Altered ParA partition proteins of plasmid P1 act via the partition site to block plasmid propagation

Molecular Microbiology

Volumn 25, Issue 6, 1997, Pages 1023-1030

  Youngren, Brenda ^a   Austin, Stuart ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN;

ARTICLE; BACTERIOPHAGE LAMBDA; MUTANT; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; VIRUS CULTURE;

BACTERIOPHAGE LAMBDA;

EID: 0030955388     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.4761842.x     Document Type: Article

References (22)

1. Abeles, A.L., Friedman, S.A., and Austin, S.J. (1985) Partition of unit-copy miniplasmids to daughter cells III. The DNA sequence and functional organization of the P1 partition region. J Mol Biol 185: 261-272.
2. Abeles, A.L., Reaves, L.D., Youngren-Grimes, B., and Austin, S.J. (1995) Control of P1 plasmid replication by iterons. Mol Microbiol 18: 903-912.
3. Austin, S., and Abeles, A. (1983) Partition of unit-copy miniplasmids to daughter cells II. The partition region of miniplasmid P1 encodes an essential protein and a centromere-like site at which it acts. J Mol Biol 169: 373-387.
4. Austin, S.J., and Eichorn, B.G. (1992) Random diffusion can account for top4-dependent suppression of partition defects in low-copy-number plasmids. J Bacteriol 174: 5190-5195.
5. Austin, S.J., and Nordström, K. (1990) Partition-mediated incompatibility of bacterial plasmids. Cell 60: 351-354.
6. Austin, S., Ziese, M., and Sternberg, N. (1981) A novel role for site-specific recombination in maintenance of bacterial replicons. Cell 25: 729-736.
7. Bolivar, F., Rodriguez, R.L., Green, P.J., Betlach, M.D., Boyer, H.W., Crosa, J.H., and Falkow, S. (1977) Construction and characterization of new cloning vehicles. II: A multipurpose cloning system. Gene 2: 95-113.
8. Das, A., Court, D., and Adhya, S. (1976) Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription and termination of factor rho. Proc Natl Acad Sci USA 73: 1959-1963.
9. Davis, M.A., and Austin, S.J. (1988) Recognition of the P1 plasmid centromere analog involves binding of the ParB protein and is modified by a specific host factor. EMBO J 7: 1881-1888.
10. Davis, M.A., Martin, K.A., and Austin, S.J. (1992) Biochemical activities of the ParA partition protein of the P1 plasmid. Mol Microbiol 6: 1141-1147.
11. Davis, M.A., Radnedge, L., Martin, K.A., Hayes, F., Youngren, B., and Austin, S.J. (1996) The P1 ParA protein and its ATPase activity play a direct role in the segregation of plasmid copies to daughter cells. Mol Microbiol 21: 1029-1036.
12. Friedman, S.A., and Austin, S.J. (1988) The P1 plasmid-partition system synthesizes two essential proteins from an autoregulated operon. Plasmid 19: 103-112.
13. Funnell, B.E. (1988a) Mini-P1 plasmid partitioning: excess ParB protein destabilizes plasmids containing the centromere parS. J Bacteriol 170: 954-960.
14. Funnell, B.E. (1988b) Participation of Escherichia coli integration host factor in the P1 plasmid partition system. Proc Natl Acad Sci USA 85: 6657-6661.
15. Hayes, F., and Austin, S.J. (1993) Specificity determinants of the P1 and P7 plasmid centromere analogs. Proc Natl Acad Sci USA 90: 9228-9232.
16. Hayes, F., Davis, M.A., and Austin, S.J. (1993) Fine-structure analysis of the P7 plasmid partition site. J Bacteriol 175: 3443-3451.
17. Hayes, F., Radnedge, L., Davis, M.A., and Austin, S.J. (1994) The homologous operons for P1 and P7 plasmid partition are autoregulated from dissimilar operator sites. Mol Microbiol 11: 249-260.
18. Lobocka, M., and Yarmolinsky, M. (1996) P1 plasmid partition: a mutational analysis of ParB. J Mol Biol 259: 366-382.
19. Nordström, K., and Austin, S.J. (1989) Mechanisms that contribute to the stable segregation of plasmids. Annu Rev Genet 23: 37-69.
20. Radnedge, L., Davis, M.A., and Austin, S.J. (1996) P1 and P7 plasmid partition: ParB protein bound to its partition site makes a separate discriminator contact with the DNA that determines species specificity. EMBO J 15: 1155-1162.
21. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
22. Sternberg, N., and Austin, S. (1983) Isolation and characterization of P1 minireplicons, λ-P1:5R and λ-P1: 5L. J Bacteriol 153: 800-812.