Transcriptional regulation and organization of the dcuA and dcuB genes, encoding homologous anaerobic C4-dicarboxylate transporters in Escherichia coli

Journal of Bacteriology

Volumn 180, Issue 24, 1998, Pages 6586-6596

  Golby, Paul ^a   Kelly, David J ^b   Guest, John R ^b   Andrews, Simon C ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BETA GALACTOSIDASE; DICARBOXYLIC ACID;

ARTICLE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GRAM NEGATIVE BACTERIUM; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA;

EID: 0032436622     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.24.6586-6596.1998     Document Type: Article

References (45)

1. Aiba, H., S. Adkya, and B. de Crombrugghe. 1981. Evidence for a functional gal promoter in intact Escherichia coli cells. J. Biol. Chem. 256:11905-11910.
2. Baker, K. E., K. P. Ditullio, J. Neuhard, and R. A. Kelln. 1996. Utilization of orotate as a pyrimidine source by Salmonella typhimurium and Escherichia coli requires the dicarboxylate transport protein encoded by dctA. J. Bacteriol. 178:7099-7105.
3. Bell, J. B., S. A. Andrews, M. N. Sivak, and J. R. Guest. 1989. Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12. J. Bacteriol. 171:3494-3503.
4. Bongaerts, J., S. Zoske, U. Weidner, and G. Unden. 1995. Transcriptional regulation of the proton translocating NADH dehydrogenase genes (nuoA-N) of Escherichia coli by electron acceptors, electron donors and gene regulators. Mol. Microbiol. 6:521-534.
5. Bott, M., M. Meyer, and P. Dimroth. 1995. Regulation of anaerobic citrate metabolism in Klebsiella pneumoniae. Mol. Microbiol. 18:533-546.
6. Cronan, J. E., Jr., and D. LaPorte. 1996. Tricarboxylic acid cycle and glyoxylate bypass, p. 206-216. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaecther, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C.
7. Darwin, A. J., K. L. Tyson, S. J. W. Busby, and V. Stewart. 1997. Differential regulation by the homologous response regulators NarL and NarP of Escherichia coli K12 depends on DNA binding site arrangement. Mol. Microbiol. 25:583-595.
8. Engel, P., R. Kramer, and G. Unden. 1992. Anaerobic fumarate transport in Escherichia coli by an fnr-dependent dicarboxylate uptake system which is different from aerobic dicarboxylate uptake. J. Bacteriol. 174:5533-5539.
9. 4-dicarboxylates by anaerobically grown Escherichia coli: energetics and mechanism of uptake and efflux. Eur. J. Biochem. 222:605-614.
10. 4-dicarboxylate transporter of Escherichia coli. J. Bacteriol. 180:4821-4827.
11. Guest, J. R., J. Green, A. S. Irvine, and S. Spiro. 1996. The FNR modulon and FNR-regulated gene expression, p. 317-342. In E. C. C. Lin and A. S. Lynch (ed.), Regulation of gene expression in Escherichia coli. Landes Co. and Chapman & Hall, Washington, D.C.
12. Guest, J. R., J. S. Miles, R. E. Roberts, and S. A. Woods. 1985. The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC). J. Gen. Microbiol. 131:2971-2984.
13. Guest, J. R., R. E. Roberts, and R. J. Wilde. 1984. Cloning of the aspartase gene (aspA) of Escherichia coli. J. Gen. Microbiol. 130:1271-1278.
14. Iuchi, S., and E. C. Lin. 1987. The narL gene product activates the nitrate reductase operon and represses the fumarate reductase TMAO operons in Escherichia coli. Proc. Natl. Acad. Science USA 84:3901-3905.
15. Jennings, M. P., and I. R. Beacham. 1993. Co-dependent positive regulation of the ansB promoter of Escherichia coli by CRP and the FNR protein - a molecular analysis. Mol. Microbiol. 1:155-164.
16. Jerlström, P. G., J. Liu, and I. R. Beacham. 1987. Regulation of Escherichia coli L-asparaginase II and L-aspartase by the fnr gene-product. FEMS Microbiol. Lett. 41:127-130.
17. Jones, H. M., and R. P. Gunsalus. 1987. Regulation of Escherichia coli fumarate reductase (frdABCD) operon expression by respiratory electron acceptors and the fnr gene product. J. Bacteriol. 169:3340-3349.
18. Jourlin, C., A. Bengrine, M. Chippaux, and V. Mejean. 1996. An unorthodox sensor protein (TorS) mediates the induction of the Tor structural genes in response to trimethylamine N-oxide in Escherichia coli. Mol. Microbiol. 20:1297-1306.
19. 4 dicarboxylic acids by Escherichia coli. Eur. J. Biochem. 18:274-281.
20. Kolb, A., and S. J. W. Busby. 1996. The Cap Modulon, p. 255-280. In E. C. C. Lin and A. S. Lynch (ed.), Regulation of gene expression in Escherichia coli. Landes Co. and Chapman & Hall, Washington, D.C.
21. Lin, E. C. C. 1996. Dissimilatory pathways for sugars, polyols, and carboxylates, p. 307-342. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaecther, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C.
22. Lo, T. C. Y. 1977. The molecular mechanism of dicarboxylic acid transport in Escherichia coli K12. J. Supramol. Struct. 7:463-480.
23. Lo, T. C. Y., K. Rayman, and B. D. Sanwal. 1972. Transport of succinate in Escherichia coli K12. J. Biol. Chem. 247:6323-6331.
24. McFall, E., and E. B. Newman. 1996. Amino acids as carbon sources, p. 358-379. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C.
25. Meyer, M., P. Dimroth, and M. Bott. 1997. In vitro binding of the response regulator CitB and of its carboxy-terminal domain to A+T-rich DNA target sequences in the control region of the divergent citC and citS operons of Klebsiella pneumoniae. J. Mol. Biol. 269:719-731.
26. Miller, J. H. 1992. A short course in bacterial genetics: a laboratory manual and handbook for E. coli and related bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
27. Phillips-Jones, M. K., F. J. Watson, and R. Martin. 1993. The 3′ codon context effect on UAG suppressor tRNA is different in Escherichia coli and human cells. J. Mol. Biol. 233:1-6.
28. Quail, M. A., D. J. Haydon, and J. R. Guest, 1994. The pdhR-aceEF-lpd operon of Escherichia coli expresses the pyruvate dehydrogenase complex. Mol. Microbiol. 12:95-104.
29. Reizer, L. J. 1996. Ammonia assimilation and the biosynthesis of glutamine, glutamate, aspartate, asparagine, L-alanine, and D-alanine, p. 391-407. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C.
30. Sarabrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. Sawers, G., and B. Suppmann. 1992. Anaerobic induction of pyruvate formate-lyase gene expression is mediated by ArcA and FNR proteins. J. Bacteriol. 174:3474-3478.
32. Scott, S., S. Busby, and I. Beacham. 1995. Transcriptional co-activation at the ansB promoters: involvement of the activating regions of CRP and FNR when bound in tandem. Mol. Microbiol. 18:521-531.
33. Silhavy, T. J., M. L. Barman, and L. W. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
34. Simon, G., V. Mejean, C. Jourlin, M. Chippaux, and M. C. Pascal. 1994. The TorR gene of Escherichia coli encodes a response regulator protein involved in the expression of trimethylamine N-oxide reductase genes. J. Bacteriol. 176:5601-5606.
35. Simons, R. W., F. Houman, and N. Kleckner. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53:85-96.
36. 4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct). J. Bacteriol. 176:6470-6478.
37. Sofia, H. J., V. Burland, D. L. Daniels, G. Plunkett III, and F. R. Blattner. 1994. Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes. Nucleic Acids Res. 22:2576-2586.
38. Spencer, M. E., V. M. Lebeter, and J. R. Guest. 1976. Location of the aspartase gene (aspA) on the linkage map of Escherichia coli K12. J. Gen. Microbiol. 97:73-82.
39. Spiro, S., and J. R. Guest. 1988. Inactivation of the FNR protein of Escherichia coli by targeted mutagenesis in the N-terminal region. Mol. Microbiol. 2:701-707.
40. Staden, R. 1996. The Staden sequence-analysis package. Mol. Biotechnol. 5:233-241.
41. Tseng, C.-P. 1997. Regulation of fumarase (fumB) gene expression in Escherichia coli in response to oxygen, iron and heme availability: role of the arcA, fur, and hemA gene products. FEMS Microbiol. Lett. 157:67-72.
42. Vieira, J., and J. Messing. 1987. Production of single-stranded plasmid DNA. Methods Enzymol. 153:3-11.
43. Woods, S. A., J. S. Miles, R. E. Roberts, and J. R. Guest. 1986. Structural and functional relationships between fumarase and aspartase. Nucleotide sequence of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12. Biochem. J. 237:547-557.
44. Woods, S. A., and J. R. Guest. 1987. Differential roles of the Escherichia coli fumarases and fnr-dependent expression of fumarase B and aspartase. FEMS Microbiol. Lett. 48:219-224.
45. 4-dicarboxylate transport in Escherichia coli: roles of the three Dcu carriers in uptake and exchange. J. Bacteriol. 178: 7241-7247.