GTP-binding-protein-coupled receptor kinase 2 (GRK2) binds and phosphorylates tubulin

European Journal of Biochemistry

Volumn 255, Issue 2, 1998, Pages 363-368

  Haga, Kazuko ^a   Ogawa, Haruo ^a   Haga, Tatsuya ^a   Murofushi, Hiromu ^b  

^a UNIVERSITY OF TOKYO   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

G protein coupled receptor; G protein coupled receptor kinase; Muscarinic acetylcholine receptor; Protein phosphorylation; Tubulin

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; MUSCARINIC RECEPTOR; PROTEIN KINASE; TUBULIN;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BINDING SITE; BRAIN; CONTROLLED STUDY; ENZYME BINDING; IMMUNOBLOTTING; IN VITRO STUDY; MICROTUBULE ASSEMBLY; MOLECULAR WEIGHT; MUTANT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SWINE;

ANIMALS; ATROPINE; BETA-ADRENERGIC RECEPTOR KINASE; BINDING SITES; BRAIN; CARBACHOL; CYCLIC AMP-DEPENDENT PROTEIN KINASES; GLUTATHIONE TRANSFERASE; GTP-BINDING PROTEINS; HEPARIN; KINETICS; MACROMOLECULAR SUBSTANCES; PHOSPHORYLATION; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SWINE; TUBULIN;

EID: 0032528248     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2550363.x     Document Type: Article

References (26)

1. Palezewski, K. & Benovic, J. L. (1991) G-protein-coupled receptor kinases. Trends Biochem. Sci. 16, 387-391.
2. Haga, T., Haga, K. & Kameyama, K. (1994) G protein-coupled receptor kinases, J. Neurochem. 63, 400-412.
3. Premont, R. T., Inglese, J. & Lefkowitz., R. J. (1995) Protein kinases that phosphorylate activated G protein-coupled receptors, FASEB J. 9, 175-182.
4. Kameyama, K., Haga, K., Haga, T., Moro, O. & Sadée, W. (1994) Activation of a GTP-binding protein and a GTP-binding-protein-coupled receptor kinase (β-adrenergic-receptor kinase-1) by a muscarinic receptor m2 mutant lacking phosphorylation sites, Eur. J. Biochem. 226, 267-276.
5. Haga, K., Kameyama, K. & Haga, T. (1994) Synergistic activation of a G protein-coupled receptor kinase by G protein beta gamma subunits and mastoparan or related peptides, J. Biol. Chem. 209, 12594-12599.
6. Nakata, H., Kameyama, K., Haga, K. & Haga, T. (1994) Location of agonist-dependent-phosphorylation sites in the third intracellular loop of muscarinic acetylcholine receptors (m2 subtype), Eur. J. Biochem. 220, 29-36.
7. Kameyama, K., Haga, K., Haga, T., Kotani, K., Katada, T. & Fukada, Y. (1993) Activation by G protein beta-gamma subunits of beta adrenergic and muscarinic receptor kinase, J. Biol. Chem. 268, 7753-7758.
8. Koch, W. J., Inglese, J., Stone, W. C. & Lefkowitz, R. J. (1993) The binding site for the beta gamma subunits of heterotrimeric G proteins on the beta-adrenergic receptor kinase, J. Biol. Chem. 268, 8256-8260.
9. β subunits and lipid, J. Biol. Chem. 270, 11707-11710.
10. Debburman, S. K., Ptasienski, J., Benovic, J. L. & Hosey, M. M. (1996) G protein-coupled receptor kinase GRK2 is a phospholipid-dependent enzyme that can be conditionally activated by G protein βγ subunits, J. Biol. Chem. 271, 22552-22562.
11. 2+/calmodulin, J. Biol. Chem. 271, 28691-28696.
12. 2+-dependent inhibition of G protein-coupled receptor kinase 2 by calmodulin, Biochemistiy 36, 1315-1321.
13. Shelanski, M. L., Gaskin, F. & Cantor, C. R. (1973) Microtubule assembly in the absence of added nucleotide, Proc. Natl Acad. Sci. USA 70, 705-768.
14. Weingarten, M. D., Lockwood, A. H., Hwo, S. Y. & Kirschner, M. W. (1975) A protein factor essential for microtubule assembly, Proc. Natl Acad. Sci. USA 72, 1858-1862.
15. Krauhs, E., Little, M., Kempf, T., Hofer-Warbinck, R., Ade, W. & Ponstingl, H. (1981) Complete amino acid sequence of beta-tubulin from porcine brain, Proc. Natl Acad. Sci. USA 78, 4156-4160.
16. Kumagai, H. & Sakai, H. (1983) A porcine brain protein (35 K protein) which bundles microtubules and its identification as glyceraldehyde 3-phosphate dehydrogenase, J. Biochem. 93, 1259-1269.
17. Benovic, J. L., Stone, W. C., Caron, M. G. & Lefkowitz, R. J. (1989) Inhibition of the beta-adrenergic receptor kinase by polyanions. J. Biol. Chem. 264, 6707-6710.
18. Timasheff, S. N. & Grisham, L. M. (1980) In vitro assembly of cytoplasmic microtubules, Annu. Rev. Biochem. 49, 565-591.
19. Eiper, B. A. (1972) Rat brain microtubule protein: purification and determination of covalently bound phosphate and carbohydrate, Proc. Natl Acad. Sci. USA 69, 2283-2287.
20. Sloboda, R. D., Rudolph, S. A., Rosenbaum, J. L. & Greengard, P. (1975) Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein, Proc. Natl Acad. Sci. USA 72, 177-181.
21. 2+- and calmodulin-stimulated endogenous phosphorylation of neurotubulin, Proc. Natl Acad. Sci. USA 78, 991-995.
22. Kadowaki, T., Fujita-Yamaguchi, Y., Nishida, E., Takaku, F., Akiyama, T., Kathuria, S., Akanuma, Y. & Kasuga, M. (1985) Phosphorylation of tubulin and microtubule-associated proteins by the purified insulin receptor kinase, J. Biol. Chem. 260, 4016-4020.
23. Yamauchi, T. & Fujisawa, H. (1983) Disassembly of microtubules by the action of calmodulin-dependent protein kinase (Kinase II) which occurs only in the brain tissues, Biochem. Biophys. Res. Commun, 110, 287-291.
24. Hargreaves, A. J., Wandosell, F. & Avila, J. (1997) Phosphorylation of tubulin enhances its interaction with membranes. Nature 323, 827-828.
25. Pitcher, J. A., Inglese, J., Higgins, J. B., Arriza, J. L., Casey, P. J., Kim, C., Benovic, J. L., Kwatra, M. M., Caron, M. G. & Lefkowitz, R. J. (1992) Role of beta-gamma subunits of G proteins in targeting the beta-adrenergic receptor kinase to membrane-bound receptors, Science 257, 1264-1267.
26. Ozawa, F., Kasuya, Y, Hama, H., Asada, S., Inada, T. & Goto, K. (1997) Microtubule dynamics regulates the level of endothelin-B receptor in rat cultured astrocytes. J. Neurochem. 69, 562-569.