Topological analysis of dcuA, an anaerobic C4-dicarboxylate transporter of Escherichia coli

Journal of Bacteriology

Volumn 180, Issue 18, 1998, Pages 4821-4827

  Golby, Paul ^a   Kelly, David J ^b   Guest, John R ^b   Andrews, Simon C ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; DCUA PROTEIN; DICARBOXYLIC ACID; GENE PRODUCT; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL MEMBRANE; CELL MEMBRANE TRANSPORT; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0031656845     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.18.4821-4827.1998     Document Type: Article

References (34)

1. Andrews, S. C. Unpublished data.
2. Baker, K. E., K. P. Ditullio, J. Neuhard, and R. A. Kelln. 1996. Utilization of orotate as a pyrimidine source by Salmonella typhimurium and Escherichia coli requires the dicarboxylate transport protein encoded by dctA. J. Bacteriol. 178:7099-7105.
3. Broome-Smith, J. K., and B. G. Spratt. 1986. A vector for the construction of translational fusions to TEM β-lactamase and the analysis of protein export signals and membrane protein topology. Gene 49:341-349.
4. Calamia, J., and C. Manoil. 1990. lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion. Proc. Natl. Acad. Sci. USA 87:4937-4941.
5. Devereux, J. 1989. The GCG sequence analysis software package, version 6.0 Genetics Computer Group, Inc., Madison, Wis.
6. Eisenberg, D., E. Schwarz, M. Komaromy, and R. Wall. 1984. Analysis of membrane and surface protein sequences with the hydrophobia moment plot. J. Mol. Biol. 179:125-142.
7. Engel, P., R. Krämer, and G. Unden. 1992. Anaerobic fumarate transport in Escherichia coli by an fnr-dependent dicarboxylate uptake system which is different from aerobic dicarboxylate uptake. J. Bacteriol. 174:5533-5539.
8. 4-dicarboxylates by anaerobically grown Escherichia coli: energetics and mechanism of exchange, uptake and efflux. Eur. J. Biochem. 222:605-614.
9. Gribskov, M., R. Luthy, and D. Eisenberg. 1990. Profile analysis. Methods Enzymol. 183:146-159.
10. Griffith, J. K., M. E. Baker, D. A. Rouch, M. G. P. Page, R. A. Skurray, L. Paulsen, K. F. Chater, S. A. Baldwin, and P. J. F. Henderson. 1992. Membrane transport proteins: implications of sequence comparisons. Curr. Opin. Cell Biol. 4:684-695.
11. Guest, J. R., R. E. Roberts, and R. J. Wilde. 1984. Cloning of the aspartase gene (aspA) of Escherichia coli. J. Gen. Microbiol. 130:1271-1278.
12. + symport protein, FucP, for transport of L-fucose into Escherichia coli. Mol. Microbiol. 12:799-809.
13. Henderson, P. J. F. 1993. The 12-transmembrane helix transporters. Curr. Opin. Cell Biol. 5:708-721.
14. 4 dicarboxylic acids by Escherichia coli. Eur. J. Biochem. 18:274-281.
15. Klein, P., M. Kanehisa, and C. DeLisi. 1985. The detection and classification of membrane spanning proteins. Biochim. Biophys. Acta 815:468-476.
16. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
17. 0 assembly. J. Biol. Chem. 267:3482-3489.
18. Lo, T. C. Y. 1977. The molecular mechanism of dicarhoxylic acid transport in Escherichia coli K12. J. Supramol. Struct. 7:463-480.
19. Maloney, P. C. 1990. A consensus structure for membrane proteins. Res. Microbiol. 141:374-383.
20. Moore, K. E., and S. Miura. 1987. A small hydrophobic domain anchors leader peptidase to the cytoplasmic membrane of Escherichia coli. J. Biol. Chem. 262:8806-8813.
21. Osborn, M. J., and R. Munson. 1974. Separation of the inner (cytoplasmic) and outer membranes of gram-negative bacteria. Methods Enzymol. 31:642-653.
22. Peterson, G. L. 1977. A simplification of the protein assay method of Lowry et al. which is more generally acceptable. Anal. Biochem. 83:346-356.
23. Pourcher, T., M. Bassilana, H. K. Sarkar, R. Kaback, and G. Leblanc. 1990. Melibiose permease and α-galactosidase of Escherichia coli: identification by selective labelling using a T7 RNA polymerase/promoter expression system. Biochemistry 29:690-696.
24. Saier, M. H. 1990. Evolution of permease diversity and energy-coupling mechanisms - an introduction. Res. Microbiol. 141:282-286.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Sarsaro, J. P., and A. J. Pittard. 1995. Membrane topology analysis of Escherichia coli K-12 Mtr permease by alkaline phosphatase and β-galactosidase fusions. J. Bacteriol. 177:297-306.
27. 4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the anaerobic dicarboxylate transport system (Dct). J. Bacteriol. 176:6470-6478.
28. Sofia, H. J., V. Burland, D. L. Daniels, G. Plunkett III, and F. R. Blattner. 1994. Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes. Nucleic Acids Res. 22:2576-2586.
29. + transport protein in Salmonella typhimurium and Escherichia coli. J. Biol. Chem. 267:6923-6932.
30. + transport protein (RhaT) from enterobacteria. J. Biol. Chem. 268:26850-26857.
31. Vieira, J., and J. Messing. 1987. Production of single-stranded plasmid DNA. Methods Enzymol. 153:3-11.
32. von Heijne, G. 1992. Membrane protein structure prediction: hydrophobicity analysis and the positive inside rule. J. Mol. Biol. 225:487-494.
33. Zhang, Y., and J. K. Broome-Smith. 1990. Correct insertion of a simple eukaryotic plasma-membrane protein into the cytoplasmic membrane of Escherichia coli. Gene 96:51-57.
34. 4-dicarboxylate transport in Escherichia coli: roles of the three Dcu carriers in uptake and exchange. J. Bacteriol. 178: 7241-7247.