Stebbins, G. Loss Jr., C. G. Elias, A. Chervonsky, A. J. Sant, J. Exp. Med. 181, 223 (1995); G Brooks, P L Campbell, P. Reynolds, A M. Gautam, J. McCluskey, J. Immunol. 153, 5382 (1994).
-
(1995)
J. Exp. Med.
, vol.181
, pp. 223
-
-
Stebbins, C.C.1
Loss Jr., G.2
Elias, C.G.3
Chervonsky, A.4
Sant, A.J.5
-
5
-
-
0028135019
-
-
C C. Stebbins, G. Loss Jr., C. G. Elias, A. Chervonsky, A. J. Sant, J. Exp. Med. 181, 223 (1995); G Brooks, P L Campbell, P. Reynolds, A M. Gautam, J. McCluskey, J. Immunol. 153, 5382 (1994).
-
(1994)
J. Immunol.
, vol.153
, pp. 5382
-
-
Brooks, G.1
Campbell, P.L.2
Reynolds, P.3
Gautam, A.M.4
McCluskey, J.5
-
6
-
-
0025056497
-
-
E. Mellins et al., Nature 343, 71 (1990); J. M. Riberdy, J. R Newcomb, M. J. Surman, J. A. Barbosa, P. Cresswell, ibid. 360, 474 (1992); P. Morris et al., ibid. 368, 551 (1994); P. Fling, B. Arp, D. Pious, ibid., p. 554
-
(1990)
Nature
, vol.343
, pp. 71
-
-
Mellins, E.1
-
7
-
-
0026440236
-
-
E. Mellins et al., Nature 343, 71 (1990); J. M. Riberdy, J. R Newcomb, M. J. Surman, J. A. Barbosa, P. Cresswell, ibid. 360, 474 (1992); P. Morris et al., ibid. 368, 551 (1994); P. Fling, B. Arp, D. Pious, ibid., p. 554
-
(1992)
Nature
, vol.360
, pp. 474
-
-
Riberdy, J.M.1
Newcomb, J.R.2
Surman, M.J.3
Barbosa, J.A.4
Cresswell, P.5
-
8
-
-
0028350714
-
-
E. Mellins et al., Nature 343, 71 (1990); J. M. Riberdy, J. R Newcomb, M. J. Surman, J. A. Barbosa, P. Cresswell, ibid. 360, 474 (1992); P. Morris et al., ibid. 368, 551 (1994); P. Fling, B. Arp, D. Pious, ibid., p. 554
-
(1994)
Nature
, vol.368
, pp. 551
-
-
Morris, P.1
-
9
-
-
0025056497
-
-
E. Mellins et al., Nature 343, 71 (1990); J. M. Riberdy, J. R Newcomb, M. J. Surman, J. A. Barbosa, P. Cresswell, ibid. 360, 474 (1992); P. Morris et al., ibid. 368, 551 (1994); P. Fling, B. Arp, D. Pious, ibid., p. 554
-
Nature
, pp. 554
-
-
Fling, P.1
Arp, B.2
Pious, D.3
-
10
-
-
13344284773
-
-
note
-
b and thus express only H2-A, not H2-E
-
-
-
-
11
-
-
13344262157
-
-
note
-
β.
-
-
-
-
12
-
-
0028618339
-
-
L. Karlsson, A. Péléraux, R. Lindstedt, M. Liljedahl, P. A. Peterson, Science 266, 1569 (1994).
-
(1994)
Science
, vol.266
, pp. 1569
-
-
Karlsson, L.1
Péléraux, A.2
Lindstedt, R.3
Liljedahl, M.4
Peterson, P.A.5
-
13
-
-
13344279774
-
-
note
-
4Cl and PBS Antibody incubations were made in PBS with 0.6% fish skin gelatin and 0.2% saponin for permeabilization. Texas-Red-labeled rabbit antibody to immunoglobulin G (IgG) (Molecular Probes) and fluorescein isothiocyanate (FITC)-labeled rat antibody to IgG (Cappel) were used as secondary reagents Fluorescent cells were imaged with the use of a Bio-Rad confocal microscope.
-
-
-
-
14
-
-
13344269811
-
-
note
-
b was precipitated with M5/ 114 (27). Immunoprecipitates were harvested with protein G-sepharose, washed, and resuspended in iscelectric focusing (IEF) sample buffer (Fig. 1E) or SDS-PAGE sample buffer containing 2% SDS without (Fig. 2C) or with 10 mM dithiothreitol (Fig. 2D). Samples were left at room temperature for 20 min (Fig. 2C) or boiled for 5 min (Fig. 2D). Samples were separated on 7.5 to 12.5% polyacrylamide gels directly or after IEF (pH 5 to 7). The gels were then fixed, dried, and autoradiographed. Autoradiographs were scanned with an Agfa Arcusll scanner. Composites were printed on a Kodak XLS 8600 printer.
-
-
-
-
15
-
-
13344282882
-
-
note
-
b, which suggests that the binding affinity for this complex is low.
-
-
-
-
17
-
-
13344279562
-
-
note
-
b, with the use of biotinylated M5/114 or BP107; and for CLIP, with the use of biotinylated 30-2 mAbs. Bound antibodies were detected with alkaline phosphatase-conjugated streptavidin (Jackson ImmunoResearch) followed by colorimetric substrate as described (25).
-
-
-
-
18
-
-
0026571278
-
-
L J. Stern and D. C. Wiley, Cell 68, 465 (1992); S. Sadegh-Nasseri and R. N. Germain, Nature 353, 167 (1991); C. A. Nelson, S. J. Petzold, E. R. Unanue, ibid. 371, 250 (1994).
-
(1992)
Cell
, vol.68
, pp. 465
-
-
Stern, L.J.1
Wiley, D.C.2
-
19
-
-
0025870669
-
-
L J. Stern and D. C. Wiley, Cell 68, 465 (1992); S. Sadegh-Nasseri and R. N. Germain, Nature 353, 167 (1991); C. A. Nelson, S. J. Petzold, E. R. Unanue, ibid. 371, 250 (1994).
-
(1991)
Nature
, vol.353
, pp. 167
-
-
Sadegh-Nasseri, S.1
Germain, R.N.2
-
20
-
-
0028064707
-
-
L J. Stern and D. C. Wiley, Cell 68, 465 (1992); S. Sadegh-Nasseri and R. N. Germain, Nature 353, 167 (1991); C. A. Nelson, S. J. Petzold, E. R. Unanue, ibid. 371, 250 (1994).
-
(1994)
Nature
, vol.371
, pp. 250
-
-
Nelson, C.A.1
Petzold, S.J.2
Unanue, E.R.3
-
21
-
-
0027536939
-
-
S. Viville et al., Cell 72, 635 (1993); K. Bikoff et al., J. Exp. Med. 177. 1699 (1993); K. Dornmair, B. Rothenhäusler, H. M. McConnell, Cold Spring Harbor Symp. Quant. Biol. 54, 409 (1989).
-
(1993)
Cell
, vol.72
, pp. 635
-
-
Viville, S.1
-
22
-
-
0027309316
-
-
S. Viville et al., Cell 72, 635 (1993); K. Bikoff et al., J. Exp. Med. 177. 1699 (1993); K. Dornmair, B. Rothenhäusler, H. M. McConnell, Cold Spring Harbor Symp. Quant. Biol. 54, 409 (1989).
-
(1993)
J. Exp. Med.
, vol.177
, pp. 1699
-
-
Bikoff, K.1
-
23
-
-
0024953878
-
-
S. Viville et al., Cell 72, 635 (1993); K. Bikoff et al., J. Exp. Med. 177. 1699 (1993); K. Dornmair, B. Rothenhäusler, H. M. McConnell, Cold Spring Harbor Symp. Quant. Biol. 54, 409 (1989).
-
(1989)
Cold Spring Harbor Symp. Quant. Biol.
, vol.54
, pp. 409
-
-
Dornmair, K.1
Rothenhäusler, B.2
McConnell, H.M.3
-
25
-
-
0028921633
-
-
A. Sette, S. Southwood, J. Miller, E. Appella, J. Exp. Med. 181, 677 (1995).
-
(1995)
J. Exp. Med.
, vol.181
, pp. 677
-
-
Sette, A.1
Southwood, S.2
Miller, J.3
Appella, E.4
-
26
-
-
13344285504
-
-
note
-
Immuprecipitation with mAb In-1, which is reactive with the invariant chain (but not with CLIP), did not reveal any differences between mutant and wild-type cells in either boiled or nonboiled samples.
-
-
-
-
27
-
-
13344276015
-
-
unpublished data
-
C. D. Surh, unpublished data.
-
-
-
Surh, C.D.1
-
28
-
-
0028823585
-
-
P. Ghosh, M. Amaya, E. Mellins, D. C. Wiley, Nature 378, 457 (1995).
-
(1995)
Nature
, vol.378
, pp. 457
-
-
Ghosh, P.1
Amaya, M.2
Mellins, E.3
Wiley, D.C.4
-
37
-
-
13344275313
-
-
note
-
hi, and few expressed markers associated with activation - for example, CD69 or interleukin-2 receptor.
-
-
-
-
38
-
-
13344276740
-
-
note
-
3H]thymidine for approximately 18 hours before harvesting and counting.
-
-
-
-
39
-
-
13344284772
-
-
note
-
We thank D. Uranowski and B. Marchand for secretarial assistance, L. Zhou, J. Tanakos, K. Ngo, M. Squier, and C. Fitzsimmons for technical assistance, G. Klier for confocal microscopy, A. Rudensky for 30-2 antibody, and J. Sprent for discussions and suggestions. C.D.S. is a Special Fellow of the Leukemia Society of America.
-
-
-
|