Large-scale recovery of recombinant protein inclusion bodies expressed in Escherichia coli

Journal of Microbiology and Biotechnology

Volumn 6, Issue 4, 1996, Pages 225-231

  Middelberg, Anton P J ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Cell disruption; Centrifuge; Escherichia coli; Inclusion body; Protein refolding; Recombinant protein

Indexed keywords

EID: 0000658299     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (53)

1. Agerkvist, I. and S.-O. Enfors. 1990. Characterization of E. coli cell disintegrates from a bead mill and high-pressure homogenisers. Biotechnol. Bioeng. 36: 1083-1089.
2. Babbitt, P. C., B. L. West, D. D. Buechter, I. D. Kuntz, and G. L. Kenyon. 1990. Removal of a proteolytic activity associated with aggregates formed from expression of creatine kinase in Escherichia coli leads to improved recovery of active enzyme. Bio/Technol. 8: 945-949.
3. Bailey, S. M., P. H. Blum, and M. Meagher. 1995. Improved homogenization of recombinant Escherichia coli following pretreatment with guanidine hydrochloride. Biotechnol. Prog. 11: 533-539.
4. Chang, J. Y. and J. R. Swartz. 1993. Single-step solubilization and folding of IGF-1 aggregates from Escherichia coli, p. 178-188. In J. L. Cleland (ed.), Protein folding: in vivo and in vitro, ACS Symposium Series 526, American Chemical Society, Washington DC.
5. Chaudhuri, J. B. 1994. Refolding recombinant proteins: process strategies and novel approaches, p. 374-385. In R. K. Bajpai and A. Prokop (eds.), Recombinant DNA Technology II, Annals NY Academy of Sciences, 721, NY Academy of Sciences, NY, NY.
6. Datar, R. V., T. Catrwright, and C.-G. Rosen. 1993. Process economics of animal cell and bacterial fermentations: a case study analysis of tissue plasminogen activator. Bio/ Technol. 11: 349-357.
7. Falconer, R., J. E. Jarvinen, G. Francis, B. K. O'Neill, and A. P. J. Middelberg. 1994. Improvements to a process for producing insulin-like growth factors, p. 384-386. In W. K. Teo, M. G. S. Yap, and S. K. W. Oh (eds.), Better Living Through Innovative Biochemical Engineering, Proceedings of APBioChEC '94, Third Asia-Pacific Biochemical Engineering Conference, Singapore.
8. Falconer, R. J., B. K. O'Neill, and A. P. J. Middelberg. 1996. Chemical permeabilisation of Escherichia coli: an alternative to mechanical disruption. In, Proceedings of the 1996 World Congress of Chemical Engineering (San Diego, USA). American Institution of Chemical Engineers, in press.
9. Fischer, B., I. Sumner, and P. Goodenough. 1993. Isolation, renaturation, and formation of disulfide bonds of eukaryotic proteins expressed in Escherichia coli as inclusion bodies. Biotechnol. Bioeng. 41: 3-13.
10. Forman, S. M., E. R. DeBernardez, R. S. Feldberg, and R. W. Swartz. 1990. Crossflow filtration for the separation of inclusion bodies from soluble proteins in recombinant Escherichia coli cell lysate. J. Membrane Sci. 48: 263-279.
11. Grodberg, J. and J. J. Dunn. 1988. ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification. J. Bacteriol. 170: 1245-1253.
12. 3-IGF-I. p. 250-252. In, Proceedings of 1994 IChemE Research Event (University College London, London, U.K.), Institution of Chemical Engineers, Rugby, U.K.
13. Hart, R. A., U. Rinas, and J. E. Bailey. 1990. Protein composition of Vitreoscilla hemoglobin inclusion bodies produced in Escherichia coli. J. Biol. Chem., 265: 12728-12733.
14. Hart, R. A. and J. E. Bailey. 1992. Solubilization and regeneration of Vitreoscilla hemoglobin isolated from protein inclusion bodies. Biotechnol. Bioeng. 39: 1112-1120.
15. Hart, R. A., P. M. Lester, D. H. Reifsnyder, J. R. Ogez, and S. E. Builder. 1994. Large scale, in situ isolation of periplasmic IGF-I from E. coli. Bio/Technol. 12: 1113-1117.
16. Hart, R. A., J. R. Ogez, and S. E. Builder. 1995. Use of multifactorial analysis to develop aqueous two-phase systems for isolation of non-native IGF-I. Bioseparation 5: 113-121.
17. Jin, K. 1992. Studies of the scale-up of production and recovery of recombinant proteins formed as inclusion bodies. Ph. D. thesis, University of London.
18. Jin. K., O. R. T. Thomas, and P. Dunnill. 1994. Monitoring recombinant inclusion body recovery in an industrial disc stack centrifuge. Biotechnol. Bioeng. 43: 455-460.
19. Kane, J. F. and D. L. Hartley. 1988. Formation of recombinant protein inclusion bodies in Escherichia coli. Tibtech 6: 95-101.
20. Kaufmann, A., Y.-D. Stierhof, and U. Henning. 1994. New outer membraneassociated protease of Escherichia coli K-12. J. Bacteriol. 176: 359-367.
21. Keshavarz-Moore, E., R. Olbrich, M. Hoare, and P. Dunnill. 1991. Application of biochemical engineering principles to develop a recovery process for protein inclusion bodies, p. 307-314. In A. Prokop and R. K. Bajpai (eds.), Recombinant DNA Technology I, Annals NY Academy of Sciences, 646, NY Academy of Sciences, NY, NY.
22. Kotlarski, N., R. S. Yeates, S. J. Milner, G. L. Francis, B. K. O'Neill, and A. P.J. Middelberg. 1995. Studies into the scale-up of a process to produce biosynthetic insulin-like growth factor. Trans. I. Chem. E., part C, 73: 27-32.
23. Krueger, J. K., M. H. Kulke, C. Schutt, and J. Stock. 1989. Protein inclusion body formation and recovery. BioPharm March 1989. p. 40-45.
24. Lazdunski, A. M. 1989. Peptidases and proteases of Escherichia coli and Salmonella typhimurium. FEMS Microbiol. Rev. 63: 265-276.
25. Mannweiler, K. 1989. The recovery of biological particles in high-speed continuous centrifuges with special reference to feed-zone break-up effects. Ph. D. thesis, University of London.
26. Marston, F. A. O. 1986. The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochem. J. 240: 1-12.
27. Meagher, M. M., R. T. Barlett, V. R. Rai, and F. R. Khan. 1994. Extraction of rIL-2 inclusion bodies from Escherichia coli using cross-flow filtration. Biotechnol. Bioeng. 43: 969-977.
28. Middelberg, A. P. J., I. D. L. Bogle, and M. Snoswell. 1989. Simulation of a novel biochemical process. p. 671-678. In, Proceedings of Chemeca '89 (Broadbeach, Qld), Australian Chemical Engineering Conference, Institution of Engineers Australia, Canberra, Australia.
29. Middelberg, A. P. J., I. D. L. Bogle, and M. Snoswell. 1990. Sizing biological samples by photosedimentation techniques. Biotechnol. Prog. 6: 255-261.
30. Middelberg, A. P. J., B. K. O'Neill, and I. D. L. Bogle. 1991. Modelling bioprocess interactions for optimal design and operating strategies, p. 706-712. In, Proceedings of Chemeca '91 (Newcastle, NSW), Australian Chemical Engineering Conference, Institution of Engineers Australia, Canberra, Australia.
31. Middelberg, A. P. J. and B. K. O'Neill. 1991. Monitoring the centrifugal recovery of recombinant protein inclusion bodies. Aust J. Biotech. 5: 87-92.
32. Middelberg, A. P. J., B. K. O'Neill, I. D. L. Bogle, and M. Snoswell. 1991. A novel technique for the measurement of disruption in high-pressure homogenization: studies on E. coli containing recombinant inclusion bodies. Biotechnol. Bioeng. 38: 363-370.
33. Middelberg, A. P. J., B. K. O'Neill, and I. D. L. Bogle. 1992. Modelling bioprocess interactions for optimal design and operating strategies. Trans. I. Chem. E., part C, 70: 8-12.
34. Middelberg, A. P. J., N. Korlarski, and B. K. O'Neill. 1995. The optimisation of biochemical processes involving protein refolding with competing proteolysis. p. 1160-1162. In, Proceedings of 1995 IChemE Research Event (Edinburgh, U.K.), Institution of Chemical Engineers, Rugby, U.K.
35. Middelberg, A. P. J. 1995. The importance of accounting for bioprocess interactions. Aust. Biotechnol. 5: 99-104.
36. Middelberg, A. P. J. 1996. The influence of protein refolding strategy on cost for competing reactions. Biochem. Eng. J. 61: 41-52.
37. Mitraki, A. and J. King. 1989. Protein folding intermediates and inclusion body formation. Bio/Technol., 7: 690-697.
38. Olbrich, R. 1989. The characterisation and recovery of protein inclusion bodies from recombinant Escherichia coli. Ph. D. thesis, University of London.
39. Petrides, D., C. L. Cooney, L. B. Evans, R. P. Field, and M. Snoswell. 1989. Bioprocess simulation: an integrated approach to process development. Computers chem. Engng 13: 553-561.
40. Petrides, D., E. Sapidou, and J. Calandranis. 1995. Computer-aided process analysis and economic evaluation for biosynthetic human insulin production-a case study. Biotechnol. Bioeng. 48: 529-541.
41. Schein, C. H. 1989. Production of soluble recombinant proteins in bacteria. Bio/Technol. 7: 1141-1149.
42. Schuler, M. L. and F. Kargi. 1992. Bioprocess engineering: basic concepts, p. 395-430. Prentice Hall, New Jersey.
43. Sugimura, K. and N. Higashi. 1988. A novel outer-membrane associated protease in Escherichia coli. J. Bacteriol. 170: 3650-3654.
44. Sugimoto, S., Y. Yokoo, Y. Inui, and T. Hirano. 1991. Large-scale purification and refolding of recombinant eel growth hormone. Agric. Biol. Chem., 55: 1635-1637.
45. Lee, S. Y. 1996. High cell-density culture of Escherichia coli. Tibtech 14: 98-104.
46. Taylor, G., M. Hoare, D. R. Gray, and F. A. O. Marston. 1986. Size and density of protein inclusion bodies. Bio/ Technol 4: 553-557.
47. Titchener-Hooker, N. J., D. Gritsis, K. Mannweiler, R. Olbrich, S. A. M. Gardiner, N. M. Fish, and M. Hoare. 1991. Integrated process design for producing and recovering proteins from inclusion bodies. BioPharm July/August 1991, p. 34-38.
48. Valax, P. and G. Georgiou. 1993. Molecular characterisation of β-lactamase inclusion bodies produced in Escherichia coli. 1. Composition. Biotechnol. Prog. 9: 539-547.
49. Vicik, S. and E. DeBernardez-Clark. 1991. An engineering approach to achieving high-protein refolding yields, p. 180-196. In G. Georgiou and E. DeBernardez-Clark (eds.), Refolding, ACS Symposium Series 470, American Chemical Society, Washington DC.
50. Walker, S. G. and A. Lyddiatt. 1996. An investigation of the role for aqueous two phase systems in the processing of α-glucosidase PI inclusion bodies expressed in recombinant E. coli. p. 55-57. In, Proceedings of 1996 IChemE Research Event (Leeds, U.K.), Institution of Chemical Engineers, Rugby, U.K.
51. Wilkinson, D. L. and R. G. Harrison. 1991. Predicting the solubility of recombinant proteins in Escherichia coli. Bio/ Technol. 9: 443-448.
52. Wong, H. H., B. K. O'Neill, and A. P. J. Middelberg. 1996. Centrifugal recovery and dissolution of recombinant Gly-IGF-II inclusion bodies: the impact of feedrate and re-centrifugation on protein yield. Bioseparation, in press.
53. Wong, H. H., B. K. O'Neill, and A. P. J. Middelberg. 1996. The interaction between inclusion body recovery by centrifugation and dissolution, p. 497-501. In, Proceedings of 7th Congress of Asian Pacific Confederation of Chemical Engineers (Taipaǐ, Taiwan, ROC).