1H-NMR on line monitoring of water activity during lipase catalysed esterification

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1387, Issue 1-2, 1998, Pages 129-135

  Decagny, Benoît ^a   Roblot, Corinne ^a   Ergan, Françoise ^b   Sarazin, Catherine ^a   Barbotin, Jean Noël ^a   Séguin, Jean Paul ^a  

^a UNIVERSITÉ DE PICARDIE JULES VERNE   (France)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

Esterification; Lipase; NMR; Water activity

Indexed keywords

TRIACYLGLYCEROL LIPASE; WATER; ALCOHOL DERIVATIVE; BUTANOL; CARBOXYLIC ACID; ESTER; FUNGAL PROTEIN; HYDROGEN; HYDROXIDE; HYDROXIDE ION; OCTANOIC ACID; OCTANOIC ACID DERIVATIVE;

ARTICLE; CALIBRATION; CATALYSIS; ESTERIFICATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; TIME; WATER CONTENT; CHEMISTRY; ENZYMOLOGY; KINETICS; METABOLISM; MUCORALES;

1-BUTANOL; ALCOHOLS; CARBOXYLIC ACIDS; ESTERIFICATION; ESTERS; FUNGAL PROTEINS; HYDROGEN; HYDROXIDES; KINETICS; LIPASE; MAGNETIC RESONANCE SPECTROSCOPY; MUCORALES; OCTANOIC ACIDS; WATER;

EID: 0032497167     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(98)00114-9     Document Type: Article

References (13)

1. Tuena de Gomez-Puyou M., Gomez-Puyou A. Enzymes in low water systems. Crit. Rev. Biochem. Mol. Biol. 33:1998;53-89.
2. Casa R.M., Sanchez-Montero J.M., Sinisterra J.V. Water adsorption isotherm as a tool to predict the preequilibrium water amount in preparative esterification. Biotechnol. Lett. 18:1996;13-18.
3. Bell G., Halling P.J., Moore B.D., Partridge J., Rees D.G. Biocatalyst behaviour in low-water systems. Trends Biotechnol. 13:1995;468-473.
4. Valivety R.H., Halling P.J., Peilow A.D., Macrae A.D. Lipases from different sources vary widely in dependence of catalytic activity on water activity. Biochim. Biophys. Acta. 1122:1992;143-146.
5. Halling P.J. Thermodynamic predictions for biocatalysis in non conventional media: Theory, tests, and recommendations for experimental design and analysis. Enzyme Microb. Technol. 16:1994;178-206.
6. Throller J. Methods to measure water activity. J. Food Prot. 46:1983;129-134.
7. Blyth J., Millington R.B., Mayes A.G., Frears E.R., Lowe C.R. Holographic sensor for water in solvents. Anal. Chem. 68:1996;1089-1094.
8. Wehtje E., Kaur J., Adlercreutz P., Chand S., Mattiason B. Water activity control in enzymatic esterification processes. Enzyme Microb. Technol. 21:1997;502-510.
9. Jenta T.R.J., Batts G., Rees G.D., Robinson B.H. Kinetic studies of Chromobacterium viscosum lipase in AOT water in oil macroemulsions and gelatin microemulsion-based organogels. Biotechnol. Bioeng. 54:1997;416-427.
10. Sarazin C., Ergan F., Séguin J.P., Goethals G., Legoy M.D., Barbotin J.N. NMR on-line monitoring of esterification catalyzed by cutinase. Biotechnol. Bioeng. 51:1996;636-644.
11. Parker M.C., Moore B.D., Blacker A.J. Measuring enzyme hydration in nonpolar organic solvents using NMR. Biotechnol. Bioeng. 46:1995;452-458.
12. 1H nuclear magnetic resonance spectroscopy: application to the study of water exchange in biphasic media. Anal. Biochem. 234:1996;142-148.
13. Bell G., Janssen A.E.M., Halling P.J. Water activity fails to predict critical hydratation level for enzyme activity in polar organic solvents: interconversion of water concentrations and activities. Enzyme Microb. Technol. 20:1997;471-477.