The molecular chaperone Ssb from Saccharomyces cerevisiae is a component of the ribosome-nascent chain complex

EMBO Journal

Volumn 17, Issue 14, 1998, Pages 3981-3989

  Pfund, Christine ^a   Lopez Hoyo, Nelson ^b   Ziegelhoffer, Thomas ^a   Schilke, Brenda A ^a   Lopez Buesa, Pascual ^a   Walter, William A ^a   Wiedmann, Martin ^c   Craig, Elizabeth A ^d  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^d NONE

Author keywords

Chaperone; Protein folding; Ribosome nascent chain complex; Ribosomes; Ssb

Indexed keywords

AMINOACYL TRANSFER RNA; CHAPERONE; FUNGAL PROTEIN; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 70; LYSINE; PUROMYCIN; RIBOSOME PROTEIN;

ARTICLE; CONTROLLED STUDY; CROSS LINKING; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN SECRETION; RIBOSOME; SACCHAROMYCES CEREVISIAE;

ADENOSINE TRIPHOSPHATE; CROSS-LINKING REAGENTS; FUNGAL PROTEINS; HSP70 HEAT-SHOCK PROTEINS; LIGHT; LITHIUM CHLORIDE; POTASSIUM CHLORIDE; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; PROTEIN FOLDING; PUROMYCIN; RIBOSOMES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TEMPERATURE;

EID: 0032528301     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.14.3981     Document Type: Article

References (57)

1. Banecki, B. and Zylicz, M. (1996) Real time kinetics of the DnaK/DnaJ/GrpE molecular chaperone machine action. J. Biol. Chem., 271, 6137-6143.
2. Beckmann, R.P., Mizzen, L. and Welch,W. (1990) Interaction of Hsp70 with newly synthesized proteins: implications for protein folding and assembly. Science, 248, 850-856.
3. Beckmann, R., Bubeck, D., Grassucci, R., Penczek, R., Verchoor, A., Blobel, G. and Frank, J. (1997) Alignment of conduits for nascent polypeptide chain in the ribosome-Sec61 complex. Science, 278, 2123-2126.
4. Bernabeu, C., Tobin, E.M., Fowler, A., Jabin, I. and Lake, J.A. (1983) Nascent polypeptide chains exit the ribosorne in the same relative position in both eucaryotes and procaryotes. J. Cell Biol., 96, 1471-1474.
5. Blom, J., Kubrich, M., Rassow, J., Voos, W., Dekker, P.J.T., Maarse, A.C., Meijer, M. and Pfanner, K. (1993) The essential yeast protein MIM44 (encoded by MPII) is involved in an early step of preprotein translocation across the mitochondria inner membrane. Mol. Cell. Biol., 13, 7364-7371.
6. Blond-Elguindi, S., Cwirla, S., Dower, W., Lipshutz, R., Sprang, S., Sambrook, J. and Gething, M.J. (1993) Affinity panning of a library of peptides displayed on bacteriophages reveals the binding specificity of BiP. Cell, 75, 717-728.
7. Boorstein, W.R., Ziegelhoffer, T. and Craig, E.A. (1994) Molecular evolution of the HSP70 multigene family. J. Mol. Evol. 38, 1-17.
8. Chappell, T.G., Konforti, B.B., Schmid, S.L. and Rothman, J.E. (1987) The ATPase core of a clathrin uncoating protein. J. Biol. Chem., 262, 746-751.
9. Chirico, W., Waters, M.G. and Blobel, G. (1988) 70K heat shock related proteins stimulate protein translocation into microsomes. Nature, 332, 805-810.
10. Craig, E.A. and Jacobsen, K. (1985) Mutations in cognate gene of Saccharomyces cerevisiae HSP70 result in reduced growth rates at low temperatures. J. Biol. Chem., 5, 3517-3524.
11. Craig, E.A., Gambill, B.D. and Nelson, R.J. (1993) Heat shock proteins: molecular chaperones of protein biogenesis. Microbiol. Rev., 57, 402-414.
12. Craig, E.A., Ziegelhoffer, T., Nelson, J., Laloray, S. and Halladay, J. (1995) Complex multigene family of functionally distinct Hsp70s of yeast. Cold Spring Harbor Symp. Quant. Biol., 60, pp. 441-449.
13. Cyr, D.M. and Douglas, M.G. (1994) Differential regulation of Hsp70 subfamilies by the eukaryotic DnaJ homologue YDJ1. J. Biol. Chem., 269, 9798-9804.
14. Deshaies, R., Koch, B., Werner-Washburne, M., Craig, E. and Schekman, R. (1988) A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypcptides. Nature, 332, 800-805.
15. Eggers, D.K., Welch, W.J. and Hansen,W.J. (1997) Complexes between nascent polypeptides and their molecular chaperones in the cytosol of mammalian cells. Mol. Biol. Cell, 8, 1559-1573.
16. El-Baradi, T.T., Raue, H.A., Regt, C.H.D. and Planta, R.J. (1984) Stepwise dissociation of yeast 60S ribosomal subunits by LiCl and identification of L25 as a primary 26S rRNA binding protein. Eur. J. Biochem., 144, 393-400.
17. Erickson, A.H. and Blobel, G. (1983) Cell-free translation of messenger RNA in a wheat germ system. Methods Enzymol., 96, 38-50.
18. Flaherty, K.M., DeLuca-Flaherty, C. and McKay, D.B. (1990) Three dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein. Nature, 346, 623-628.
19. Flynn, G.C., Chappell, T.G. and Rothman, J.E. (1989) Peptide binding and release by proteins implicated as catalysts of protein assembly. Science, 245, 385-390.
20. Frydman, J., Nimmesgern, E., Ohtsuka, K. and Hartl, F.U. (1994) Folding of nascent polypeptide chains in a high molecular mass assembly with molecular chaperones. Nature, 370, 111-117.
21. Gilmore, R., Collins, P., Johnson, J., Kellaris, K. and Rapiejko, P. (1991) Transcription of full-length and truncated mRNA transcripts to study protein translocation across the endoplasmic reticulum. Methods Cell Biol., 34, 223-239.
22. Gorlich, D., Kurzchalia, T.V., Wiedmann, M. and Rapoport, T.A. (1991) Probing the molecular environment of translocating polypeptide chains by cross-linking. Methods Cell Biol., 34, 241-262.
23. Gragerov, A., Zeng, L., Zhao, X., Burkholder, W. and Gottesman, M.E. (1994) Specificity of DnaK-peptide binding. J. Mol. Biol., 235, 848-854.
24. Greene, L., Zinner, R., Naficy, S. and Eisenberg, E. (1995) Effect of nuclcotide on the binding of peptides to 70-kDa heat shock proteins. J. Biol. Chem., 270, 2967-2973.
25. Hartl, F.U. (1996) Molecular chaperones in cellular protein folding. Nature, 381, 571-580.
26. Hurtwell, L.H. and McLaughlin, C.S. (1968) A mutant of yeast apparently defective in the initiation of protein synthesis. Proc. Natl Acad. Sci. USA, 62, 468-474.
27. Haus, U., Trommler, P., Fisher, P.R., Hartmann, H., Lottspeich, F., Noegel, A.A. and Schleicher, M. (1993) The heal shock cognate protein from Dictyostelium affects actin polymerization through interaction with the actin-binding protein cap32/34. EMBO J., 12, 3763-3771.
28. Hendrick, J.P., Langer, T., Davis J.A., Hartl, F.U. and Wiedmann, M. (1993) Control of folding and membrane translocation by binding of the chaperone DnaJ to nascent polypeptides. Proc. Natl Acad. Sci. USA, 90, 10216-10220.
29. Hesterkamp, T., Hauser, S., Henrich, L. and Bukau, B. (1996) Escherichia coli trigger factor is a prolyl isomerase that associates with nascent polypeptide chains. Proc. Natl Acad. Sci. USA, 93, 4437-4441.
30. Huang, S.P., Tsai, M.-Y., Tzou, Y.-M., Wu, W.G. and Wang, C. (1993) Aspartyl residue 10 is essential for ATPase activity of rat hsc70. J. Biol. Chem., 268, 2063-2068.
31. James, P., Pfund, C. and Craig, E. (1997) Functional specificity among Hsp70 molecular chaperones. Science, 275, 387-389.
32. Kudlicki, W., Chirgwin, J., Kramer, G. and Hardesty, B. (1995) Folding of an enzyme into an active conformation while bound as pepridyl-tRNA to the ribosome. Biochemistry, 34, 14284-14287.
33. Kurzchalia, T.V., Wiedmann, M., Girshovich, A.S., Bochkareva, E.S., Bielka, H. and Rapoport, T.A. (1986) The signal sequence of nascent preprolactin interacts with 54K polypeptide of the signal recognition particle. Nature, 320, 634-636.
34. Launng, B., Sakai, H., Kreibich, G. and Wiedmann, M. (1995) Nascent polypeptide-associated complex protein prevents mistargeting of nascent chains to the endoplasmic reticulum. Proc. Natl Acad. Sci. USA, 92, 5411-5415.
35. Lyman, S.K. and Schekman, R. (1995) Interaction between BiP and Sec63p is required for the completion of protein translocation into the ER of Saccharomyces cerevisiae. J. Cell. Biol., 131, 1163-1171.
36. McCarty, J., Buchberger, A., Reinstein, J. and Bukau, B. (1995) The role of ATP in the functional cycle of the DnaK chaperone system. J. Mol. Biol., 249, 126-137.
37. Milarski, K.L. and Morimoto, R.I. (1989) Mutational analysis of the human hsp70 protein: distinct domains for nucleolar localization and adenosine triphosphate binding. J. Cell. Biol., 109, 1947-1962.
38. Morimoto, R.I., Tissieres, A. and Georgopoulos, C. (1994) The Biology of Heat Shock Proteins and Molecular Chaperones. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
39. Murakami, H., Pain, D. and Blobel, G. (1988) 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. J. Cell. Biol., 107, 2051-2057.
40. Musch, A., Wiedmann, M. and Rapoprt, T.A. (1992) Yeast Sec proteins interact with polypeptides transversing the endoplasmic reticulum membrane. Cell, 69, 343-352.
41. Nelson, R.J., Ziegelhoffer, T., Nicolet, C., Werner-Washburne, M. and Craig, E.A. (1992) The translation machinery and seventy kilodalton hear shock protein cooperate in protein synthesis. Cell, 71, 97-105.
42. Ogg, S.C. and Walter, P. (1995) SRP samples nascent chains for the presence of signal sequence by interacting with ribosomes at a discrete step during translation elongation. Cell, 81, 1075-1084.
43. + but not ATP hydrolysis. Nature, 365, 664-666.
44. Powers, T. and Walter, P. (1996) The nascent polypeptide-associated complex modulates interactions between the signal recognition particle and the ribosome. Curr. Biol., 6, 331-338.
45. Sadler, I., Chiang, A., Kurihara, T., Rothblatt, J., Way, J. and Silver, P. (1989) A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein. J. Cell. Biol., 109, 2665-2675.
46. Schmid, D., Baici, A., Gehring, H. and Christen, P. (1994) Kinetics of molecular chaperone action. Science.,263, 971-973.
47. Schneider, H.C., Berthold, J., Bauer, M.F., Dietmeier, K., Guiard, B., Brunner, M. and Neupert, W. (1994) Mitochondrial Hsp70/MIM44 complex facilitates protein import. Nature, 371, 768-774.
48. Thiele, D., Cottrelle, P., Iborra, F., Buhler, J.M., Sentenac, A. and Fromageot, P. (1985) Elongation factor 1α from Saccharomyces centvisiae. J. Biol. Chem., 260, 3084-3089.
49. Tsui, M.Y. and Wang, C. (1994) Uncoupling of peptide-stimulated ATPase and clathrin-uncoating activity in deletion mutant of hsc70. J. Biol. Chem. 269, 5958-5962.
50. Valent, Q.A., Kendall, D.A., High, S., Kusters, R., Oudega, B. and Luirink, J. (1995) Early events in preprotein recognition in E.coli: interaction of SRP and trigger factor with nascent polypeptides. EMBO J., 14, 5494-5505.
51. Verschoor, A., Srivastava, S., Grassucci, R. and Frank, J. (1996) Native 3D structure of eukaryotic 80S ribosome: morphological homology with the E.coli 70S ribosome. J. Cell. Biol., 133, 495-505.
52. Wang, S., Sakai, H. and Wiedmann, M. (1995) NAC covers ribosome-associated nascent chains thereby forming a protective environment for regions of nascent chains just emerging from the peptidyl transferase center. J. Cell. Biol., 130, 519-528.
53. Wang, T.F., Chang, J.H. and Wang, C. (1993) Identification of the peptide binding domain of hsc70. J. Biol. Chem., 268, 26049-26051.
54. Waters, M.G. and Blobel, G. (1986) Secretory protein translocation in a yeast cell-free system can occur post-translationally and requires ATP hydrolysis. J. Cell. Biol., 102, 1543-1550.
55. Wiedmann, M., Kurzchalia, T.V., Bielka, H. and Rapoport, J.A. (1987) Direct probing of the interaction between the signal sequence of nascent preprolcatin and the signal recognition particle by specific cross-linking. J. Cell. Biol., 104, 201-208.
56. Wiedmann, B., Sakai, H., Davis, T. and Wiedmann, M. (1994) A protein complex required for signal-sequence-specific sorting and translation. Nature, 370, 434-440.
57. Zhu, X., Zhao, X., Burkholder, W., Gragerov, A., Ogata, C., Gottesman, M. and Hendrickson, W. (1996) Structural analysis of substrate binding by the molecular chaperone DnaK. Science, 272, 1606-1614.