Determination of anandamide amidase activity using ultraviolet-active amine derivatives and reverse-phase high-performance liquid chromatography

Analytical Biochemistry

Volumn 261, Issue 1, 1998, Pages 8-15

  Qin, Ce ^a   Lin, Sonyuan ^a   Lang, Wensheng ^a   Goutopoulos, Andreas ^a   Pavlopoulos, Spiro ^a   Mauri, Frank ^a   Makriyannis, Alexandros ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ETHANOLAMINES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SOLVENT EXTRACTION; UNSATURATED FATTY ACIDS;

AMINE DERIVATIVES; ARACHIDONIC ACIDS; ENZYME SUBSTRATES; EXTRACTION PROCEDURE; HYDROLYSIS PRODUCTS; O-PHTHALDIALDEHYDE; PRECOLUMN DERIVATIZATIONS; REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ENZYMATIC HYDROLYSIS;

AMIDASE; ANANDAMIDE; ARACHIDONIC ACID; AMIDE; ARACHIDONIC ACID DERIVATIVE; ARACHIDONOYLETHANOLAMIDE SYNTHASE; ENZYME INHIBITOR; ETHANOLAMINE; LIGAND; METHANANDAMIDE;

ANIMAL TISSUE; ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; RAT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAVIOLET RADIATION; ANIMAL; BRAIN; CHEMISTRY; DRUG ANTAGONISM; ENZYME SPECIFICITY; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; METABOLISM; METHODOLOGY; MICROSOME; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; STATISTICS; ULTRAVIOLET SPECTROPHOTOMETRY;

ANIMALIA;

AMIDOHYDROLASES; ANIMALS; ARACHIDONIC ACID; ARACHIDONIC ACIDS; BRAIN; CHEMISTRY, ANALYTICAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME INHIBITORS; ETHANOLAMINE; LIGANDS; MICROSOMES; POLYUNSATURATED ALKAMIDES; RATS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY, ULTRAVIOLET; SUBSTRATE SPECIFICITY;

EID: 0032528535     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1998.2713     Document Type: Article

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