Microscopic and molecular in situ characterization of bacterial populations in the meromictic Lake Cadagno

Documenta dell'Istituto Italiano di Idrobiologia

Volumn , Issue 63, 1998, Pages 31-44

  Tonolla, Mauro ^a   Demarta, Antonella ^a   Hahn, Dittmar ^b   Peduzzi, Raffaele ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b ETH ZURICH   (Switzerland)

Author keywords

Amoebobacter; Chromatium; Fluorescent oligonucleotide probes; In situ hybridization; Phototrophic sulfur bacteria; Sulfate reducing bacteria

Indexed keywords

AEROBIC METABOLISM; AMOEBOBACTER PURPUREUS; ANAEROBIC METABOLISM; CHROMATIUM OKENII; FLUORESCENCE IN SITU HYBRIDIZATION; MICROBIAL FUNCTION; POPULATION STRUCTURE; PROBE;

BACTERIOPLANKTON; LAKE;

SWITZERLAND;

AMOEBOBACTER; CHROMATIUM;

EID: 0032411008     PISSN: 03938395     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Alfreider, A.J. Pernthaler, R. Amann, B. Sattler, F.-O. Glöckner, A. Wille & R. Psenner. 1996. Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization. Appl. Environ. Microbiol., 62: 2138-2144.
2. Amann, R.I., L. Krumholz & D.A. Stahl. 1990a. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol., 172: 762-770.
3. Amann, R.I., B.J. Binder, R.J. Olson, S.W. Chisholm, R. Devereux & D.A. Stahl. 1990b Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol., 56: 1919-1925.
4. Amann, R., W. Ludwig & K.-H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev., 59: 143-169.
5. Bensadoun, J-C., M. Tonolla, A. Demarta, F. Barja & R. Peduzzi. 1998. Vertical distribution and microscopic characterization of a non-culturable microorganism (morphotype R) of the Lake Cadagno. In: Peduzzi, R., R. Bachofen & M. Tonolla (Eds), Lake Cadagno: a meromictic Alpine lake. Documenta Ist. ital. Idrobiol., 63: 45-51.
6. Caldwell, D.E. & J.M. Tiedje. 1975. The structure of anaerobic bacterial communities in the hypolimnia of several Michigan lakes. Can. J. Microbiol., 21: 377-385.
7. Clark, A.E. & E. Walsby. 1978. The development and vertical distribution of populations of gas-vacuole bacteria in a eutrophic, monomictic lake. Arch. Microbiol., 118: 229-233.
8. Eichler, B. & N. Pfennig. 1988. A new purple sulfur bacterium from stratified freshwater lakes, Amoebobacter purpureus sp. nov. Arch. Microbiol., 149: 395-400.
9. Fischer, K., D. Hahn, R.I. Amann, O. Daniel & J. Zeyer. 1995. In situ analysis of the bacterial community in the gut of the earthworm Lumbricus terrestris L. by whole-cell hybridization. Can. J. Microbiol., 41: 666-673.
10. Fischer, C., M. Wiggli, F. Schanz, K.W. Hanselmann & R. Bachofen. 1996. Light environment and synthesis of bacteriochlorophyll by populations of Chromatium okenii under natural environmental conditions. FEMS Microbiol. Ecol., 21: 1-9.
11. Glöckner, F.-O., R. Amann, A. Alfreider, J. Pernthaler, R. Psenner, K. Trebesius & K.-H. Schleifer. 1996. An in situ hybridization protocol for detection and identification of planktonic bacteria. System. Appl. Microbiol., 19: 403-406.
12. Guerrero, R., C. Abella & M.R. Miracle. 1978. Spatial and temporal distribution of bacteria in a meromictic karsic lake basin; relationships with physicochemical parameters and zooplankton. Verh. int. Ver. Limnol., 29: 2264-2271.
13. Guerrero, R., E. Montesinos, C. Pedros-Alio, I. Esteve, J. Mas, H. van Gemerden, P.A.G. Hofman & J.F. Bakker. 1985. Phototrophic sulfur bacteria in two Spanish Lakes: vertical distribution and limiting factors. Limnol. Oceanogr., 30: 919-931.
14. Hicks, R.E., R.I. Amann & D.A. Stahl. 1992. Dual staining of natural bacterioplankton with 4′,6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences. Appl. Environ. Microbiol., 58: 2158-2163.
15. Hobbie, J.E., R.J. Daley & S. Jasper. 1977. Use of nucleopore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol., 33: 1225-1228.
16. Jørgensen, B.B., J.G. Kuenen & Y. Cohen. 1979. Microbial tansformations of sulfur compounds in a stratified lake (Solar Lake, Sinai). Limnol. Oceanogr., 24: 799-822.
17. Overmann, J., J.T. Beatty, H.R. Krouse & K.J. Hall. 1996. The sulfur cycle in the chemocline of a meromictic salt lake. Limnol. Oceanogr., 41: 147-156.
18. Overmann, J., J.T. Beatty, K.J. Hall, N. Pfennig & T.G. Northcote. 1991. Characterization of a dense, purple sulfur bacterial layer in a meromictic lake. Limnol. Oceanogr., 36: 846-859.
19. Pedros-Alio, C., E. Montesinos & R. Guerrero. 1983. Factors determining annual changes in bacterial photosynthetic pigments in holomictic Lake Ciso, Spain. Appl. Environ. Microbiol., 46: 999-1006.
20. Peduzzi, R., A. Demarta & M. Tonolla. 1993. Dynamics of the autochthonous and contaminant bacterial colonization of lakes (Lake of Cadagno and Lake of Lugano as model systems), pp. 323-335. In: Vernet, J.-P. (Ed.), Studies in Environmental Science. 55. Environmental Contamination. Elsevier Publ., Amsterdam, The Netherlands.
21. Porter, K.G. & Y.S. Feig. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr., 25: 943-948.
22. Pringault, O., R. de Wit & P. Caumette. 1996. A benthic gradient chamber for culturing phototrophic sulfur bacteria on reconstituted sediments. FEMS Microbiol. Ecol., 20: 237-250
23. Ramsing, N.B., H. Fossing, T.G. Felderman, F. Andersen & B. Thamdrup. 1996. Distribution of bacterial populations in a stratified fjord (Mariager Fjord, Denmark) quantified by in situ hybridization and related chemical gradients in water column. Appl. Environ. Microbiol., 62: 1391-1404.
24. Sorokin, Y.I. 1970. Interrelations between sulphur and carbon turnover in meromictic lakes. Arch. Hydrobiol., 66: 391-446.
25. Wagener, S., S. Schulz & K. Hanselmann. 1990. Abundance and distribution of anaerobic protozoa and their contribution to methane production in Lake Cadagno (Switzerland). FEMS Microbiol. Ecol., 74: 39-48.
26. Wallner, G., B. Fuchs, S. Spring, W. Beisker & R. Amann. 1997. Flow sorting of microorganisms for molecular analysis. Appl. Environ. Microbiol., 63: 4223-4231.
27. Weiss, P., B. Schweitzer, R. Amann & M. Simon. 1996. Identification in situ and dynamics of bacteria on limnetic organic aggregates (lake snow). Appl. Environ. Microbiol., 62: 1998-2005.
28. Zarda, B., D. Hahn, A. Chatzinotas, W. Schönhuber, A. Neef, R.I. Amann & J. Zeyer. 1997. Analysis of bacterial community structure in bulk soil by in situ hybridization. Arch. Microbiol., 168: 185-192.