Estimation of phosphorus and sulphur contents in nuclear constituents by means of image-EELS analysis using ribosomes as internal standards

Micron

Volumn 29, Issue 6, 1998, Pages 461-468

  Abolhassani Dadras, S ^a   Mayer, J ^b   Fakan, S ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b MAX PLANCK INSTITUTE FOR INTELLIGENT SYSTEMS   (Germany)

Author keywords

Balbiani ring granules; EELS; EFTEM; Electron energy loss spectroscopic imaging; ESI; Image EELS; Phosphorus mapping; Ribonucleoprotein (RNP) structures; Ribosomes; Sulphur mapping

Indexed keywords

EID: 0032452957     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(98)00022-5     Document Type: Article

References (24)

1. Abolhassani-Dadras S., Vázquez-Nin G.H., Echeverría O.M., Fakan S. Image-EELS for in situ estimation of the phosphorus content of RNP granules. J.Microsc. 183(3):1996;215-222.
2. Ahn, C. C. and Krivanek, O. L. (1983) EELS Atlas, p. 16. Centre for Solid State Science, Arizona State University, USA.
3. Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K. and Watson, J. D. (1994) Molecular Biology of the Cell, 3rd edn, pp. 378-379. Garland, New York.
4. Beckers A.L.D., Gelsema E.S., De Bruijn W.C., Cleton-Soeteman M.I., Van Eijk H.G. Quantitative electron spectroscopic imaging in bio-medicine: evaluation and application. J.Microsc. 183(1):1996;78-88.
5. 4. Ultramicroscopy. 55:1994;101-112.
6. Ciliax B.J., Kirk K.L., Leapman R.D. Radiation damage of fluorinated organic compounds measured by parallel electron energy loss spectroscopy. Ultramicroscopy. 48:1993;13-25.
7. Egerton, R. F. (1986) Electron Energy-Loss Spectroscopy in the Electron Microscope, pp. 255-262. Plenum, New York.
8. Egerton, R. F. and Leapman, R. D. (1995) Quantitative electron energy-loss spectroscopy. In Energy Filtering Transmission Electron Microscopy. Springer Series in Optical Sciences, ed. L. Reimer, Vol. 71, pp. 269-290. Springer, Berlin.
9. Körtje K.-H. Image-EELS: simultaneous recording of multiple electron energy-loss spectra from series of electron spectroscopic images. J.Microsc. 174(3):1994;149-159.
10. Krivanek O.L., Mooney P.E. Application of slow-scan CCD cameras in transmission electron microscopy. Ultramicroscopy. 49:1993;95-108.
11. Leapman, R. D., Fiori, C. E. and Swyt, C. R. (1984) Mass thickness determination by inelastic scattering in microanalysis of organic samples. In Analytical Electron Microscopy, eds D. B. Williams and D. C. Joy, pp. 83-88. San Francisco Press, San Francisco.
12. Leapman R.D., Andrews S.B. Biological electron energy loss spectroscopy: the present and the future. Microsc. Microanal. Microstruct. 2:1991;387-394.
13. Leapman R.D., Hunt J.A., Buchanan R.A., Andrews S.B. Measurement of low calcium concentrations in cryosectioned cells by parallel-EELS mapping. Ultramicroscopy. 49:1993;225-234.
14. Martin J.M., Lavergne J.L., Vacher B., Inoue K. Interactive image-spectrum EELS: Application to elemental mapping of lubricant colloids. Microsc. Microanal. Microstruct. 6:1995;53-63.
15. Martin-Perez J., Thomas G. Ordered phosphorylation of 40S ribosomal protein S6 after serum stimulation of quiescent 3T3 cells. Proc. Nat. Acad. Sci. USA. 80:1983;926-930.
16. Mayer J., Plitzko J. Mapping of ELNES on a nanometre scale by electron spectroscopic imaging. J. Microscopy. 183:1996;2-8.
17. Reimer, L. (1991) Energy-filtering transmission electron microscopy. In Advances in Electronics and Electron Physics, ed. P. W. Hawkes, Vol. 81, pp. 43-126. Academic Press, Boston.
18. Reimer, L. (1995) Energy filtering transmission electron microscopy. In Springer Series in Optical Sciences, ed. P. W. Hawkes, Vol. 71, Springer, Berlin.
19. Ross, J. (1995) The Image Processing Handbook, 2nd edn. CRC Press, Ann Arbor, pp. 24-29.
20. Thomas G., Siegmann M., Gordon J. Multiple phosphorylation of ribosomal protein S6 during transition of quiescent 3T3 cells into early G1 and cellular compartment of the phosphate donor. Proc. Nat. Acad. Sci. USA. 76:1979;3952-3956.
21. Vázquez-Nin G.H., Echeverría O.M., Fakan S., Leser G., Martin T.E. Immunoelectron microscope localization of snRNPs in the polytene nucleus of salivary glands of Chironomus thummi. Chromosoma. 99:1990;44-51.
22. Vázquez-Nin G.H., Echeverría O.M., Boutinard Rouelle-Rossier V., Fakan S. A new type of ribonucleoprotein constituent of the polytene nucleus of the salivary glands of Chironomus thummi and Ch. tentans. Chromosoma. 102:1993;693-699.
23. Wang Y.Y., Ho R., Shao Z., Somlyo A.P. Optimization of quantitative electron energy loss spectroscopy in the low loss region: phosphorus L-edge. Ultramicroscopy. 41:1992;11-31.
24. Wurtz T., Lönnroth A., Ovchinnikov L., Skoglund U., Daneholt B. Isolation and initial characterization of a specific premessenger ribonucleoprotein particle. Proc. Nat. Acad. Sci. USA. 87:1990;831-835.