YIpDCE1 - An integrating plasmid for dual constitutive expression in yeast

Gene

Volumn 212, Issue 2, 1998, Pages 197-202

  Stearman, Robert ^a   Dancis, Andrew ^b   Klausner, Richard D ^a,c  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

ADE2; PGK promoter; Tetrabrid; Two hybrid method

Indexed keywords

IRON; OXIDOREDUCTASE; PERMEASE; PHOSPHATASE; PHOSPHOGLYCERATE KINASE;

ARTICLE; GENE EXPRESSION; GENE INSERTION; GENE LOCUS; IRON TRANSPORT; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE;

BLOTTING, NORTHERN; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CARBOXY-LYASES; CARRIER PROTEINS; CERULOPLASMIN; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; IRON; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PHOSPHOGLYCERATE KINASE; PLASMIDS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSFORMATION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 0032496617     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(98)00179-6     Document Type: Article

References (12)

1. Christianson T.W., Sikorski R.S., Dante M., Shero J.H., Hieter P. Multifunctional yeast high-copy-number shuttle vectors. Gene. 110:1992;119-122.
2. Fields S., Song O.K. A novel genetic system to detect protein-protein interactions. Nature. 340:1989;245-246.
3. Guthrie, C., Fink, G.R. (Eds.), 1991. Guide to Yeast Genetics and Molecular Biology. Methods in Enzymology, vol. 194. Academic Press, San Diego, CA.
4. Kingsman S., Cousens P., Stanway C.A., Chambers A., Wilson M. High-efficiency yeast expression vectors based on the promoter of the phosphogycerate kinase gene. Methods Enzymol. 185:1990;293-341.
5. Osborne M.A., Dalton S., Kochan J.P. The yeast tribrid system - genetic detection of trans-phosphorylated ITAM-SH2-interactions. Biotechnology. 13:1995;1474-1478.
6. Pause A., Lee S., Worrell R.A., Chen D.Y.T., Burgess W.H., Linehan W.M., Klausner R.D. The von Hippel-Linau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins. Proc. Natl. Acad. Sci. USA. 94:1997;2156-2161.
7. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning, A Laboratory Manual, 2nd ed. Cold Spring Harbor Press, Cold Spring Harbor, NY.
8. Sikorski R.S., Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Gentics. 122:1989;19-27.
9. Stearman R., Yuan D.S., Yamaguchi-Iwai Y., Klausner R.D., Dancis A. A permease-oxidase complex involved in high-affinity iron uptake in yeast. Science. 271:1996;1552-1557.
10. Stotz A., Linder P. The ADE2 Gene from Saccharomyces cerevisiae: sequence and new vectors. Gene. 95:1990;91-98.
11. Wodicka L., Dong H., Mittmann M., Ho M.-H., Lockhart D.J. Genome-wide expression monitoring in Saccharomyces cerevisiae. Nat. Biotechnol. 15:1997;1359-1367.
12. Yamaguchi-Iwai Y., Stearman R., Dancis A., Klausner R.D. Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast. EMBO J. 15:1996;3377-3384.