Site-directed mutagenesis of the A-factor receptor protein: Val-41 important for DNA-binding and Trp-119 important for ligand-binding

Gene

Volumn 222, Issue 1, 1998, Pages 133-144

  Sugiyama, Masakazu ^a,b   Onaka, Hiroyasu ^a   Nakagawa, Taishiro ^a,c   Horinouchi, Sueharu ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b AJINOMOTO CO INC   (Japan)

^c KIRIN BREWERY CO LTD   (Japan)

Author keywords

Autoregulator; DNA binding protein; Helix turn helix DNA binding motif; Morphogenesis; Secondary metabolism; Streptomyces griseus

Indexed keywords

DNA BINDING PROTEIN; FACTOR A; HELIX LOOP HELIX PROTEIN; RECEPTOR PROTEIN; VALINE;

ARTICLE; GENE EXPRESSION REGULATION; LIGAND BINDING; METABOLISM; MORPHOGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STREPTOMYCES;

4-BUTYROLACTONE; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; DNA-BINDING PROTEINS; GROWTH SUBSTANCES; LIGANDS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; REPRESSOR PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY; STREPTOMYCES; STREPTOMYCES GRISEUS;

ACTINOBACTERIA (CLASS); STREPTOMYCES; STREPTOMYCES COELICOLOR; STREPTOMYCES GRISEUS; UNCULTURED ACTINOMYCETE;

EID: 0032487744     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(98)00487-9     Document Type: Article

References (30)

1. Anisova, L.N., Blinova, I.N., Efremenkova, O.V., Koz'min, Y.P., Onoprienko, V.V., Smirnova, G.M., Khokhlov, A.S., 1984. Regulators of the development of Streptomyces coelicolor A3(2). Izv. Akad. Nauk SSSR, Ser. Biol. 1, 98-108.
2. Bibb, M.J., 1996. The regulation of antibiotic production in Streptomyces coelicolor A3(2). Microbiology 142, 1335-1344.
3. Burnett, W.N., (1981) 'Western blotting': electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112, 195-203.
4. Chater, K.F., 1984. Morphological and physiological differentiation in Streptomyces. In: Losick, R., Shapiro L. (Eds.), Microbial Development. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 89-115.
5. Chater, K.F., 1989. Sporulation in Streptomyces. In: Smith, I., Slepecky, R. A., Setlow, P. (Eds.), Regulation of Procaryotic Development: Structural and Functional Analysis of Bacterial Sporulation and Germination. American Society for Microbiology, Washington, DC, pp. 277-299.
6. Hara, O., Horinouchi, S., Uozumi, T., Beppu, T., 1983. Genetic analysis of A-factor synthesis in Streptomyces coelicolor A3(2) and Streptomyces griseus. J. Gen. Microbiol. 129, 2939-2944.
7. Horinouchi, S., Beppu, T., 1990. Autoregulatory factors of secondary metabolism and morphogenesis in actinomycetes. Crit. Rev. Biotechnol. 10, 191-204.
8. Horinouchi, S., Beppu, T., 1992. Autoregulatory factors and communication in actinomycetes. Annu. Rev. Microbiol. 46, 377-398.
9. Horinouchi, S., Beppu, T., 1994. A-Factor as a microbial hormone that controls cellular differentiation and secondary metabolism in Streptomyces griseus. Mol. Microbiol. 12, 859-864.
10. Horinouchi, S., Kumada, Y., Beppu, T., 1984. Unstable genetic determinant of A-factor biosynthesis in streptomycin-producing organisms: cloning and characterization. J. Bacteriol. 158, 481-487.
11. Khokhlov, A.S., 1988. Results and perspectives of actinomycete autoregulators studies. In: Okami, Y., Beppu, T., Ogawara H. (Eds.), Biology of Actinomycetes '88. Japan Scientific Societies Press, Tokyo, pp. 338-345.
12. Kunkel, T.A., Roberts, J.D., Zakour, R.A., 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367-382.
13. Lamark, T., Kaasen, I., Eshoo, M.W., Falkenberg, P., McDougall, J., Strom, A.R., 1991. DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli. Mol. Microbiol. 5, 1049-1064.
14. Maniatis, T., Fritsch, E.F., Sambrook, J., 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Miyake, K., Horinouchi, S., Yoshida, M., Chiba, N., Mori, K., Nogawa, N., Morikawa, N., Beppu, T., 1989. Detection and properties of A-factor-binding protein from Streptomyces griseus. J. Bacteriol. 171, 4298-4302.
16. Miyake, K., Kuzuyama, T., Horinouchi, S., Beppu, T., 1990. The A-factor-binding protein of Streptomyces griseus negatively controls streptomycin production and sporulation. J. Bacteriol. 172, 3003-3008.
17. Okamoto, S., Nakamura, K., Nihira, T., Yamada, Y., 1995. Virginiae butanolide binding protein from Streptomyces virginiae: evidence that VbrA is not the virginiae butanolide binding protein and reidentification of the true binding protein. J. Biol. Chem. 270, 12319-12326.
18. Onaka, H., Ando, N., Nihira, T., Yamada, Y., Beppu, T., Horinouchi, S., 1995. Cloning and characterization of the A-factor receptor gene from Streptomyces griseus. J. Bacteriol. 177, 6083-6092.
19. Onaka, H., Horinouchi, S., 1997. DNA-binding activity of the A-factor receptor protein and its recognition DNA sequences. Mol. Microbiol. 24, 991-1000.
20. Onaka, H., Nakagawa, T., Horinouchi, S., 1998. Involvement of two A-factor receptor homologues in Streptomyces coelicolor A3(2) in the regulation of secondary metabolism and morphogenesis. Mol. Microbiol. 28, 743-753.
21. Onaka, H., Sugiyama, M., Horinouchi, S., 1997. A mutation at proline-115 in the A-factor receptor protein of Streptomyces griseus abolishes DNA-binding ability but not ligand-binding ability. J. Bacteriol. 179, 2748-2752.
22. Rouch, D.A., Cram, D.S., DiBerardino, D., Littlejohn, T.G., Skurray, R.A., 1990. Efflux-mediated antiseptic resistance gene qacA from Staphylococcus aureus: common ancestry with tetracycline- and sugar-transport proteins. Mol. Microbiol. 4, 2051-2062.
23. Sanger, F., Nicklen, S., Coulson, A.R., 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
24. Schumacher, M.A., Choi, K.Y., Zalkin, H., Brennan, R.G., 1994. Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by a helices. Science 266, 763-770.
25. Schell, M.A., 1993. Molecular biology of the LysR family of transcriptional regulators. Annu. Rev. Microbiol. 47, 597-626.
26. Suckow, J., Markiewicz, P., Kleina, L.G., Miller, J., Kisters-Woike, B., Müller-Hill, B., 1996. Genetic studies of the Lac repressor XV: 4000 single amino acid substitution and analysis of the resulting phenotypes on the basis of the protein structure. J. Mol. Biol. 261, 509-523.
27. Vujaklija, D., Horinouchi, S., Beppu, T., 1993. Detection of an A-factor-responsive protein that binds to the upstream activation sequence of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. J. Bacteriol. 175, 2652-2661.
28. Waki, M., Nihira, T., Yamada, Y., 1997. Cloning and characterization of the gene (farA) encoding the receptor for an extracellular regulatory factor (IM-2) from Streptomyces sp. strain FRI-5. J. Bacteriol. 179, 5131-5137.
29. Weickert, M.J., Adhya, S., 1992. A family of bacterial regulators homologous to Gal and Lac repressors. J. Biol. Chem. 267, 15869-15874.
30. Yanisch-Perron, C., Vieira, J., Messing, J., 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13mp18 and pUC19 vectors. Gene 33, 103-119.