Two-step cycle sequencing reduces premature terminations when using primers with high annealing temperatures

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 10, Issue 3, 1998, Pages 231-236

  Prychitko, Thomas Michael ^a   Ries, Elizabeth Ann ^b   Moore, William Samuel ^b  

^a WAYNE STATE UNIVERSITY   (United States)

^b NONE

Author keywords

Automated DNA sequencing; Cycle sequencing; Premature terminations; Primers; Secondary structure

Indexed keywords

DNA POLYMERASE;

ARTICLE; AUTOMATION; COST EFFECTIVENESS ANALYSIS; DNA TEMPLATE; GENE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN SECONDARY STRUCTURE; SEQUENCE ANALYSIS; TEMPERATURE MEASUREMENT;

EID: 0032464561     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02740843     Document Type: Article

References (12)

1. Gibbs, R. A., Nguyen, P. N., McBride, L. J., Koepf, S. M., and Caskey, C. T. (1989) Identification of mutations leading to Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA. Proc. Natl. Acad. Sci. USA 86, 1919-1923.
2. Hattori, M., Shibata, A., Yoshioka, K., and Sakaki, Y. (1993) A novel method of detecting point mutations using an automated fluorescence DNA sequencer. Genomics 15, 415-417.
3. Leren, T. P., Rodningen, O. K., Rosby, O., Solberg, K., and Berg, K. (1993) Screening for point mutations by semi-automated DNA sequencing using sequenase and magnetic beads. BioTechniques 14, 618-623.
4. Parker, L. T., Deng, Q., Zakeri, H., Carlson, C., Nickerson, D. A., and Kwok, P. Y. (1995) Peak height variations in automated sequencing of PCR products using Taq dye-terminator chemistry. BioTechniques 19, 116-121.
5. Kwok, P. Y., Carlson, C., Yager, T. D., Ankener, W., and Nickerson, D. A. (1994) Comparative analysis of human DNA variations by fluorescence-based sequencing of PCR products. Genomics 23, 138-144.
6. Lee, L. G., Connell, C. R., Woo, S. L., Cheng, R. D., McArdle, B. F., Fuller, C. W., et al. (1992) DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effects of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments. Nucleic Acids Res. 20, 2471-2483.
7. Rosenthal, A. and Charnock-Jones, D. S. (1992) New protocols for DNA sequencing with dye terminators. DNA Sequence 3, 61-64.
8. Tracy, T. E. and Mulcahy, L. S. (1991) A simple method for direct automated sequencing of PCR fragments. BioTechniques 11, 68-75.
9. Olsen, D. B. and Eckstein, F. (1989) Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing. Nucleic Acids Res. 17, 9613-9620.
10. Tombline, G., Bellizzi, D., and Sgaramella, V. (1996) Heterogeneity of primer extension products in asymmetric PCR is due both to cleavage by a structure-specific exo/endonuclease activity of DNA polymerases and to premature stops. Proc. Natl. Acad. Sci. USA 93, 2724-2728.
11. Prychitko, T. M. and Moore, W. S. (1997) Utility of DNA sequences of an intron from the β-fibrinogen gene in phylogenetic analysis of woodpeckers (Aves: Picidae). Mol. Phylogenet. Evolution 8, 193-204.
12. Varani, G. (1995) Exceptionally stable nucleic acid hairpins. Annu. Rev. Biophys. Biomol. Struct. 24, 379-404.