Change of a catalytic reaction carried out by a DNA replication protein

Science

Volumn 274, Issue 5288, 1996, Pages 777-780

  Noirot Gros, Marie Françoise ^a,b   Ehrlich, Stanislav D ^a  

^a CEMAGREF   (France)

^b HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HELICASE; PHOSPHOTYROSINE;

ARTICLE; CATALYSIS; DNA CLEAVAGE; DNA RECOMBINATION; DNA REPLICATION; DNA STRAND; DNA TRANSFER; ESTERIFICATION; PRIORITY JOURNAL;

BACTERIOPHAGE PHI X 174; BINDING SITES; DNA HELICASES; DNA REPLICATION; DNA, BACTERIAL; DNA, SINGLE-STRANDED; DNA, VIRAL; DNA-BINDING PROTEINS; ESTERIFICATION; EVOLUTION, MOLECULAR; GLUTAMIC ACID; HYDROLYSIS; MUTATION; PLASMIDS; PROTEINS; TRANS-ACTIVATORS; TYROSINE; VIRAL PROTEINS;

EID: 0029908732     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5288.777     Document Type: Article

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10. 210EMAKY (9), respectively. However, valine instead of methionine is present in several Rep proteins of the pC194 family (7), and the two residues are therefore functionally equivalent.
11. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, IIe; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
12. M.-F. Noirot-Gros and S. D. Ehrlich, data not shown.
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15. Quantification was done with a Phosphorimager SI (Molecular Dynamics).
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17. We thank M. A. Petit tor stimulating discussions during the course of this work and P. Noirot and R. Benneth for critical reading of this manuscript. We particularly thank R. Hanai and J. C. Wang for useful comments and help in the preparation of this manuscript.