An orthogonal solid phase support for the synthesis of oligonucleotides containing 3'-phosphates and its application in the preparation of photolabile hybridization probes

Tetrahedron Letters

Volumn 39, Issue 24, 1998, Pages 4155-4158

  McMinn, Dustin L ^a   Hirsch, Robert ^a   Greenberg, Marc M ^a  

^a Immunology and Pathology   (United States)

Author keywords

Nucleic acids; Photochemistry; Solid phase synthesis

Indexed keywords

AMMONIA; BIOPOLYMER; OLIGONUCLEOTIDE;

ARTICLE; BASE MISPAIRING; DRUG SYNTHESIS; HYBRIDIZATION; OLIGONUCLEOTIDE PROBE; PHOTOCHEMISTRY;

EID: 0032508072     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(98)00773-4     Document Type: Article

References (20)

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19. 12. Unlike LCAA-CPG, Tentagel® does not contain any base labile components. Also, Tentagel® has superior swelling properties relative to LCAA-CPG, which we anticipated would improve the hybridization efficiency of oligonucleotides prepared on the support.
20. 32P-labeled oligonucleotide in 100 μL phosphate buffer (10 mM, pH 7.5) containing NaCl (0.1 M) to 90 °C for 10 min, the mixture was allowed to cool in the heat block to 45 °C. The supernatants were removed via pipet, and the resin washed with phosphate buffer (3 × 50 μL). Photolyses were carried out in eppendorf tubes containing phosphate buffer using a transilluminator as described in reference 5. The supernatant was removed and analyzed by Cerenkov counting and/or nondenaturing polyacrylamide gel electrophoresis.