Rapid deconvolution of combinatorial libraries using HPLC fractionation

Tetrahedron

Volumn 54, Issue 16, 1998, Pages 4067-4076

  Griffey, Richard H ^a   An, Haoyun ^a   Cummins, Lendell L ^a   Gaus, Hans J ^a   Haly, Becky ^a   Herrmann, Rigo ^a   Dan Cook, P ^a  

^a ISIS PHARMACEUTICALS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNCTIONAL GROUP; ISIS 100302; ISIS 110753; ISIS 113067; PYRIDINE DERIVATIVE; UNCLASSIFIED DRUG;

ANTIBACTERIAL ACTIVITY; CONFERENCE PAPER; DRUG STRUCTURE; DRUG SYNTHESIS; ESCHERICHIA COLI; FRACTIONATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; STREPTOCOCCUS PYOGENES;

EID: 0032537025     PISSN: 00404020     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4020(98)00135-5     Document Type: Conference Paper

References (26)

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26. 4 colony forming units per well, and mixed with the compounds under investigation in a 96-well format. The plates are incubated at 37°C for 24 h, and optical density is read using a BioRad 3550 UV microplate reader. Concentrations of material in each fraction were estimated based on the weighted molecular masses of all library members. This calculation can introduce an error of ±10% in the actual concentration submitted for testing.