Pre-steady-state and steady-state kinetic studies on transcription initiation catalyzed by T7 RNA polymerase and its active-site mutants K631R and Y639F

Biochemistry

Volumn 37, Issue 45, 1998, Pages 15958-15964

  Woody, A Young Moon ^a   Osumi Davis, Patricia A ^a   Hiremath, Meenaxi M ^b   Woody, Robert W ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARTICLE; CHEMICAL BOND; COMPLEX FORMATION; DISSOCIATION CONSTANT; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; EQUILIBRIUM CONSTANT; GEL ELECTROPHORESIS; MUTANT; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN DNA BINDING; STEADY STATE; TRANSCRIPTION INITIATION;

AMINO ACID SUBSTITUTION; ARGININE; BINDING SITES; CATALYSIS; DNA-DIRECTED RNA POLYMERASES; ENZYME STABILITY; KINETICS; LYSINE; MACROMOLECULAR SUBSTANCES; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; TEMPLATES, GENETIC; TRANSCRIPTION, GENETIC; TYROSINE; VIRAL PROTEINS;

EID: 0032506259     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9805801     Document Type: Article

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