Probing the structure of complex macromolecular interactions by homolog specificity scanning: The P1 and P7 plasmid partition systems

EMBO Journal

Volumn 17, Issue 20, 1998, Pages 6076-6085

  Radnedge, Lyndsay ^b   Youngren, Brenda ^a   Davis, Michael ^c   Austin, Stuart ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^c CENTRAL CONNECTICUT STATE UNIVERSITY   (United States)

Author keywords

Homolog specificity scanning; Macromolecular interactions; Plasmid partition systems; Protein DNA; Protein protein interactions

Indexed keywords

ARTICLE; ESCHERICHIA COLI; MACROMOLECULE; MACRONUCLEUS; MOLECULAR INTERACTION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BACTERIOPHAGE P1; COLIPHAGES; DIMERIZATION; ESCHERICHIA COLI; MACROMOLECULAR SUBSTANCES; MOLECULAR SEQUENCE DATA; OPERON; PEPTIDE MAPPING; PHYSICAL CHROMOSOME MAPPING; PLASMIDS; PROMOTER REGIONS (GENETICS); REPRESSOR PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY;

EID: 0032531743     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.20.6076     Document Type: Article

References (22)

1. Abeles,A.L., Friedman,S.A. and Austin,S.J. (1985) Partition of unit-copy miniplasmids to daughter cells III. The DNA sequence and functional organization of the P1 partition region. J. Mol. Biol., 185, 261-272.
2. Bolivar,F., Rodriguez,R.L., Green,P.J., Betlach,M.D., Boyer,H.W., Crosa,J.H. and Falkow,S. (1977) Construction and characterization of new cloning vehicles. II: A multipurpose cloning system. Gene, 2, 95-113.
3. Churehward,G., Belin,D. and Nagamine,Y. (1984) A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene, 31, 165-171.
4. Cunningham,B.C., Jhurani,P., Ng,P. and Wells,J.A. (1989) Receptor and antibody epitopes in human growth hormone identified by homolog-scanning mutagenesis. Science, 243, 1330-1336.
5. Davey,M.J. and Funnell,B.E. (1994) The P1 plasmid partition protein ParA. J. Biol. Chem., 269, 29908-29913.
6. Davis,M.A. and Austin,S.J. (1988) Recognition of the P1 plasmid centromere analog involves binding of the ParB protein and is modified by a specific host factor. EMBO J., 7, 1881-1888.
7. Davis,M.A., Martin,K.A. and Austin,S.J. (1992) Biochemical activities of the ParA partition protein of the P1 plasmid. Mol. Microbiol., 6, 1141-1147.
8. Davis,M.A., Radnedge,L., Martin,K.A., Hayes,F., Youngren,B. and Austin,S.J. (1996) The P1 ParA protein and its ATPase activity play a direct role in the segregation of plasmid copies to daughter cells. Mol. Microbiol., 21, 1029-1036.
9. Friedman,S.A. and Austin,S.J. (1988) The P1 plasmid-partition system synthesizes two essential proteins from an autoregulated operon. Plasmid, 19, 103-112.
10. Funnell,B.E. (1988) Participation of Escherichia coli integration host factor in the PI plasmid partition system. Proc. Natl Acad. Sci. USA, 85, 6657-6661.
11. Hayes,F. and Austin,S.J. (1993) Specificity determinants of the P1 and P7 plasmid centromere analogs. Proc. Natl Acad. Sci. USA, 90, 9228-9232.
12. Hayes,F., Radnedge,L., Davis,M.A. and Austin,S.J. (1994) The homologous operons for P1 and P7 plasmid partition are autoregulated from dissimilar operator sites. Mol. Microbiol., 11, 249-260.
13. Horton,R.M., Cai,Z., Ho,S.N. and Pease,L.R. (1990) Gene splicing by overlap extension. Biotechniques, 8, 528-535.
14. Lin,C.-H., Levin,P.A. and Grossman,A.D. (1997) Bipolar localization of a chromosome partition protein in Bacillus subtilis. Proc. Natl Acad. Sci. USA, 94, 4721-4726.
15. Lobocka,M. and Yarmolinsky,M. (1996) P1 plasmid partition: a mutational analysis of ParB. J. Mol. Biol., 25, 366-382.
16. Ludtke,D.N., Eichorn,B.G. and Austin,S.J. (1989) Plasmid-partition functions of the P7 prophage. J. Mol. Biol., 209, 393-406.
17. Mohl,D. A. and Gober,J.W. (1997) Cell-cycle dependent polar localization of chromosome partitioning proteins in Caulobacter crescentus. Cell, 88, 675-684.
18. Radnedge,L., Davis,M.A. and Austin,S.J. (1996) P1 and P7 plasmid partition: ParB protein bound to its partition site makes a separate discriminator contact with the DNA that determines species specificity. EMBO J., 15, 1155-1162.
19. Sambrook,J., Fritsch,E.F. and Maniatis,T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Webb,C.D., Teleman,A., Gordon,S., Straight,A., Belmont,A., Lin,D.C.-H., Grossman,A.D., Wright,A. and Losick,R. (1997) Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B.subtilis. Cell, 88, 667-674.
21. Youderian,P., Vershon,A., Bouvier,S., Sauer,R. and Susskind,M. (1983) Changing the DNA-binding specificity of a repressoc Cell, 35, 777-783.
22. Youngren,B. and Austin,S. (1997) Altered ParA partition proteins of plasmid P1 act via the partition site to block plasmid propagation. Mol. Microbiol., 25, 1023-1030.