Conservation of substrate recognition mechanisms by tRNA splicing endonucleases

Science

Volumn 280, Issue 5361, 1998, Pages 284-286

  Fabbri, Stefania ^a   Fruscoloni, Paolo ^b   Bufardeci, Emanuela ^a   Di Nicola Negri, Elisa ^b   Baldi, Maria I ^b   Gandini Attardi, Domenica ^b   Mattoccia, Emilio ^b   Tocchini Valentini, Glauco P ^b,c  

^a Istituto Guido Donegani SpA   (Italy)

^b CNR   (Italy)

^c UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; ENDONUCLEASE; FUNGAL PROTEIN; TRANSFER RNA;

ARCHAEBACTERIUM; ARTICLE; ENZYME SPECIFICITY; ENZYME SUBSTRATE; INTRON; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; RNA BINDING; RNA SPLICING; SACCHAROMYCES CEREVISIAE; XENOPUS LAEVIS;

ANIMALS; ANTICODON; BASE COMPOSITION; BASE SEQUENCE; ENDORIBONUCLEASES; INTRONS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RNA PRECURSORS; RNA SPLICING; RNA, ARCHAEAL; RNA, TRANSFER, PHE; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY; XENOPUS;

ARCHAEA; BACTERIA (MICROORGANISMS); EUKARYOTA; SACCHAROMYCES CEREVISIAE; XENOPUS LAEVIS;

EID: 0032502990     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5361.284     Document Type: Article

References (13)

1. M. Belfort and A. Weiner, Cell 89, 1003 (1997).
2. V. M. Reyes and J. Abelson, ibid. 55, 719 (1988).
3. E. Mattoccia, M. I. Baldi, D. Gandini Attardi, S. Ciafrè, G. P. Tocchini-Valentini, ibid., p. 731.
4. L. D. Thompson and C. J. Daniels, J. Biol. Chem. 265, 18104 (1990).
5. M. I. Baldi, E. Mattoccia, E. Bufardeci, S. Fabbri, G. P. Tocchini-Valentini, Science 255, 1404 (1992).
6. S. Fabbri et al., data not shown.
7. E. Di Nicola Negri et al., Cell 89, 859 (1997).
8. H. Li, C. R. Trotta, J. Abelson, Science 280, 279 (1998).
9. C. R. Trotta et al., Cell 89, 849 (1997).
10. D. Gandini Attardi, M. I. Baldi, E. Mattoccia, G. P. Tocchini-Valentini, Methods Enzymol. 181, 510 (1989).
11. L. D. Thompson and C. J. Daniels, J. Biol. Chem. 263, 1751 (1988).
12. ∇ were synthesized as described (3, 5, 7). Templates for the synthesis of the Archaeuka precursors were constructed by polymerase chain reaction (PCR). The PCR templates were the full-length pre-tRNAs. One primer contained the T7 promoter and part of the 5′ exon. The other was composed of the desired sequence of the 3′ exon. Conditions for PCR, transcription by T7 RNA polymerase, and endonuclease assays were as described (3, 5, 7). Xenopus laevis endonuclease was purified as in (10), Saccharomices cerevisiae endonuclease as in (9), and S. sulfataricus endonuclease as in (11).
13. We thank J. Dahlberg for help with the manuscript; J. Abelson for helpful comments, for communicating results before publication, and for yeast endonuclease; R. H. Haselkorn for critical reading of the manuscript; M. Rossi for S. sulfataricus cells; G. Di Franco for technical assistance; and A. Sebastiano for typesetting help with the manuscript. Supported in part by Progetto Finalizzato (CNR) Ingegneria Genetica, Progetto Finalizzato (CNR) Biotecnologie, Human Frontiers Science Program Organization (HFSPO), and Fondo Cinquepercento: Biotecnologie.