The enhancing of a cysteine proteinase activity at acidic pH by protein engineering, the role of glutamic 50 in the enzyme mechanism of caricain

FEBS Letters

Volumn 437, Issue 1-2, 1998, Pages 91-96

  Ikeuchi, Yoshihide ^a   Katerelos, Nikolaos A ^a   Goodenough, Peter W ^a  

^a School of Plant Sciences   (United Kingdom)

Author keywords

Cysteine proteinase; Kinetically influential mutant; Site directed mutagenesis

Indexed keywords

ACID; CARICAIN; CYSTEINE PROTEINASE; GLUTAMIC ACID; MUTANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; GENE OVEREXPRESSION; GENETIC ENGINEERING; PH; PRIORITY JOURNAL; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

BINDING SITES; CYSTEINE ENDOPEPTIDASES; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; PLANT PROTEINS;

EID: 0032538544     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(98)01194-6     Document Type: Article

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