Expression, purification, and characterization of the carboxyl-terminal region of the Na+/H+ exchanger

Biochemistry and Cell Biology

Volumn 76, Issue 5, 1998, Pages 837-842

  Gebreselassie, Daniel ^a   Rajarathnam, Krishna ^a   Fliegel, Larry ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Circular dichroism; Membrane protein; Na+ H+ exchanger; pH regulation

Indexed keywords

ANTIPORTER; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN;

CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CONFERENCE PAPER; CONTROLLED STUDY; GENETIC ENGINEERING; NONHUMAN; PROMOTER REGION; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTON SODIUM EXCHANGE; STRUCTURE ANALYSIS;

CIRCULAR DICHROISM; CLONING, MOLECULAR; ESCHERICHIA COLI; GENETIC TECHNIQUES; HUMANS; HYDROGEN-ION CONCENTRATION; MEMBRANE PROTEINS; RECOMBINANT FUSION PROTEINS; SODIUM-HYDROGEN ANTIPORTER;

ESCHERICHIA COLI;

EID: 0032452187     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/bcb-76-5-837     Document Type: Conference Paper

References (14)

1. Chen, G.-Q., and Gouaux, J.E. 1996. Overexpression of bacterioopsin in Escherichia coli as a water-soluble fusion to maltose binding protein: efficient regeneration of the fusion protein and selective cleavage with trypsin. Protein Sci. 5: 456-467.
2. + exchanger. Edited by L. Fliegel. Springer - R.G. Landes Company, pp. 1-20.
3. Guan, K., and Dixon, J.E. 1991. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192: 262-267.
4. + exchanger. Biochem. J. 289: 637-640.
5. Hjerten, S., Sparrman, M., and Liao, J.-L. 1988. Purification of membrane proteins in SDS and subsequent renaturation. Biochim. Biophys. Acta, 939: 476-484.
6. + exchangers of mammalian cells. J. Biol. Chem. 272: 22373-22376.
7. Perczel, A., Park, L., and Fasman, G.D. 1992. Analysis of the circular dichroism spectrum of proteins using the convex constraint algorithm: a practical guide. Anal. Biochem. 203: 83-93.
8. + exchanger. Edited by L. Fliegel. Springer - R.G. Landes Company, pp. 149-170.
9. + exchanger interacts with mammalian heat shock protein. Biochemistry, 34: 10412-10420.
10. Tuszynski, G.P., and Warren, L. 1975. Removal of sodium dodecyl sulfate from proteins. Anal. Biochem. 67: 55-65.
11. Visser, L., and Blout, E.R. 1971. Elastase. II. Optical properties and the effects of sodium dodecyl sulfate. Biochemistry, 10: 743-752.
12. + exchanger NHE1 through sequence specific interaction. Biochemistry, 36: 12854 -12861.
13. Wang, H., McConnell, D.J., and O'Mahony, D.J. 1990. An efficient temperature-inducible vector incorporating the T7 gene 10 translation initiation leader region. Nucleic Acids Res. 18: 1070.
14. Wishart, D.S., Boyko, R.F., Willard, L., Richards, F.M., and Sykes, B.D. 1994. SEQSEE: a comprehensive program suite for protein sequence analysis. Comp. Applic. Biosci. 10: 121-132.