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Volumn 271, Issue 5253, 1996, Pages 1278-1281

Antigen presentation and T cell development in H2-M-deficient mice

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2;

EID: 0029945575     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5253.1278     Document Type: Article
Times cited : (247)

References (39)
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    • 4Cl and PBS Antibody incubations were made in PBS with 0.6% fish skin gelatin and 0.2% saponin for permeabilization. Texas-Red-labeled rabbit antibody to immunoglobulin G (IgG) (Molecular Probes) and fluorescein isothiocyanate (FITC)-labeled rat antibody to IgG (Cappel) were used as secondary reagents Fluorescent cells were imaged with the use of a Bio-Rad confocal microscope.
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    • b was precipitated with M5/ 114 (27). Immunoprecipitates were harvested with protein G-sepharose, washed, and resuspended in iscelectric focusing (IEF) sample buffer (Fig. 1E) or SDS-PAGE sample buffer containing 2% SDS without (Fig. 2C) or with 10 mM dithiothreitol (Fig. 2D). Samples were left at room temperature for 20 min (Fig. 2C) or boiled for 5 min (Fig. 2D). Samples were separated on 7.5 to 12.5% polyacrylamide gels directly or after IEF (pH 5 to 7). The gels were then fixed, dried, and autoradiographed. Autoradiographs were scanned with an Agfa Arcusll scanner. Composites were printed on a Kodak XLS 8600 printer.
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    • Immuprecipitation with mAb In-1, which is reactive with the invariant chain (but not with CLIP), did not reveal any differences between mutant and wild-type cells in either boiled or nonboiled samples.
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    • hi, and few expressed markers associated with activation - for example, CD69 or interleukin-2 receptor.
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    • 3H]thymidine for approximately 18 hours before harvesting and counting.
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    • note
    • We thank D. Uranowski and B. Marchand for secretarial assistance, L. Zhou, J. Tanakos, K. Ngo, M. Squier, and C. Fitzsimmons for technical assistance, G. Klier for confocal microscopy, A. Rudensky for 30-2 antibody, and J. Sprent for discussions and suggestions. C.D.S. is a Special Fellow of the Leukemia Society of America.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.