COI1: An Arabidopsis gene required for jasmonate-regulated defense and fertility

Science

Volumn 280, Issue 5366, 1998, Pages 1091-1094

  Xie, Dao Xin ^a   Feys, Bart F ^a,b   James, Sarah ^a,c   Nieto Rostro, Manuela ^a   Turner, John G ^a  

^a UNIVERSITY OF EAST ANGLIA   (United Kingdom)

^b SAINSBURY LABORATORY   (United Kingdom)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

JASMONIC ACID;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENETIC ANALYSIS; NUCLEOTIDE SEQUENCE; PREDICTION; PRIORITY JOURNAL; PROTEIN TARGETING; REPRESSOR GENE; SEQUENCE ANALYSIS; WOUND HEALING;

ACETIC ACIDS; AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CHROMOSOME MAPPING; CYCLOPENTANES; GENES, PLANT; GENETIC COMPLEMENTATION TEST; MOLECULAR SEQUENCE DATA; MUTATION; OPEN READING FRAMES; PLANT GROWTH REGULATORS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; POLYMORPHISM, GENETIC; REPRESSOR PROTEINS; SIGNAL TRANSDUCTION; TRANSFORMATION, GENETIC; UBIQUITINS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; EMBRYOPHYTA; HEXAPODA;

EID: 0032524404     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5366.1091     Document Type: Article

References (41)

1. G. Sembdner and B. Parthier, Annu. Rev. Plant Physiol. 44, 569 (1993); R. A. Creelman and J. M. Mullet, Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 355 (1997).
2. G. Sembdner and B. Parthier, Annu. Rev. Plant Physiol. 44, 569 (1993); R. A. Creelman and J. M. Mullet, Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 355 (1997).
3. C. L. Ryan, Plant Mol. Biol. 19, 123 (1992).
4. H. Pena-Cortes, T. Albrecht, S. Prat, E. W. Weiler, L. Willmitzer, Planta 191, 123 (1993); G. A. Howe, J. Lightner, J. Browse, C. A. Ryan, Plant Cell 8, 2067 (1996).
5. H. Pena-Cortes, T. Albrecht, S. Prat, E. W. Weiler, L. Willmitzer, Planta 191, 123 (1993); G. A. Howe, J. Lightner, J. Browse, C. A. Ryan, Plant Cell 8, 2067 (1996).
6. P. J. O'Donnell et al., Science 274, 1914 (1996).
7. M. McConn and J. Browse, Plant Cell 8, 403 (1996).
8. B. F. Feys, C. E. Benedetti, C. N. Penfold, J. Turner, ibid. 6, 751 (1994).
9. P. E. Staswick, W. Su, S. H. Howell, Proc. Natl. Acad. Sci. U.S.A. 89, 6837 (1992); S. Berger, E. Bell, J. E. Mullet, Plant Physiol. 111, 525 (1996).
10. P. E. Staswick, W. Su, S. H. Howell, Proc. Natl. Acad. Sci. U.S.A. 89, 6837 (1992); S. Berger, E. Bell, J. E. Mullet, Plant Physiol. 111, 525 (1996).
11. M. McConn, R. A. Creelman, E. Bell, J. Mullet, J. Browse, Proc. Natl. Acad. Sci. U.S.A. 94, 5473 (1997).
12. E. Rojo et al., Plant J. 13, 153 (1998).
13. D.-X. Xie, B. F. Feys, S. James, M. Nieto-Rostro, J. G. Turner, unpublished data.
14. I. A. M. A. Penninckx et al., Plant Cell 8, 2309 (1996).
15. coi1 mutants representing three independent alleles (coi1-1, coi1-15, and coi1-18) were selected as seedlings with green leaves and well-developed roots on MS medium (mineral salts and sucrose, 3% w/v, solidified with agar, 0.6% w/v) supplemented with 50 μM methyl jasmonate (6).
16. A. Konieczny and F. M. Ausubel, Plant J. 4, 403 (1993).
17. A fragment of Thi2.1 gene [C. E. Eppel, K. Apel, H. Bohlman, Plant Physiol. 109, 1813 (1995)] was used as a probe to identify hybridizing clones in an Arabidopsis thaliana ecotype Columbia genomic library. From one clone, a fragment of ∼1 kb containing the Thi2.1 promoter was amplified by the polymerase chain reaction (PCR) with primers that hybridized to vector sequences and to sequences 5′ from the ATG start of Thi2.1. This fragment was fused to GUS at the Sma I site of pBI101.3 [R. A. Jefferson, T. A. Kavanagh, M. Bevan, EMBO J. 6, 3901 (1987)] to give PThi2.1-GUS. PThi2.1-GUS was then introduced into plants by Agrobacterium tumefaciens-mediated transformation (76). GUS activity was detected histochemically [R. A. Jefferson, Plant Mol. Biol. Rep. 5, 387 (1987)].
18. A fragment of Thi2.1 gene [C. E. Eppel, K. Apel, H. Bohlman, Plant Physiol. 109, 1813 (1995)] was used as a probe to identify hybridizing clones in an Arabidopsis thaliana ecotype Columbia genomic library. From one clone, a fragment of ∼1 kb containing the Thi2.1 promoter was amplified by the polymerase chain reaction (PCR) with primers that hybridized to vector sequences and to sequences 5′ from the ATG start of Thi2.1. This fragment was fused to GUS at the Sma I site of pBI101.3 [R. A. Jefferson, T. A. Kavanagh, M. Bevan, EMBO J. 6, 3901 (1987)] to give PThi2.1-GUS. PThi2.1-GUS was then introduced into plants by Agrobacterium tumefaciens-mediated transformation (76). GUS activity was detected histochemically [R. A. Jefferson, Plant Mol. Biol. Rep. 5, 387 (1987)].
19. A fragment of Thi2.1 gene [C. E. Eppel, K. Apel, H. Bohlman, Plant Physiol. 109, 1813 (1995)] was used as a probe to identify hybridizing clones in an Arabidopsis thaliana ecotype Columbia genomic library. From one clone, a fragment of ∼1 kb containing the Thi2.1 promoter was amplified by the polymerase chain reaction (PCR) with primers that hybridized to vector sequences and to sequences 5′ from the ATG start of Thi2.1. This fragment was fused to GUS at the Sma I site of pBI101.3 [R. A. Jefferson, T. A. Kavanagh, M. Bevan, EMBO J. 6, 3901 (1987)] to give PThi2.1-GUS. PThi2.1-GUS was then introduced into plants by Agrobacterium tumefaciens-mediated transformation (76). GUS activity was detected histochemically [R. A. Jefferson, Plant Mol. Biol. Rep. 5, 387 (1987)].
20. M. Mindrinos, F. Katagiri, G.-L. Yu, F. M. Ausubel, Cell 78, 1089 (1994).
21. Fragment 8ks (Fig. 1B) and Hind III-Sac I fragments of cDNAs 1.8c and 1.9c (Fig. 1C), containing the CaMV 35S promoter and full-length coding sequence, were each cloned into pBin19 [M. Bevan, Nucleic Acids Res. 12, 8711 (1984)] and then mobilized into Agrobacterium tumefaciens pGV3101 by electroporation. Flower buds of wild-type and COI1/ coi1-1 plants were transformed by in planta vacuum infiltration [A. Bent et al., Science 265, 1856 (1994)]. Transgenic plants were selected as seedlings with green leaves and well-developed roots on MS containing kanamycin (50 μg/ml) and confirmed by Southern (DNA) blot analysis.
22. Fragment 8ks (Fig. 1B) and Hind III-Sac I fragments of cDNAs 1.8c and 1.9c (Fig. 1C), containing the CaMV 35S promoter and full-length coding sequence, were each cloned into pBin19 [M. Bevan, Nucleic Acids Res. 12, 8711 (1984)] and then mobilized into Agrobacterium tumefaciens pGV3101 by electroporation. Flower buds of wild-type and COI1/ coi1-1 plants were transformed by in planta vacuum infiltration [A. Bent et al., Science 265, 1856 (1994)]. Transgenic plants were selected as seedlings with green leaves and well-developed roots on MS containing kanamycin (50 μg/ml) and confirmed by Southern (DNA) blot analysis.
23. D. Valvekens, M. Van Montagu, M. van Lijsebetten, Proc. Natl. Acad. Sci. U.S.A. 85, 5536 (1988).
24. Templates for DNA sequencing were overlapping subclones of fragments 8ks, 1.8c, and 1.9c (Fig. 1C) and fragments amplified from PCR from coi1 mutants (72) using Pfu DNA polymerase (Stratagene).
25. C. Bai et al., Cell 86, 263 (1996).
26. B. Kobe and J. Deisenhofer, Trends Biochem. Sci. 19, 415 (1994); S. G. St. C. Buchanan and N. J. Gay, Prog. Biophys. Mol. Biol. 65, 1 (1996).
27. B. Kobe and J. Deisenhofer, Trends Biochem. Sci. 19, 415 (1994); S. G. St. C. Buchanan and N. J. Gay, Prog. Biophys. Mol. Biol. 65, 1 (1996).
28. F. N. Li and M. Johnston, EMBO J. 16, 5629 (1997); D. Skowyra, K. L. Craig, M. Tyers, S. J. Elledge, J. W. Harper, Cell 91, 209 (1997).
29. F. N. Li and M. Johnston, EMBO J. 16, 5629 (1997); D. Skowyra, K. L. Craig, M. Tyers, S. J. Elledge, J. W. Harper, Cell 91, 209 (1997).
30. M. Ruegger et al., Genes Dev. 12, 198 (1998).
31. H. Zhang, R. Kobayashi, K. Galaktionov, D. Beach, Cell 82, 915 (1995); J. S. Flick and M. Johnston, Mol. Cell. Biol. 11, 5101 (1991); Y. Barral, S. Jentsch, C. Mann, Genes Dev. 9, 399 (1995).
32. H. Zhang, R. Kobayashi, K. Galaktionov, D. Beach, Cell 82, 915 (1995); J. S. Flick and M. Johnston, Mol. Cell. Biol. 11, 5101 (1991); Y. Barral, S. Jentsch, C. Mann, Genes Dev. 9, 399 (1995).
33. H. Zhang, R. Kobayashi, K. Galaktionov, D. Beach, Cell 82, 915 (1995); J. S. Flick and M. Johnston, Mol. Cell. Biol. 11, 5101 (1991); Y. Barral, S. Jentsch, C. Mann, Genes Dev. 9, 399 (1995).
34. The CAPS marker B23 revealed a DNA polymorphism between ecotypes Columbia and Landsberg erecta when Pst I was used to digest a PCR fragment amplified with the primers 5′-CTGCTTTTTT-GTGAGGTTTTG-3′ and 5′-CTCCACTCGCTAAA-CATCTG-3′.
35. YAC protocols were according to R. Schmidt, G. Cnops, I. Bancroft, C. Dean, Aust. J. Plant Physiol. 19, 341 (1992). YAC libraries were F. Creusot et al., Plant J. 8, 763 (1995); J. Ecker, Methods 1, 186 (1990); E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991). The IGF BAC library is described at http://194.94.225.1/private_workgroups/pg_101/ bac.html.
36. YAC protocols were according to R. Schmidt, G. Cnops, I. Bancroft, C. Dean, Aust. J. Plant Physiol. 19, 341 (1992). YAC libraries were F. Creusot et al., Plant J. 8, 763 (1995); J. Ecker, Methods 1, 186 (1990); E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991). The IGF BAC library is described at http://194.94.225.1/private_workgroups/pg_101/ bac.html.
37. YAC protocols were according to R. Schmidt, G. Cnops, I. Bancroft, C. Dean, Aust. J. Plant Physiol. 19, 341 (1992). YAC libraries were F. Creusot et al., Plant J. 8, 763 (1995); J. Ecker, Methods 1, 186 (1990); E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991). The IGF BAC library is described at http://194.94.225.1/private_workgroups/pg_101/ bac.html.
38. YAC protocols were according to R. Schmidt, G. Cnops, I. Bancroft, C. Dean, Aust. J. Plant Physiol. 19, 341 (1992). YAC libraries were F. Creusot et al., Plant J. 8, 763 (1995); J. Ecker, Methods 1, 186 (1990); E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991). The IGF BAC library is described at http://194.94.225.1/private_workgroups/pg_101/ bac.html.
39. P. Christou and W. F. Swain, Trends Biotechnol. 8, 145 (1990).
40. Abbreviations for the amino acids are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; Y, Tyr; and *, stop codon.
41. We thank J. Tashpulatov and E. Uslu for technical assistance; F. M. Ausubel for the cDNA library; J. Chory for information on C83; R. Schmidt, C. Dean, and D. Bouchez for support in screening YAC libraries and providing YAC clones; M. Estelle and J. Sanchez-Serrano for sharing information before publication; Nottingham Arabidopsis Stock Centre for seed; and the Arabidopsis Biological Resource Centre for restriction fragment length polymorphism clones, DNA libraries, and BAC filters. Supported by grant G01325 from the BBSRC, UK.