Identification of catalytic bases in the active site of Escherichia coli methylglyoxal synthase: Cloning, expression, and functional characterization of conserved aspartic acid residues

Biochemistry

Volumn 37, Issue 28, 1998, Pages 10074-10086

  Saadat, Dana ^a   Harrison, David H T ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; BACTERIAL ENZYME; DIHYDROXYACETONE PHOSPHATE; METHYLGLYCOXAL SYNTHASE; POLYPEPTIDE; TRIOSEPHOSPHATE ISOMERASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; ENZYME ASSAY; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN QUATERNARY STRUCTURE; SEQUENCE ANALYSIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ASPARTIC ACID; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; BINDING, COMPETITIVE; CARBON-OXYGEN LYASES; CATALYSIS; CIRCULAR DICHROISM; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, BACTERIAL; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GLYCOLATES; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHATES; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS;

EID: 0032516436     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi980409p     Document Type: Article

References (40)

1. Murata, K., Fukuda, Y., Watanabe, K., Saikusa, T., Shimosaka, M., and Kimura, A. (1985) Biochem. Biophys. Res. Commun. 131, 190-198.
2. Ray, S., and Ray, M. (1981) J. Biol. Chem. 256, 6230-6233.
3. Thornalley, P. J. (1990) Biochem. J. 269, 1-11.
4. Cooper, R. A., and Anderson, A. (1970) FEBS Lett. 11, 273-276.
5. Cooper, R. A. (1984) Annu. Rev. Microbiol. 44, 812-826.
6. Fareleira, P., Legall, J., Xavier, A. V., and Santos, H. (1997) J. Bacteriol. 179, 3972-3980.
7. Rahman, A., Shahabuddin, and Hadi, S. M. (1990) J. Biochem. Toxicol. 5, 161-166.
8. Papoulis, A., al-Abed, Y., and Bucala, R. (1995) Biochemistry 34, 648-655.
9. Baskaran, S., and Balasubramanian, K. A. (1990) Biochem. Int. 21, 165-174.
10. Együd, L. G., and Szent-Györgyi, A. (1966) Proc. Natl. Acad. U.S.A. 56, 203-207.
11. Brownlee, M., and Cerami, A. (1981) Annu. Rev. Biochem. 50, 385-432.
12. Hopper, D. J., and Cooper, R. A. (1972) Biochem. J. 128, 321-329.
13. Cooper, R. A. (1974) Eur. J. Biochem. 44, 81-86.
14. Tsai, P., and Gracy, R. W. (1976) J. Biol. Chem. 251, 365-367.
15. Iyengar, R., and Rose, I. A. (1983) J. Am. Chem. Soc. 105, 3301-3303.
16. Murthy, N. S., Bakeris, T., Kavarana, M. J., Hamilton, D. S., Lan, Y., and Creighton, D. J. (1994) J. Med. Chem. 37, 2161-2166.
17. Summers, M. C., and Rose, I. A. (1977) J. Am. Chem. Soc. 99, 4475-4478.
18. Alber, T. C., Davenport, R. C., Jr., Giammona, D. A., Lolis, E., Petsko, G. A., and Ringe, D. (1987) Cold Spring Harbor Symp. Quant. Biol. 52, 603-613.
19. Joseph, D., Petsko, G. A., and Karplus, M. (1990) Science 249, 1425-1428.
20. Pompliano, D. L., Peyman, A., and Knowles, J. R. (1990) Biochemistry 29, 3186-3194.
21. Percy, D. S., and Harrison, D. H. T. (1996) FASEB J. 10, 1367.
22. Tötemeyer, S., Booth, N. A., Nichols, W. W., Dunbar, B., and Booth, I. R. (1998) Mol. Microbiol. 27, 553-562.
23. Straus, D., Raines, R., Kawashima, E., Knowles, J. R., and Gilbert, W. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 2272-2276.
24. Joseph-McCarthy, D., Rost, L. E., Komives, E. A., and Petsko, G. A. (1994) Biochemistry 33, 2824-2829.
25. Straub, A., and Effenberger, F. (1987) Tetrahedron Lett. 28, 1641-1644.
26. Bradford, M. (1976) Anal. Biochem. 72, 248-254.
27. Ornstein, L., and Davis, B. J. (1964) Ann. N. Y. Acad. Sci. 121, 321.
28. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K., and Pease, L. R. (1989) Gene 77, 51-59.
29. Leatherbarrow, R. J. (1994) GraFit: Data Analysis and Graphics Program.
30. Segel, I. H. (1975) Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems, John Wiley and Sons, New York.
31. Hobohm, U., Houthaeve, T., and Sander, C. (1994) Anal. Biochem. 222, 202.
32. Pearson, W. R., and Lipman, D. J. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 2444-2448.
33. Wood, E. R., and Matson, S. W. (1989) J. Biol. Chem. 264, 8297-8303.
34. Blattner, F. R., Plunkett, G., III, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., and Shao, Y. (1997) Science 277, 1453-1474.
35. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990) J. Mol. Biol. 215, 403-410.
36. Hopper, D. J., and Cooper, R. A. (1971) FEBS Lett. 13, 213-216.
37. Wolfenden, R. (1970) Biochemistry 9, 3404-3407.
38. Lolis, E., and Petsko, G. A. (1990) Biochemistry 29, 6619-6625.
39. Genetics Computer Group (GCG). (1982) Wisconsin Package.
40. Joseph-McCarthy, D., Petsko, G. A., and Karplus, M. (1995) Protein Eng. 8, 1103-1115.