A screen for genes induced in the suprachiasmatic nucleus by light

Science

Volumn 279, Issue 5356, 1998, Pages 1544-1547

  Morris, Mary E ^a   Viswanathan, N ^b   Kuhlman, Sandra ^b   Davis, Fred C ^b   Weitz, Charles J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b NORTHEASTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANIMAL CELL; ARTICLE; CIRCADIAN RHYTHM; EARLY RESPONSE GENE; GENE INDUCTION; GENETIC SCREENING; IMAGE SUBTRACTION; LIGHT DARK CYCLE; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS; SUPRACHIASMATIC NUCLEUS;

ANIMALS; ANTISENSE ELEMENTS (GENETICS); BLOTTING, SOUTHERN; CIRCADIAN RHYTHM; CLONING, MOLECULAR; CRICETINAE; DNA, COMPLEMENTARY; DNA-BINDING PROTEINS; EARLY GROWTH RESPONSE PROTEIN 3; GENE EXPRESSION REGULATION; GENES, FOS; LIGHT; MALE; MESOCRICETUS; POLYMERASE CHAIN REACTION; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECEPTORS, STEROID; RNA, MESSENGER; SUPRACHIASMATIC NUCLEUS; TRANSCRIPTION FACTORS;

ANIMALIA; MAMMALIA; STAPHYLOCOCCUS PHAGE 3A;

EID: 0032489569     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5356.1544     Document Type: Article

References (37)

1. L. N. Edwards, Cellular and Molecular Bases of Bio-logical Clocks (Springer-Verlag, Berlin, 1988).
2. C. S. Pittendrigh and S. Daan, J. Comp. Physiol. 106, 291 (1976).
3. D. C. Klein, R. Y. Moore, S. M. Reppert, Eds., Suprachiasmatic Nucleus: The Mind's Clock (Oxford Univ. Press, New York, 1991).
4. R. Y. Moore and D. C. Klein, Brain Res. 71, 17 (1974); D. C. Klein and R. Y. Moore, ibid. 174, 245 (1979).
5. R. Y. Moore and D. C. Klein, Brain Res. 71, 17 (1974); D. C. Klein and R. Y. Moore, ibid. 174, 245 (1979).
6. R. F. Johnson, R. Y. Moore, L. P. Morin, ibid. 460, 297 (1988).
7. J. S. Takahashi, Annu. Rev. Neurosci. 18, 531 (1995).
8. M. A. Rea, Brain Res. Bull. 23, 577 (1989); B. Rusak, H. A. Robertson, W. Wisden, S. P. Hunt, Science 248, 1237 (1990); J. M. Kernhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Neuron 5, 127 (1990); B. Rusak, L. McNaughton, H. A. Robertson, S. P. Hunt, Mol. Brain Res. 14, 124 (1992); J. M. Kornhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Science 255, 1581 (1992); M. E. Guido, B. Rusak, H. A. Robertson, Brain Res. 739, 132 (1996).
9. M. A. Rea, Brain Res. Bull. 23, 577 (1989); B. Rusak, H. A. Robertson, W. Wisden, S. P. Hunt, Science 248, 1237 (1990); J. M. Kernhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Neuron 5, 127 (1990); B. Rusak, L. McNaughton, H. A. Robertson, S. P. Hunt, Mol. Brain Res. 14, 124 (1992); J. M. Kornhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Science 255, 1581 (1992); M. E. Guido, B. Rusak, H. A. Robertson, Brain Res. 739, 132 (1996).
10. M. A. Rea, Brain Res. Bull. 23, 577 (1989); B. Rusak, H. A. Robertson, W. Wisden, S. P. Hunt, Science 248, 1237 (1990); J. M. Kernhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Neuron 5, 127 (1990); B. Rusak, L. McNaughton, H. A. Robertson, S. P. Hunt, Mol. Brain Res. 14, 124 (1992); J. M. Kornhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Science 255, 1581 (1992); M. E. Guido, B. Rusak, H. A. Robertson, Brain Res. 739, 132 (1996).
11. M. A. Rea, Brain Res. Bull. 23, 577 (1989); B. Rusak, H. A. Robertson, W. Wisden, S. P. Hunt, Science 248, 1237 (1990); J. M. Kernhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Neuron 5, 127 (1990); B. Rusak, L. McNaughton, H. A. Robertson, S. P. Hunt, Mol. Brain Res. 14, 124 (1992); J. M. Kornhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Science 255, 1581 (1992); M. E. Guido, B. Rusak, H. A. Robertson, Brain Res. 739, 132 (1996).
12. M. A. Rea, Brain Res. Bull. 23, 577 (1989); B. Rusak, H. A. Robertson, W. Wisden, S. P. Hunt, Science 248, 1237 (1990); J. M. Kernhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Neuron 5, 127 (1990); B. Rusak, L. McNaughton, H. A. Robertson, S. P. Hunt, Mol. Brain Res. 14, 124 (1992); J. M. Kornhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Science 255, 1581 (1992); M. E. Guido, B. Rusak, H. A. Robertson, Brain Res. 739, 132 (1996).
13. M. A. Rea, Brain Res. Bull. 23, 577 (1989); B. Rusak, H. A. Robertson, W. Wisden, S. P. Hunt, Science 248, 1237 (1990); J. M. Kernhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Neuron 5, 127 (1990); B. Rusak, L. McNaughton, H. A. Robertson, S. P. Hunt, Mol. Brain Res. 14, 124 (1992); J. M. Kornhauser, D. E. Nelson, K. E. Mayo, J. S. Takashashi, Science 255, 1581 (1992); M. E. Guido, B. Rusak, H. A. Robertson, Brain Res. 739, 132 (1996).
14. N. Aronin, S. M. Sagar, F. R. Sharp, W. J. Schwartz, Proc. Natl. Acad. Sci. U.S.A. 87, 5959 (1990); D. D. Ginty et al., Science 260, 238 (1993); F. Wollnik et al., Eur. J. Neurosci. 7, 388 (1995).
15. N. Aronin, S. M. Sagar, F. R. Sharp, W. J. Schwartz, Proc. Natl. Acad. Sci. U.S.A. 87, 5959 (1990); D. D. Ginty et al., Science 260, 238 (1993); F. Wollnik et al., Eur. J. Neurosci. 7, 388 (1995).
16. N. Aronin, S. M. Sagar, F. R. Sharp, W. J. Schwartz, Proc. Natl. Acad. Sci. U.S.A. 87, 5959 (1990); D. D. Ginty et al., Science 260, 238 (1993); F. Wollnik et al., Eur. J. Neurosci. 7, 388 (1995).
17. T. Pavlidis, Biological Oscillators: Their Mathematical Analysis (Academic Press, New York, 1973).
18. N. Lisitsyn, N. Lisitsyn, M. Wigler, Science 259, 946 (1993).
19. Eighty-four adult male Syrian hamsters (Charles River Laboratories) were entrained to a light-dark cycle (14 hours light, 10 hours dark) for 3 weeks. The hamsters were then transferred to constant dim light (<1 lux) for 7 days, and circadian phases were estimated from spontaneous running-wheel activity (2). At CT 19 on the seventh day, half of the hamsters received a light treatment (250 lux, 25 to 35 min), and the other half received a sham treatment (similar handling, but light <1 lux). At the end of the treatment, brains were removed (40 from each group) and placed in phosphate-buffered saline (PBS, 4°C) for 30 s, and a 1.5-mm-thick coronal slice containing the SCN was cut (Stoelting tissue slicer) and transferred to cold PBS. A 1-mm tissue punch (Fine Science Tools USA) containing the SCN was taken, frozen on dry ice, and stored (-7O°C). The time between decapitation and freezing of dissected SCNs was 6 to 7 min. Care of hamsters and all procedures were in full compliance with institutional guidelines for animal experimentation.
20. 1 each digest being used for a separate RDA experiment. Fragments were blunt-ligated on each end to a linker containing a Bgl ll site [top (A-Bgl-24), 5'-TC-CAGCCTCTCACCGCAGATCTGG; bottom, 5'-CCAGATCTGCGGTGAG], and products between 150 and 1500 base pairs were gel-purified; 100 ng of the linked cDNA was used as PCR template in 4 ml of reaction mix, divided into 10 tubes (400 μl each) of PCR buffer (10) containing 1.25 μM A-Bgl-24 (top) primer. Samples were warmed to 72°C for 1 min, Amplitaq (Perkin-Elmer, 37.5 U/ml) was added, and 15 cycles (94°C for 1 min, 72°C for 3 min) were performed. Linkers were removed with Bgl II, and fragments from 150 to 1500 base pairs were gel-purified, generating a cDNA representation of the original mRNA to be used as tester or driver in RDA (10).
21. RDA was performed essentially as described (10), but with the following modifications. Hybridizations were for 40 hours (round 1 : driver, 20 μg, tester, 200 ng; round 2: driver, 20 μg, difference product 1, 20 ng; round 3: driver, 20 μg, difference product 2, 500 fg). After round 3, linkers were removed with Bgl II, and PCR products were ligated into PCRscript (Stratagene). For forward subtractions, the cDNA representation from the sham-treated hamsters was used as the driver and that from light-treated hamsters as the tester (10); for reverse subtractions, the driver and tester designations were reversed.
22. 32P-labeled cDNA was synthesized from final RDA products from the forward and reverse subtractions (13), respectively, and washed as above.
23. Forty-one plasmids that were isolated in control reverse subtractions (13) or that corresponded to highly abundant transcripts were set aside as likely artifacts; nearly half were accounted for by three clones.
24. 35S-labeled riboprobes was as described [D. G. Wilkinson, J. A. Bailes, J. E. Champion, A. P. McMahon, Development 99, 493 (1987)].
25. 35S-labeled riboprobes was as described [D. G. Wilkinson, J. A. Bailes, J. E. Champion, A. P. McMahon, Development 99, 493 (1987)].
26. M. E. Morris, S. Kuhlman, F. C. Davis, C. J. Weitz, unpublished data.
27. -9) were to different segments of the 3' untranslated region of human egr-3, starting, respectively, 0.8 kb and 1.5 kb 3' from the end of the egr-3 coding sequence.
28. S. Patwardhan et al., Oncogene 6, 917 (1991).
29. J. Honkaniemi, J. Kononen, T. Kainu, I. Pyykonen, M. Pelto-Huikko, Mol Brain Res. 25, 234 (1994); J. Honkaniemi, S. M. Sagar, I. Pyykonen, K. J. Hicks, F. R. Sharp, ibid. 28, 157 (1995).
30. J. Honkaniemi, J. Kononen, T. Kainu, I. Pyykonen, M. Pelto-Huikko, Mol Brain Res. 25, 234 (1994); J. Honkaniemi, S. M. Sagar, I. Pyykonen, K. J. Hicks, F. R. Sharp, ibid. 28, 157 (1995).
31. R. Silver et al., Neuroreport 7, 1224 (1996).
32. J. D. Miller, L. P. Morin, W. J. Schwartz, R. Y. Moore, Steep 19, 641 (1996).
33. J. A. Treep, H. Abe, B. Rusak, D. M. Goguen, J. Biol. Rhythms 10, 299 (1995).
34. H. Abe, B. Rusak, H. A. Robertson, Neurosci. Lett. 127, 9 (1991); Brain Res. Bull. 28, 831 (1992).
35. H. Abe, B. Rusak, H. A. Robertson, Neurosci. Lett. 127, 9 (1991); Brain Res. Bull. 28, 831 (1992).
36. Y. Shigeyoshi et al., Cell 91, 1043 (1997).
37. We thank N. Gekakis and D. Staknis for helpful contributions; D. Nathans, M. Greenberg, P. Worley, and A. Lanahan for plasmids; J. Takahashi for a Syrian hamster genomic library; L. Buck for use of her microscope; S. Sullivan for guidance on in situ hybridization; N. Nakanishi and W. Schwartz for helpful suggestions; D. Paul, J. Cohen, and I. Chiu for comments on the manuscript; and L. Ruthig and J. Lee for technical assistance. Supported by a McKnight Scholars Award (C.J.W.), the Council for Tobacco Research-USA, Inc. (C.J.W.), a Stuart H. Q. and Victoria Quan Fellowship in Neurobiology (M.E.M), and NIH (F.C.D.).