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Volumn 8, Issue 6, 1998, Pages 601-606

Selective photocleavage of single-stranded nucleic acids by cyclobisintercaland molecules

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; OLIGONUCLEOTIDE; REAGENT; SINGLE STRANDED DNA; TRANSFER RNA;

EID: 0032539789     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(98)00085-7     Document Type: Article
Times cited : (11)

References (25)
  • 1
    • 0003121215 scopus 로고
    • (Eds.: F. Eckstein and D. M. J. Lilley), Springer, Berlin
    • 1. Dervan, P. B., in Nucleic Acids and Molecular Biology, (Eds.: F. Eckstein and D. M. J. Lilley), Springer, Berlin, 1988, 2, 49.
    • (1988) Nucleic Acids and Molecular Biology , vol.2 , pp. 49
    • Dervan, P.B.1
  • 7
    • 9844238186 scopus 로고
    • 5. Sigman, D. S.; Bruice, T. W.; Mazumder, A.; Sutton, C. L. Acc. Chem. Res. 1993, 26, 98; Sigman, D. S.; Mazumder, A.; Perrin, D. M. Chem. Rev. 1993, 93, 2295.
    • (1993) Chem. Rev. , vol.93 , pp. 2295
    • Sigman, D.S.1    Mazumder, A.2    Perrin, D.M.3
  • 11
    • 0010618878 scopus 로고
    • unpublished work; Blacker, A. J., Ph. D. Thesis, University Louis Pasteur, Strasbourg
    • 9. Blacker, A. J.; Lehn, J.-M., unpublished work; Blacker, A. J., Ph. D. Thesis, University Louis Pasteur, Strasbourg, 1988.
    • (1988)
    • Blacker, A.J.1    Lehn, J.-M.2
  • 19
    • 0010635546 scopus 로고    scopus 로고
    • note
    • 17 Irradiation experiments. a) With compound 2. Equimolar mixtures (0.06 mM in nucleotide phosphate units) of pBR322 and of M13mp19 in NaOAc buffer at pH = 4.4 were irradiated for 30 mn with visible light (>395 nm filter) in presence of different amounts of 2. Samples were loaded on a 1% agarose gel in a pH 8.0 Tris/EDTA buffer, submitted to electrophoresis and then stained with ethidium bromide. The DNA bands were revealed by UV light and a polaroid photograph taken; the amounts of DNA forms were quantified by reflectance densitometry. b) With compound 3. The DNA (pUC18 or M13mp18 or 1/1 mixtures of the two) solutions (0.3 mM in nucleotide phosphate units) were irradiated for 20 mn with 360 nm light (provided by the monochromator of a Spex Fluoromax fluorimeter) in Tris HC1 buffer (0.1 M, pH 7.0, total volume 10 μl) in the presence of concentrations of 3 from 0 to 15 μM ds pUC18 was analyzed by electrophoresis on a 1% agarose gel in 1M Tris-acetate buffer, whereas a 1.4% agarose gel in 0.5M Tris-borate buffer was preferred for ss M13mp8 and for experiments on the mixtures of NAs, these conditions providing a much better separation of the circular and linear forms. In both cases the extent of cleavage has been quantified by the disappearance of the starting material (forms 1 or C, Fig. 3) with respect to the control.
  • 24
    • 0023938317 scopus 로고
    • 22. For selective strand scission at bulges by intercalating drugs, see for instance also: Williams, L.D.; Goldberg, I. H. Biochemistry 1988, 27, 3004.
    • (1988) Biochemistry , vol.27 , pp. 3004
    • Williams, L.D.1    Goldberg, I.H.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.