Efficient gene trap screening for novel developmental genes using IRESβgeo vector and in vitro preselection

Experimental Cell Research

Volumn 244, Issue 1, 1998, Pages 125-136

  Bonaldo, Paolo ^a,b   Chowdhury, Kamal ^a   Stoykova, Anastasia ^a   Torres, Miguel ^a,c   Gruss, Peter ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF PADOVA   (Italy)

^c UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

Embryonic stem cells; Gene trap; Insertional mutagenesis; IRES geo vector; Mouse development

Indexed keywords

ANIMAL CELL; ARTICLE; DEVELOPMENTAL GENETICS; EXPRESSION VECTOR; GENE ACTIVATION; GENE CONTROL; GENE EXPRESSION; GENE INSERTION; GENE MUTATION; GENE REPRESSION; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; MAMMALIA;

EID: 0032505679     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1998.4208     Document Type: Article

References (28)

1. Gossler, A., Joyner, A. L., Rossant, J., and Skarnes, W. C. (1989). Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244, 463-465.
2. Friedrich, G., and Soriano, P. (1991). Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. Genes Dev. 5, 1513-1523.
3. Skarnes, W. C., Auerbach, B. A., and Joyner, A. L. (1992). A gene trap approach in mouse embryonic stem cells: The lacZ reporter gene is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev. 6, 903-918.
4. Frohman, M. A., Dush, M. K., and Martin, G. R. (1988). Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002.
5. Chowdhury, K., Bonaldo, P., Torres, M., Stoykova, A., and Grass, P. (1997). Evidence for the stochastic integration of gene trap vectors into the mouse germ line. Nucleic Acids Res. 25, 1531-1536.
6. Chen, Z., Friedrich, G. A., and Soriano, P. (1994). Transcriptional enhancer factor I disruption by a retroviral gene trap leads to heart defects and embryonic lethality in mice. Genes Dev. 8, 2293-2301.
7. DeGregori, J., Russ, A., von Melchner, H., Rayburn, H., Priyaranjan, P., Jenkins, N. A., Copeland, N., and Ruley, H. E. (1994). A murine homolog of the yeast RNA1 gene is required for postimplantation development. Genes Dev. 8, 265-276.
8. Takeuchi, T., Yamazaki, Y., Katoh-Fukui, Y., Tsuchya, R., Kondo, S., Motoyama, J., and Higashinakagawa, T. (1995). Gene trap capture of a novel mouse gene, jumonji, required for neural tube formation. Genes Dev. 9, 1211-1222.
9. Serafini, T., Colamarino, S. A., Leonardo, E. D., Wang, H., Beddington, R., Skarnes, W. C., and Tessier-Lavigne, M. (1996). Netrin-1 is required for commissural axon guidance in the developing vertebrate nervous system. Cell 87, 1001-1014.
10. Torres, M., Stoykova, A., Huber, O., Chowdhury, K., Bonaldo, P., Mansouri, A., Butz, S., Kemler, R., and Gruss, P. (1997). An α-E-catenin gene trap mutation defines its function in preimplantation development. Proc. Natl. Acad. Sci. USA 94, 901-906.
11. Yeo, T. T., Yang, T., Massa, S. M., Zhang, J. S., Honkaniemi, J., Butcher, L. L., and Longo, F. M. (1997). Deficient LAR expression decreases basal forebrain cholinergic neuronal size and hippocampal cholinergic innervation. J. Neurosci. Res. 47, 348-360.
12. Skarnes, W. C., Moss, J., Hurtley, S., and Beddington, R. (1995). Capturing genes encoding membrane and secreted proteins important for mouse development. Proc. Natl. Acad. Sci. USA 92, 6592-6596.
13. Forrester, L. M., Nagy, A., Sam, M., Watt, A., Stevenson, L., Bernstein, A., Joyner, A. L., and Wurst, W. (1996). An induction gene trap screen in embryonic stem cells: Identification of genes that respond to retinoic acid in vitro. Proc. Natl. Acad. Sci. USA 93, 1677-1688.
14. Baker, R. K., Haendel, M. A., Swanson, B. J., Shambaugh, J. C., Micales, B. K., and Lyons, G. E. (1997). In vitro preselection of gene-trapped embryonic stem cell clones for characterizing novel developmentally, regulated genes in the mouse. Dev. Biol. 185, 201-214.
15. Mountford, P. S., and Smith, A. G. (1995). Internal ribosome entry sites and dicistronic RNAs in mammalian transgenesis. Trends Genet. 11, 179-184.
16. Kim, D. G., Kang, H. M., Jang, S. K., and Shin, H.-S. (1992). Construction of a bifunctional mRNA in the mouse by using the internal ribosome entry site of the encephalomyocarditis virus. Mol. Cell Biol. 12, 3636-3643.
17. Nagy, A., Rossant, J., Nagy, R., Abramow-Newerly, W., and Roder, J. C. (1993). Derivation of completely cell culture-derived mice from early passage embryonic stem cells. Proc. Natl. Acad. Sci. USA 90, 8424-8428.
18. Gossler, A., and Zachgo, J. (1993). Gene and enhancer trap screens in ES cell chimeras. In "Gene Targeting: A Practical Approach" (A. L. Joyner, Ed.), pp. 181-225, IRL Press, Oxford.
19. Nagy, A., and Rossant, J. (1993). Production of completely ES cell-derived fetuses. In "Gene Targeting: A Practical Approach" (A. L. Joyner, Ed.), pp. 147-178, IRL Press, Oxford.
20. Stoykova, A., Chowdhury, K., Bonaldo, P., Torres, M., and Grass, P. (1998). Gene trap expression and mutational analysis for genes involved in the development of the mammalian nervous system. Dev. Dynamics 212, 198-213.
21. Hemmati-Brivanlou, A., Kelly, O. G., and Melton, D. A. (1994). Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activity. Cell 77, 283-295.
22. Mountford, P., Zevnik, B., Düwel, A., Nichols, J., Li, M., Dani, C., Robertson, M., Chambers, I., and Smith, A. (1994). Dicistronic targeting constructs: Reporters and modifiers of mammalian gene expression. Proc. Natl. Acad. Sci. USA 91, 4303-4307.
23. Würst, W., Rossant, J., Prideux, V., Kownacka, M., Joyner, A., Hill, D. P., Guillermot, F., Gasca, S., Cado, D., Auerbach, A., and Ang, S.-L. (1995). A large-scale gene trap screen for insertional mutations in developmentally regulated genes in mice. Genetics 139, 889-899.
24. Weiss, M. J., and Orkin, S. H. (1996). In vitro differentiation of murine embryonic stem cells: New approaches to old problems. J. Clin. Invest. 97, 591-595.
25. Bain, G., Kitchens, D., Yao, M., Huettner, J. E., and Gottlieb, D. I. (1995). Embryonic stem cells express neuronal properties in vitro. Dev. Biol. 168, 342-357.
26. Jelsma, T. N., and Aguayo, A. J. (1994). Trophic factors. Curr. Opin. Neurobiol. 4, 717-725.
27. Wobus, A. M., Grosse, R., and Schöneich, J. (1988). Specific effects of nerve growth factor on the differentiation pattern of mouse embryonic stem cells in vitro. Biomed. Biochim. Acta 47, 965-973.
28. Zernicka-Goetz, M., Pines, J., McLean-Hunter, S., Dixon, J. P., Siemering, K. R., Haseloff, J., and Evans, M. J. (1997). Following cell fate in the living mouse embryo. Development 124, 1133-1137.