Water activity fails to predict critical hydration level for enzyme activity in polar organic solvents: Interconversion of water concentrations and activities

Enzyme and Microbial Technology

Volumn 20, Issue 6, 1997, Pages 471-477

  Bell, George ^a   Janssen, Anja E M ^b   Halling, Peter J ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Enzyme hydration; Isotherms; Polar solvents; Water activity

Indexed keywords

ACETONE; ACETONITRILE; ALCOHOLS; HYDRATION; ISOTHERMS; ORGANIC SOLVENTS; PHASE EQUILIBRIA; REACTION KINETICS; WATER;

POLAR ORGANIC SOLVENTS; THERMODYNAMIC WATER ACTIVITY; WATER CONCENTRATIONS;

ENZYMES;

ACETONE; ACETONITRILE; ALCOHOL DERIVATIVE; DIOXANE; LACCASE; ORGANIC SOLVENT; TETRAHYDROFURAN; WATER;

ARTICLE; CATALYST; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; ENZYME IMMOBILIZATION; HYDRATION; THERMODYNAMICS; WATER CONTENT;

EID: 0031149286     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(96)00204-9     Document Type: Article

References (19)

1. Tramper, J., Vermue, M. H., Beeftink, H. H., and von Stockar, U. Biocatalysis in Non-conventional Media. Elsevier, Amsterdam, 1992
2. Kvittingen, L. Some aspects of biocatalysis in organic solvents. Tetrahedron 1994, 50, 8253-8274
3. Halling, P. J. Thermodynamic predictions for biocatalysis in nonconventional media: Theory, tests and recommendations for experimental design and analysis. Enzyme Microb. Technol. 1994, 16, 178-206
4. Carrea, G., Ottolina, G., and Riva, S. Role of solvents in the control of enzyme selectivity in organic media. Trends Biotechnol. 1995, 13, 63-70
5. Vermue, M. H. and Tramper, J. Interrelations of chemistry and biotechnology. 5. Biocatalysis in nonconventional media - medium engineering aspects. Pure Appl. Chem. 1995, 67, 345-373
6. Bell, G., Halling, P. J., Moore, B. D., Partridge, J., and Rees, D. G. Biocatalyst behaviour in low-water systems. Trends Biotechnol. 1995, 13, 468-473
7. Valivety, R. H., Halling, P. J., and Macrae, A. R. Reaction rate with lipase catalyst shows similar dependence on water activity in dif-ferent organic solvents. Biochim. Biophys. Acta 1992, 1118, 218-222
8. Svensson, I., Wehtje, E., Adlercreutz, P., and Mattiasson, B. Effects of water activity on reaction rates and equilibrium positions in enzymatic esterifications. Biotechnol. Bioeng. 1994, 44, 549-556
9. Martins, J. F., Desampaio, T. C., Decarvalho, I. B., and Barreiros, S. Lipase-catalyzed esterification of glycidol in nonaqueous solvents - solvent effects on enzymatic activity. Biotechnol. Bioeng. 1994, 44, 119-124
10. Dudal, Y. and Lortie, R. Influence of water activity on the synthesis of triolein catalyzed by immobilized Mucor miehei lipase. Biotechnol. Bioeng. 1995, 45, 129-134
11. van Erp, S. H. M., Kamenskaya, E. O., and Khmelnitsky, Y. L. The effect of water content and nature of organic solvent on enzyme activity in low-water media - a quantitative description. Eur. J. Biochem. 1991, 202, 379-384
12. Gmehling, J. and Onken, U. Vapor-Liquid Equilibrium Data Collection. DECHEMA, Frankfurt, 1977
13. Bell, G., Dolman, M., Halling, P. J., Heßbrügge, B., Moore, B. D., Partridge, J., Rees, D. G., and Vaidya, A. M. Practical methods for measurement and control of water in non-aqueous biocatalysis. Prep. Biotrans., submitted
14. Janssen, A. E. M., van der Padt, A., and van't Riet, K. Prediction of the reaction equilibrium of biocatalytic reactions in aqueous-organic two-phase systems. Biocatal. Biotrans. 1995, 12, 223-240
15. Halling, P. J. High affinity binding of water by proteins is similar in air and in organic solvents. Biochim. Biophys. Acta 1990, 1040, 225-228
16. McMinn, J. H., Sowa, M. J., Charnick, S. B., and Paulaitis, M. E. The hydration of proteins in nearly anhydrous organic solvent suspensions. Biopolymers 1993, 33, 1213-1224
17. Parker, M. C., Moore, B. D., and Blacker, A. J. Measuring enzyme hydration in nonpolar organic solvents using NMR. Biotechnol. Bioeng. 1995, 46, 452-458
18. Borisover, M. D., Sirotkin, V. A., and Solomonov, B. N. Thermodynamics of water binding by human serum-albumin suspended in acetonitrile. Thermochim. Acta 1995, 254, 47-53
19. Valivety, R. H., Halling, P. J., and Macrae, A. R. Water as a competitive inhibitor of lipase-catalysed esterification in organic media. Biotechnol. Lett. 1993, 15, 1133-1138