Replacement of the active site tyrosine of vaccinia DNA topoisomerase by glutamate, cysteine or histidine converts the enzyme into a site-specific endonuclease

Nucleic Acids Research

Volumn 26, Issue 2, 1998, Pages 490-496

  Wittschieben, John ^a   Petersen, Birgitte Ø ^a   Shuman, Stewart ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; DNA TOPOISOMERASE; DOUBLE STRANDED DNA; GLUTAMIC ACID; HISTIDINE; RESTRICTION ENDONUCLEASE; TYROSINE;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL REACTION KINETICS; DNA ADDUCT; DNA CLEAVAGE; ENZYME ACTIVITY; HYDROLYSIS; MUTANT; NONHUMAN; PRIORITY JOURNAL; VACCINIA VIRUS;

BINDING SITES; CYSTEINE; DNA; DNA TOPOISOMERASES, TYPE I; DNA, SUPERHELICAL; ENDONUCLEASES; GLUTAMIC ACID; HISTIDINE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MUTAGENESIS; STRUCTURE-ACTIVITY RELATIONSHIP; TYROSINE; VACCINIA VIRUS;

VACCINIA; VACCINIA VIRUS;

EID: 0032518166     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/26.2.490     Document Type: Article

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