메뉴 건너뛰기




Volumn 281, Issue 5383, 1998, Pages 1683-1686

Biological action of leptin as an angiogenic factor

Author keywords

[No Author keywords available]

Indexed keywords

ANGIOGENIC FACTOR; LEPTIN; LEPTIN RECEPTOR;

EID: 0032508682     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5383.1683     Document Type: Article
Times cited : (1298)

References (39)
  • 1
    • 0030902584 scopus 로고    scopus 로고
    • J. S. Flier, Proc. Natl. Acad. Sci. U.S.A. 94, 4242 (1997); N. Levin, C. Nelson, A. Gurney, R. Vandlen, F. de Sauvage, ibid. 93, 1726 (1996); M. A. Pelleymounter et al., Science 269, 540 (1995); J. L. Halaas et al., ibid., p. 543; L. A. Campfield, F. J. Smith, Y. Guisez, R. Devos, P. Burn, ibid., p. 546.
    • (1997) Proc. Natl. Acad. Sci. U.S.A. , vol.94 , pp. 4242
    • Flier, J.S.1
  • 2
    • 0029939065 scopus 로고    scopus 로고
    • J. S. Flier, Proc. Natl. Acad. Sci. U.S.A. 94, 4242 (1997); N. Levin, C. Nelson, A. Gurney, R. Vandlen, F. de Sauvage, ibid. 93, 1726 (1996); M. A. Pelleymounter et al., Science 269, 540 (1995); J. L. Halaas et al., ibid., p. 543; L. A. Campfield, F. J. Smith, Y. Guisez, R. Devos, P. Burn, ibid., p. 546.
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 1726
    • Levin, N.1    Nelson, C.2    Gurney, A.3    Vandlen, R.4    De Sauvage, F.5
  • 3
    • 0029066265 scopus 로고
    • J. S. Flier, Proc. Natl. Acad. Sci. U.S.A. 94, 4242 (1997); N. Levin, C. Nelson, A. Gurney, R. Vandlen, F. de Sauvage, ibid. 93, 1726 (1996); M. A. Pelleymounter et al., Science 269, 540 (1995); J. L. Halaas et al., ibid., p. 543; L. A. Campfield, F. J. Smith, Y. Guisez, R. Devos, P. Burn, ibid., p. 546.
    • (1995) Science , vol.269 , pp. 540
    • Pelleymounter, M.A.1
  • 4
    • 0030902584 scopus 로고    scopus 로고
    • J. S. Flier, Proc. Natl. Acad. Sci. U.S.A. 94, 4242 (1997); N. Levin, C. Nelson, A. Gurney, R. Vandlen, F. de Sauvage, ibid. 93, 1726 (1996); M. A. Pelleymounter et al., Science 269, 540 (1995); J. L. Halaas et al., ibid., p. 543; L. A. Campfield, F. J. Smith, Y. Guisez, R. Devos, P. Burn, ibid., p. 546.
    • Science , pp. 543
    • Halaas, J.L.1
  • 5
    • 0029048408 scopus 로고    scopus 로고
    • J. S. Flier, Proc. Natl. Acad. Sci. U.S.A. 94, 4242 (1997); N. Levin, C. Nelson, A. Gurney, R. Vandlen, F. de Sauvage, ibid. 93, 1726 (1996); M. A. Pelleymounter et al., Science 269, 540 (1995); J. L. Halaas et al., ibid., p. 543; L. A. Campfield, F. J. Smith, Y. Guisez, R. Devos, P. Burn, ibid., p. 546.
    • Science , pp. 546
    • Campfield, L.A.1    Smith, F.J.2    Guisez, Y.3    Devos, R.4    Burn, P.5
  • 6
    • 0030898516 scopus 로고    scopus 로고
    • M. Shimabukuro et al., Proc. Natl. Acad. Sci. U.S.A. 94, 4637 (1997); T. Gainsford et al., ibid. 93, 14564 (1996); T. J. Kieffer, R. S. Heller, C. A. Leech, G. G. Holz, J. F. Habener, Diabetes 46, 1087 (1997); R. J. Zachow and D. A. Magoffin, Endocrinology 138, 847 (1997); J. A. Tuominen, P. Ebeling, M. L. Heiman, T. Stephens, V. A. Koivisto, Acta Physiol. Scand. 160, 83 (1997).
    • (1997) Proc. Natl. Acad. Sci. U.S.A. , vol.94 , pp. 4637
    • Shimabukuro, M.1
  • 7
    • 0029952911 scopus 로고    scopus 로고
    • M. Shimabukuro et al., Proc. Natl. Acad. Sci. U.S.A. 94, 4637 (1997); T. Gainsford et al., ibid. 93, 14564 (1996); T. J. Kieffer, R. S. Heller, C. A. Leech, G. G. Holz, J. F. Habener, Diabetes 46, 1087 (1997); R. J. Zachow and D. A. Magoffin, Endocrinology 138, 847 (1997); J. A. Tuominen, P. Ebeling, M. L. Heiman, T. Stephens, V. A. Koivisto, Acta Physiol. Scand. 160, 83 (1997).
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 14564
    • Gainsford, T.1
  • 8
    • 0030948734 scopus 로고    scopus 로고
    • M. Shimabukuro et al., Proc. Natl. Acad. Sci. U.S.A. 94, 4637 (1997); T. Gainsford et al., ibid. 93, 14564 (1996); T. J. Kieffer, R. S. Heller, C. A. Leech, G. G. Holz, J. F. Habener, Diabetes 46, 1087 (1997); R. J. Zachow and D. A. Magoffin, Endocrinology 138, 847 (1997); J. A. Tuominen, P. Ebeling, M. L. Heiman, T. Stephens, V. A. Koivisto, Acta Physiol. Scand. 160, 83 (1997).
    • (1997) Diabetes , vol.46 , pp. 1087
    • Kieffer, T.J.1    Heller, R.S.2    Leech, C.A.3    Holz, G.G.4    Habener, J.F.5
  • 9
    • 0031046147 scopus 로고    scopus 로고
    • M. Shimabukuro et al., Proc. Natl. Acad. Sci. U.S.A. 94, 4637 (1997); T. Gainsford et al., ibid. 93, 14564 (1996); T. J. Kieffer, R. S. Heller, C. A. Leech, G. G. Holz, J. F. Habener, Diabetes 46, 1087 (1997); R. J. Zachow and D. A. Magoffin, Endocrinology 138, 847 (1997); J. A. Tuominen, P. Ebeling, M. L. Heiman, T. Stephens, V. A. Koivisto, Acta Physiol. Scand. 160, 83 (1997).
    • (1997) Endocrinology , vol.138 , pp. 847
    • Zachow, R.J.1    Magoffin, D.A.2
  • 10
    • 0030906928 scopus 로고    scopus 로고
    • M. Shimabukuro et al., Proc. Natl. Acad. Sci. U.S.A. 94, 4637 (1997); T. Gainsford et al., ibid. 93, 14564 (1996); T. J. Kieffer, R. S. Heller, C. A. Leech, G. G. Holz, J. F. Habener, Diabetes 46, 1087 (1997); R. J. Zachow and D. A. Magoffin, Endocrinology 138, 847 (1997); J. A. Tuominen, P. Ebeling, M. L. Heiman, T. Stephens, V. A. Koivisto, Acta Physiol. Scand. 160, 83 (1997).
    • (1997) Acta Physiol. Scand. , vol.160 , pp. 83
    • Tuominen, J.A.1    Ebeling, P.2    Heiman, M.L.3    Stephens, T.4    Koivisto, V.A.5
  • 11
    • 0029895233 scopus 로고    scopus 로고
    • J. G. Mercer et al., FEBS Lett. 387, 113 (1996); C. Vaisse et al., Nature Genet. 14, 95 (1996).
    • (1996) FEBS Lett. , vol.387 , pp. 113
    • Mercer, J.G.1
  • 12
    • 0029739826 scopus 로고    scopus 로고
    • J. G. Mercer et al., FEBS Lett. 387, 113 (1996); C. Vaisse et al., Nature Genet. 14, 95 (1996).
    • (1996) Nature Genet. , vol.14 , pp. 95
    • Vaisse, C.1
  • 13
    • 0030039272 scopus 로고    scopus 로고
    • G. H. Lee et al., Nature 379, 632 (1996).
    • (1996) Nature , vol.379 , pp. 632
    • Lee, G.H.1
  • 15
    • 0029776030 scopus 로고    scopus 로고
    • H. Baumann et al., Proc. Natl. Acad. Sci. U.S.A. 93, 8374 (1996); N. Ghilardi et al., ibid., p. 6231; C. Bjøbæk et al., J. Biol. Chem. 272, 32686 (1997).
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 8374
    • Baumann, H.1
  • 16
    • 0029776030 scopus 로고    scopus 로고
    • H. Baumann et al., Proc. Natl. Acad. Sci. U.S.A. 93, 8374 (1996); N. Ghilardi et al., ibid., p. 6231; C. Bjøbæk et al., J. Biol. Chem. 272, 32686 (1997).
    • Proc. Natl. Acad. Sci. U.S.A. , pp. 6231
    • Ghilardi, N.1
  • 17
    • 0031436148 scopus 로고    scopus 로고
    • H. Baumann et al., Proc. Natl. Acad. Sci. U.S.A. 93, 8374 (1996); N. Ghilardi et al., ibid., p. 6231; C. Bjøbæk et al., J. Biol. Chem. 272, 32686 (1997).
    • (1997) J. Biol. Chem. , vol.272 , pp. 32686
    • Bjøbæk, C.1
  • 20
    • 3543094188 scopus 로고    scopus 로고
    • note
    • ext antibodies to extracellular epitopes of the leptin receptor.
  • 22
    • 3543116391 scopus 로고    scopus 로고
    • note
    • int, Ulex europaeus agglutinin I, or normal rabbit serum. After incubation with primary antibodies, tissue sections were developed with secondary horseradish peroxidase-conjugated goat antibody to rabbit IgG with a Vectastain Elite ABC kit (Vector Labs). The slides were counterstained with hematoxylin.
  • 23
    • 3543142129 scopus 로고    scopus 로고
    • note
    • int (9) or with RC20 recombinant anti-phosphotyrosine (Transduction Laboratories, Lexington, KY).
  • 24
    • 3543073187 scopus 로고    scopus 로고
    • note
    • 705) rabbit polyclonal antibody, was used for detecting the presence of Stat3 and phospho-Stat3 in the cell lysates.
  • 25
    • 3543066072 scopus 로고    scopus 로고
    • note
    • 2, 20 mM KCl, 0.2 mM EDTA, 0.5 mM DTT, 0.2 mM phenytmethylsulfonyl fluoride, and a mixture of protease inhibitors]. After a 15-min incubation on ice, cells were homogenized, and nuclei were collected by centrifugation. The pellet was resuspended in low-salt buffer adjusted to 0.5 M KCl, extracted for 30 min at 4°C, and centrifuged at 25,000g for 30 min. Supernates were collected, dialyzed against buffer made 0.1 M with KCl, and divided into small portions kept at -80°C until used.
  • 26
    • 3543114088 scopus 로고    scopus 로고
    • note
    • 32P-labeled probe for 20 min at room temperature in binding buffer [20 mM tris-HCl (pH 7.9), 50 mM NaCl, 1 mM EDTA, 10% glycerol, 0.1% NP-40, 1 mM DTT, bovine serum albumin (50 μg/ ml), and polydeoxyinosine/polydeoxycytosine (2 μg/ ml)]. Electrophoresis sample buffer was added to each sample before separation in a nondenaturing gel (89 mM tris, 89 mM boric acid, 10 mM EDTA, and 4.95% acrylamide). Gels were dried and exposed to autoradiography.
  • 28
  • 32
    • 3543070821 scopus 로고    scopus 로고
    • note
    • 2 incubator at 37°C for 2 to 5 min, allowing them to solidify. Growth medium supplemented with endothelial cell growth supplement with or without human recombinant leptin was then added to each dish. Leptin was replenished every 24 hours. HUVECs were allowed to form tubelike structures for 2 days in culture and were then frozen and sectioned to a thickness of 40 μm. For immunofluorescence analysis, the sections were fixed with acetone (-20°C) and stained with tetramethylrhodamine isothiocyanate-labeled Ulex europaeus agglutinin I (0.5 μg/ml) and 4′,6′-diamidino-2-phenylindole (DAPI) (0.0001%).
  • 34
    • 0030871847 scopus 로고    scopus 로고
    • K. Koyama et al., Diabetes 48, 1276 (1997).
    • (1997) Diabetes , vol.48 , pp. 1276
    • Koyama, K.1
  • 37
    • 3543057888 scopus 로고    scopus 로고
    • note
    • Abbreviations for the amino acid residues are as follows: C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
  • 39
    • 3543055514 scopus 로고    scopus 로고
    • note
    • We thank T. Buckholz and J. Kupcho for the synthetic peptides and conjugates, L. Friedman for the anti-leptin receptor, L. Benson and G. Davis for HUVEC cultures, D. O'Connor for help in migration studies, and J. Pober for laboratory resources.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.