In situ immunodetection of activated caspase-3 in apoptotic neurons in the developing nervous system

Cell Death and Differentiation

Volumn 5, Issue 12, 1998, Pages 1004-1016

  Srinivasan, Anu ^a   Roth, Kevin A ^b   Sayers, Robert O ^a   Shindler, Kenneth S ^b   Wong, Angela M ^a   Fritz, Lawrence C ^a   Tomaseili, Kevin J ^a  

^a PFIZER INC   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Apoptosis; Caspase 3; Immunostaining; Neuron; Programmed cell death

Indexed keywords

CASPASE 3; ENZYME PRECURSOR; POLYCLONAL ANTIBODY; PROTEIN BCL X;

ANTIBODY LABELING; APOPTOSIS; ARTICLE; BRAIN CELL; ENZYME ACTIVATION; ENZYME SUBUNIT; HUMAN; HUMAN CELL; MOUSE; NERVOUS SYSTEM DEVELOPMENT; NONHUMAN; PRIORITY JOURNAL;

ORYCTOLAGUS CUNICULUS;

EID: 0032440766     PISSN: 13509047     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cdd.4400449     Document Type: Article

References (21)

1. Armstrong RC, Aja T, Xiang J, Gaur S, Krebs JF, Hoang K, Bai X, Korsmeyer SJ, Karanewsky DS, Fritz LC and Tomaselli KJ (1996) Fas-induced activation of the cell death-related protease CPP32 is inhibited by Bcl-2 and by ICE family protease inhibitors. J. Biol. Chem. 271: 16850 -16855
2. Armstrong RC, Aja TJ, Hoang KD, Gaur S, Bai X, Alnemri ES, Litwack G, Karanewsky DS, Fritz LC and Tomaselli KJ (1997) Activation of the CED3/ICE-related protease CPP32 in cerebellar granule neurons undergoing apoptosis but not necrosis. J. Neurosci. 17: 553-562
3. Bossy-Wetzel E, Newmeyer DD and Green D (1998) Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD-specific caspase activation and independently of mitochondrial transmembrane depolarization. EMBO J. 17: 37-49
4. Chinnaiyan AM, Orth K, O'Rourke K, Duan H, Poirier GG and Dixit VM (1996) Molecular ordering of the cell death pathway. J. Biol. Chem. 271: 4573-4576
5. Cohen GM (1997) Casapses: the executioners of apoptosis. Biochem. 326: 1-16
6. Deckwerth TL, Elliott JL, Knudson CM, Johnson Jr EM, Snider WD and Korsmeyer SJ (1996) BAX is required for neuronal death after trophic factor deprivation and during development. Neuron 17: 401-411
7. Dessi F, Pollard H, Moreau J, Ben-Ari Y, Charriaut-Martangue C (1995) Cytosine arabinoside induces apoptosis in cerebellar neurons in culture. J. Neurochem. 64: 1980-1987
8. Farlie PG, Dringen R, Rees SM, Kannourakis G and Bernard O (1995) bcl-2 transgene expression can protect neurons against developmental and induced cell death. Proc. Natl. Acad. Sci. USA. 92: 4397-4401
9. Frankowski H, Missotten M, Fernandez P-A, Martinou I, Michel P, Sadoul R and Martinou J-C (1995) Function and expression of the Bcl-xgene in the developing and adult nervous system. NeuroReport 6: 1917-1921
10. Garcia I, Martinou I, Tsujimoto Y and Martinou J-C (1992) Prevention of programmed cell death of sympathetic neurons by the bcl-2 proto-oncogene. Science 258: 302-304
11. Gonzalez-Garcia M, Garcia I, Ding L, O'Shea S, Boise LH, Thompson CB and Nunez G (1995) bcl-x is expressed in embryonic and postnatal neural tissues and functions to prevent neuronal cell death. Proc. Natl. Acad. Sci. USA. 92: 4304-4308
12. Greenlund US, Korsmeyer SJ and Johnson Jr EM (1995) Role of BCL-2 in the survival and function of developing and mature sympathetic neurons. Neuron 15: 649-661
13. Harlow E and Lane D (1988) Antibodies: a laboratory manual, pp 285-317, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
14. Henkart PA (1996) ICE family proteases: mediators of all apoptotic cell death? Immunity 4: 195-201
15. Ishitani R and Chuang D-M (1996) Glyceraldehyde-3-phosphate dehydrogenase antisense oligodeoxynucleotides protect against cytosine arabinonucleoside-induced apoptosis in cultured cerebellar neurons. Proc. Natl. Acad. Aci. 93:-9937-9941
16. Keane RW, Srinivasan A, Foster LM, Testa M-P, Ord T, Nonner D, Wang H-G, Reed JC, Bredesen DE and Kayalar C (1997) Activation of CPP32 during apoptosis of neurons and astrocytes. J. Neuroscience Res. 48: 168-180
17. Kluck RM, Bossy-Wetzel E, Green D and Newmeyer DD (1997) The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. Science 275: 1132-1136
18. Kuida K, Zheng TS, Na S, Kuan C-Y, Yang D, Karasuyama H, Rakic P and Flavell RA (1996) Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature 384: 368-372
19. Li F, Srinivasan A, Wang Y, Armstrong RC, Tomaselli KJ and Fritz LC (1997a) Cell-specific induction of apoptosis by microinjection of cytochrome c. J. Biol. Chem. 272: 30299-30305
20. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES and Wang X (1997b) Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91: 479-489
21. Lowry OH, Rosebrough NJ, Farr ALand Randall RJ (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275