Tic20 and Tic22 are new components of the protein import apparatus at the chloroplast inner envelope membrane

Journal of Cell Biology

Volumn 143, Issue 4, 1998, Pages 991-1002

  Kouranov, Andrei ^a   Chen, Xuejun ^a   Fuks, Bruno ^a   Schnell, Danny J ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

Chloroplast; Membrane translocation; Protein import; Protein targeting

Indexed keywords

ENVELOPE PROTEIN; MEMBRANE PROTEIN; PROTEIN TIC20; PROTEIN TIC22; UNCLASSIFIED DRUG;

ARTICLE; CHLOROPLAST; COVALENT BOND; ENVELOPE GENE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; QUANTITATIVE DIAGNOSIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CHLOROPLASTS; CHROMATOGRAPHY, AFFINITY; INTRACELLULAR MEMBRANES; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; PEAS; PLANT PROTEINS;

EID: 0032539010     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.143.4.991     Document Type: Article

References (35)

1. Akita, M., E. Nielsen, and K. Keegstra. 1997. Identification of protein transport complexes in the chloroplastic envelope membranes via chemical cross-linking. J. Cell Biol. 136:983-994.
2. Anderson, D.J., and G. Blobel. 1983. Immunoprecipitation of proteins from cell-free translations. Methods Enzymol. 96:111-120.
3. Caliebe, A., R. Grimm, G. Kaiser, J. Lübeck, J. Soll, and L. Heins. 1997. The chloroplastic protein import machinery contains a Rieske-type iron-sulfur cluster and a mononuclear iron-binding protein. EMBO (Eur. Mol. Biol. Organ.) J. 16:7342-7350.
4. Cline, K., and R. Henry. 1996. Import and routing of nucleus-encoded chloroplast proteins. Annu. Rev. Cell Dev. Biol. 12:1-26.
5. Cline, K., M. Werner-Washburne, J. Andrews, and K. Keegstra. 1984. Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts. Plant Physiol. 74:675-678.
6. Cremers, F.F.M., W.F. Voorhout, T.P. van der Krift, J.J.M. Leunissen-Bijvelt, and A.J. Verkleij. 1988. Visualization of contact sites between outer and inner envelope membranes in isolated chloroplasts. Biochim. Biophys. Acta. 933:334-340.
7. Fernandez, J., M. DeMott, D. Atherton, and S.M. Mische. 1992. Internal protein sequence analysis: enzymatic digestion for less than 10 micrograms of protein bound to polyvinylidene difluoride or nitrocellulose membranes. Anal. Biochem. 210:255-264.
8. Fliege, R., U.-I. Flügge, K. Werdan, and W.H. Heldt. 1978. Specific transport of inorganic phosphate, 3-phosphoglycerate and triosephosphates across the inner membrane of the envelope in spinach chloroplasts. Biochim. Biophys. Acta. 502:232-247.
9. Fuks, B., and D.J. Schnell. 1997. Mechanism of protein transport across the chloroplast envelope. Plant Physiol. 114:405-410.
10. Gavel, Y., and G. von Heijne. 1990. A conserved cleavage-site motif in chloroplast transit peptides. FEBS (Fed. Eur. Biochem. Soc.) Lett. 261:455-458.
11. Hinnah, S.C., K. Hill, R. Wagner, T. Schlicher, and J. Soll. 1997. Reconstitution of a chloroplast protein import channel. EMBO (Eur. Mol. Biol. Organ.) J. 16:7351-7360.
12. Hirsch, S., E. Muckel, F. Heemeyer, G. von Heijne, and J. Soll. 1994. A receptor component of the chloroplast protein translocation machinery. Science. 266:1989-1992.
13. Horst, M., S. Hilfiker-Rothenfluh, W. Oppliger, and G. Schatz. 1995. Dynamic interaction of the protein translocation systems in the inner and outer membranes of yeast mitochondria. EMBO (Eur. Mol. Biol. Organ.) J. 14:2293-2297.
14. Jackson, D.T., J.E. Froehlich, and K. Keegstra. 1998. The hydrophilic domain of Tic110, an inner envelope membrane component of the chloroplastic protein translocation apparatus, faces the stromal compartment. J. Biol. Chem. 273:16583-16588.
15. Keegstra, K., and A.E. Yousif. 1986. Isolation and characterization of chloroplast envelope membranes. Methods Enzymol. 118:316-325.
16. Kessler, F., and G. Blobel. 1996. Interaction of the protein import and folding machineries in the chloroplast. Proc. Natl. Acad. Sci. USA. 93:7684-7689.
17. Kessler, F., G. Blobel, H.A. Patel, and D.J. Schnell. 1994. Identification of two GTP-binding proteins in the chloroplast protein import machinery. Science. 266:1035-1039.
18. Knight, J.S., and J.C. Gray. 1995. The N-terminal hydrophobic region of the mature phosphate translocator is sufficient for targeting to the chloroplast inner envelope membrane. Plant Cell. 7:1421-1432.
19. Kouranov, A., and D.J. Schnell. 1997. Analysis of the interactions of preproteins with the import machinery over the course of protein import into chloroplasts. J. Cell Biol. 139:1677-1685.
20. Lübeck, J., J. Soll, M. Akita, E. Nielsen, and K. Keegstra. 1996. Topology of IEP110, a component of the chloroplastic protein import machinery present in the inner envelope membrane. EMBO (Eur. Mol. Biol. Organ.) J. 15: 4230-4238.
21. Lübeck, J., L. Heins, and J. Soil. 1997. Protein import into chloroplasts. Physiol. Plant. 100:53-64.
22. Ma, Y., A. Kouranov, S. LaSala, and D.J. Schnell. 1996. Two components of the chloroplast protein import apparatus, IAP86 and IAP75, interact with the transit sequence during the recognition and translocation of precursor proteins at the outer envelope. J. Cell Biol. 134:1-13.
23. Nielsen, E., M. Akita, J. Davila-Aponte, and K. Keegstra. 1997. Stable association of chloroplastic precursors with protein-translocation complexes that contain proteins from both envelope membranes and a stromal Hsp100 molecular chaperone. EMBO (Eur. Mol. Biol. Organ.) J. 16:935-946.
24. Olsen, L.J., and K. Keegstra. 1992. The binding of precursor proteins to chloroplasts requires nucleoside triphosphates in the intermembrane space. J. Biol. Chem. 267:433-439.
25. Pain, D., and G. Blobel. 1987. Protein import in chloroplasts requires a chloroplast ATPase. Proc. Natl. Acad. Sci. USA. 84:3288-3292.
26. Perry, S.E., and K. Keegstra. 1994. Envelope membrane proteins that interact with chloroplastic precursor proteins. Plant Cell. 6:93-105.
27. Schnell, D.J., and G. Blobel. 1993. Identification of intermediates in the pathway of protein import into chloroplasts and their localization to envelope contact sites. J. Cell Biol. 120:103-115.
28. Schnell, D.J., G. Blobel, K. Keegstra, F. Kessler, K. Ko, and J. Soll. 1997. A nomenclature for the protein import components of the chloroplast envelope. Trends Cell Biol. 7:303-304.
29. Schnell, D.J., G. Blobel, and D. Pain. 1990. The chloroplast import receptor is an integral membrane protein of chloroplast envelope contact sites. J. Cell Biol. 111:1825-1838.
30. Schnell, D.J., F. Kessler, and G. Blobel. 1994. Isolation of components of the chloroplast protein import machinery. Science. 266:1007-1012.
31. Seedorf, M., K. Waegemann, and J. Soll. 1995. A constituent of the chloroplast import complex represents a new type of GTP-binding protein. Plant J. 7:401-411.
32. Segui-Real, B., G. Kispal, R. Lill, and W. Neupert. 1993. Functional independence of the protein translocation machineries in mitochondrial outer and inner membranes: passage of preproteins through the intermembrane space. EMBO (Eur. Mol. Biol. Organ.) J. 12:2211-2218.
33. Tranel, P.J., J. Froehlich, A. Goyal, and K. Keegstra. 1995. A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway. EMBO (Eur. Mol. Biol. Organ.) J. 14:2436-2446.
34. von Heijne, G., and Y. Gavel. 1988. Topogenic signals in integral membrane proteins. Eur. J. Biochem. 174:671-678.
35. Waegemann, K., and J. Soll. 1991. Characterization of the protein import apparatus in isolated outer envelopes of chloroplasts. Plant J. 1:149-158.