α-latrotoxin action probed with recombinant toxin: Receptors recruit α-latrotoxin but do not transduce an exocytotic signal

EMBO Journal

Volumn 17, Issue 21, 1998, Pages 6188-6199

  Ichtchenko, Konstantin ^a,c   Khvotchev, Mikhail ^a   Kiyatkin, Nikita ^b   Simpson, Lance ^a   Sugita, Shuzo ^a   Südhof, Thomas C ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b THOMAS JEFFERSON UNIVERSITY   (United States)

^c NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

latrotoxin; CIRL; Latrophilin; Neurexins; Neurotransmitter release

Indexed keywords

ALPHA LATROTOXIN; CADMIUM; CYSTEINE; GLUTAMATE RECEPTOR; GLUTAMIC ACID; LANTHANUM; MUTANT PROTEIN; PHOSPHOLIPASE C; POLYPHOSPHOINOSITIDE; RECOMBINANT PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; EXOCYTOSIS; HYDROLYSIS; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT; RECEPTOR BINDING; SYNAPTIC TRANSMISSION; TOXIN ANALYSIS;

ANIMALS; CADMIUM; CYSTEINE; EXOCYTOSIS; GLYCOPROTEINS; LANTHANUM; MICE; MUTATION; NERVE TISSUE PROTEINS; NEUROPEPTIDES; NEUROTRANSMITTER AGENTS; PHOSPHATIDYLINOSITOLS; PHOSPHOLIPASE C; PROTEIN BINDING; RECEPTORS, PEPTIDE; RECOMBINANT PROTEINS; SIGNAL TRANSDUCTION; SPIDER VENOMS; SYNAPTOSOMES;

ANIMALIA;

EID: 0032476601     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.21.6188     Document Type: Article

References (34)

1. Capogna,M., Gahwiler,B.H. and Thompson,S.M. (1996) Calcium-independent actions of α-latrotoxin on spontaneous and evoked lynaptic transmission in the hippocampus. J. Newophysiol., 76, 149-158.
2. 2+-dependent recycling of synaptic vesicles at the frog neuromuscular junction. J. Cell Biol., 87, 297-303.
3. Davletov,B.A., Krasnoperov,V., Hata,Y., Petrenko,A.G. and Sudhof,T.C. (1995) High affinity binding of α-latrotoxin to recombinant neurexin Iα. J. Biol. Chem., 270, 23903-23905.
4. 2+-dependent α-latrotoxin-binding protein. J. Biol. Chem., 271, 23239-23245.
5. 2+. EMBO J., 17, 3909-3920.
6. De Camilli,P., Emr,S.D., McPherson,P.S. and Novick,P. (1996) Phosphoinositides as regulators in membrane traffic. Science, 271, 1533-1539.
7. Dulubova,I.E., Krasnoperov,V.G., Khvotchev,M.V., Pluzhnikov,K.A., Volkova,T.M., Grishin,E.G., Vais,H., Bell,D.R. and Usherwood,P.N.R. (1996) Cloning and structure of δ-latroinsectotoxin, a novel insect-specific member of the latrotoxin family. J. Biol. Chem., 271, 7535-7543.
8. Finkelstein,A., Rubin,L.L. and Tzeng,M.C. (1976) Black widow spider venom: effect of purified toxin on lipid bilayer membranes. Science, 193, 1009-1011.
9. Frontali,N. and Grasso,A. (1964) Separation of three toxicologically different protein components from the venom of the spider Latrodectus tredecimguttatus. Arch. Biochem. Biophys., 106, 213-218.
10. Frontali,N., Ceccarelli,B., Gorio,A., Mauro,A., Siekevitz,P., Tzeng, M-C. and Hurlbut,W.P. (1976) Purification from black widow spider venom of a protein factor causing the depletion of synaptic vesicles at neuromuscular junctions. J. Cell Biol., 68, 462-479.
11. Geppert,M., Khvotchev,M., Krasnoperov,V., Goda,Y., Missler,M., Hammer,R.E., Ichtchenko,K., Petrenko,A.G. and Südhof,T.C. (1998) Neurexin Iα is a major α-latrotoxin receptor that cooperates in a α-latrotoxin action. J. Biol. Chem., 273, 1705-1710.
12. Gorio,A., Rubin,L.L. and Mauro,A. (1978) Double mode of action of black widow spider venom on frog neuromuscular junction. J. Neurocytol., 7, 193-202.
13. Grishin,E.V., Himmelreich,N.H., Pluzhnikov,K.A., Pozdnyakova,N.G., Storchak,L.G., Volkova,T.M. and Woll,P.G. (1993) Modulation of functional activities of the neurotoxin from black widow spider venom. FEBS Lett., 336, 205-207.
14. Hata,J., Butz,S. and Südhof,T.C. (1996) CASK: a novel dlg/PSD95 homologue with an N-terminal CaM kinase domain identified by interaction with neurexins. J. Neurosci., 16, 2488-2494.
15. Hurlbut,W.P., Chieregatgti,E., Valtona,F. and Haimann,C. (1994) α- Latrotoxin channels in neuroblastoma cells. J. Membr. Biol., 138, 91-102.
16. Khvotchev,M. and Südhof,T.C. (1998) Newly synthesized phosphatidylinositol phosphates are required for synaptic norepinephrine but not glutamate or GABA release. J. Biol. Chem., 273, 21451-21454.
17. Kiyatkin,N., Dulubova,I.E., Chekhovskaya,I.A. and Grishin,E. (1990) Cloning and structure of cDNA encoding α-latrotoxin from black widow spider venom. FEBS Lett., 270, 127-131.
18. Kiyatkin,N., Dulubova,L. Chekhovskaya,I., Lipkin,A. and Grishin,E. (1992) Structure of the low molecular weight protein copurified with α-latrotoxin. Toxicon, 7, 771-774.
19. Kiyatkin,N., Dulubova,I. and Grishin,E. (1993) Cloning and structural analysis of α-latroinsectotoxin cDNA. Abundance of ankyrin-like repeats. Eur. J. Biochem., 213, 121-127.
20. Kiyatkin,N.I., Kulikovskaya,I.M., Grishin,E.V., Beadle,D.J. and King,L.A. (1995) Functional characterization of black widow spider neurotoxins synthesised in insect cells. Eur. J. Biochem., 230, 854-859.
21. Knipper,M., Madeddu,L., Breer,H. and Meldolesi,J. (1986) Black widow spider venom-induced release of neurotransmitters: mammalian synaptosomes are stimulated by a unique venom component (α-latrotoxin), insect synaptosomes by multiple components. Neuroscience, 19, 55-62.
22. Krasnoperov,V.G. et al. (1997) α-Latrotoxin stimulates exocytosis by the interaction with a neuronal G-protein-coupled receptor. Neuron, 18, 925-937.
23. Lelianova,V.G., Davletov,B.A., Sterling,A., Rahman,M.A., Grishin,E.V., Totty,N.F. and Ushkaryov,YA. (1997) α-Latrotoxin receptor, latrophilin, is a novel member of the secretin family of G protein-coupled receptors. J. Biol. Chem., 272, 21504-21508.
24. Magazanik,L.G., Fedorova,I.M., Kovalevskaya,G.I., Pashkov,V.N., Bulgakow,O.V. and Grishin,E.V. (1992) Selective presynaptic insectotoxin (α-latroinsectotoxin) isolated from black widow spider venom. Neuroscience, 46, 181-188.
25. Massague,J. (1996) TGFβ signaling: receptors, transducers and mad proteins. Cell, 85, 947-950.
26. Missler,M. and Sudhof,T.C. (1998) Neurexins: three genes and 1001 products. Trends Genet., 14, 20-25.
27. 2+ channel complex. FEBS Lett., 326, 255-260.
28. Petrenko,A.G., Perin,M.S., Davletov,B.A., Uhskaryov,Y.A., Geppert,M. and Südhof,T.C. (1991) Binding of synaptotagmin to the α-latrotoxin receptor implicates both in synaptic vesicle exocytosis. Nature, 353, 65-68.
29. Rosahl,T.W., Spillane,D., Missler,M., Herz,J., Selig,D.K., Wolff,J.R., Hammer,R.E., Malenka,R.C. and Sudhof,T.C. (1995) Essential functions of synapsins I and II in synaptic vesicle regulation. Nature, 375, 488-493.
30. Rosenmund,C. and Stevens,C.F. (1996) Definition of the readily releasable pool of vesicles at hippo campai synapses. Neuron, 16, 1197-1207.
31. 2+-independent effects mediated by the toxin. Mol. Pharmacol., 38, 917-923.
32. Tzeng,M.C. and Siekevitz,P. (1979) The binding interaction between α-latrotoxin from black widow spider venom and dog cerebral cortex synaptosomal membrane preparations. J. Neurochem., 33, 263-274.
33. Ushkaryov,Y.A., Petrenko,A.G., Geppert,M. and Südhof,T.C. (1992) Neurexins: synaptic cell surface proteins related to the α-latrotoxin receptor and laminin. Science, 257, 50-56.
34. Vicentini,L.M. and Meldolesi,J. (1984) α-latrotoxin of black widow spider venom binds to a specific receptor coupled to phosphoinositide breakdown in PC12 cells. Biochem. Biophys. Res. Commun., 121, 538-544.