The ubiquitin-related protein RUB1 and auxin response in Arabidopsis

Science

Volumn 280, Issue 5370, 1998, Pages 1760-1763

  Del Pozo, J C ^a   Timpte, C ^a,c   Tan, S ^b   Callis, J ^b   Estelle, M ^a  

^a INDIANA UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF NEW ORLEANS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AUXIN; CYSTEINE; DITHIOTHREITOL; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PROTEIN; THIOESTER; UBIQUITIN;

ARABIDOPSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; GENE; GENE MAPPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE TAGGED SITE;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CELL NUCLEUS; DITHIOTHREITOL; GENE EXPRESSION; GROWTH SUBSTANCES; INDOLEACETIC ACIDS; LIGASES; MOLECULAR SEQUENCE DATA; MUTATION; PLANT PROTEINS; RECOMBINANT FUSION PROTEINS; UBIQUITIN-ACTIVATING ENZYMES; UBIQUITIN-PROTEIN LIGASES; UBIQUITINS;

EID: 0032511111     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5370.1760     Document Type: Article

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21. 2 and ∼200 cps of labeled protein. This mixture was incubated for 30 min at 23°C, and half of the reaction was stopped for 15 min with 4% SDS/10% glycerol and the other half with SDS/glycerol and 100 mM DTT.
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23. 2, and ∼400 cps of labeled RUB1. The reaction was performed at room temperature for 45 min and stopped as described (13).
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29. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
30. We thank J. Turner (J.C.d.P., C.T., M.E.), B. McArdle, and J. Martin (S.T., J.C) for technical assistance. Supported by NSF grant 93-06759 (J.C.) and NIH grant GM43644 (M.E.). J.C.d.P. was supported by a long-term fellowship from the Spanish Government.