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3543029365
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We have not studied this form systematically, and these "methods" are merely post-hoc explanations of why samples were inadvertently damaged
-
We have not studied this form systematically, and these "methods" are merely post-hoc explanations of why samples were inadvertently damaged.
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-
-
-
28
-
-
3543003036
-
-
note
-
57Feenriched samples of activated a that have been examined by Mössbauer had an average of 35% of A-clusters in the Ni-labile form, and exhibited an average pNiFeC intensity of 0.22 spin/α. In principle, these two numbers (35% and 22%) should be equal. The reason for this discrepancy is not clear, but a similar ratio (22/35 = 0.63) was observed for a native enzyme sample, and thus it seems to be reproducible. The fraction of Ni-labile clusters in a sample is assumed to equal the (pNiFeC spin intensity)/0.63. Since the damaged form seems to arise at the expense of Ni-labile A-clusters, and these two forms together account for ∼50% of the A-clusters in a given sample, the fraction of damaged A-clusters in a sample is ∼0.5 - [(pNiFeC spin intensity)/(0.63)]. The fraction of nonlabile A-clusters is presumed to be 0.5 in all samples.
-
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31
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34
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3543048133
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The EXAFSPAK program was develped by Graham N. George and is available on the SSRL Web site at http://ssr101.slac.stanford.edu/ exafspak.html.
-
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35
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0001661950
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3542997599
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Reference deleted on revision
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Reference deleted on revision.
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39
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3543019252
-
-
note
-
2.
-
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-
-
40
-
-
3543004259
-
-
note
-
The blue color of the methyl viologen/phen/CODH solution disappeared during the experiment, and it returned upon treatment with CO. Thus, reduced methyl viologen did not completely inhibit the removal of Ni, apparently because the solution became oxidized during the course of the reaction.
-
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41
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For further discussion relevant to this assignment see: Qiu et al. J. Am. Chem. Soc. 1997, 119, 11134. Spiro, T. G. Science 1997, 278, 21.
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