Alfalfa (Medicago sativa L.) resistance to the root-lesion nematode, Pratylenchus penetrans: Defense-response gene mRNA and isoflavonoid phytoalexin levels in roots

Plant Molecular Biology

Volumn 38, Issue 6, 1998, Pages 999-1010

  Baldridge, Gerald D ^a   O'Neill, Nichole R ^b   Samac, Deborah A ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b Soybean and Alfalfa Res Laboratory   (United States)

Author keywords

Chitinase; Disease resistance; Glucanase; Isoflavonoids; mRNA; Phytoalexins

Indexed keywords

1,3 BETA GLUCANASE; BIOCHEMICAL PATHWAY; BIOSYNTHESIS; CAFFEIC ACID O METHYLTRANSFERASE; CHALCONE SYNTHASE; CHITINASE; DISEASE RESISTANCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOST RESISTANCE; ISOFLAVONE REDUCTASE; MEDICARPIN; MESSENGER RNA; METABOLITE; PHENYLALANINE AMMONIA LYASE; PHENYLPROPANOID; PLANT GENETICS; PLANT PATHOGEN INTERACTION; PLANT VARIETY; RECURRENT SELECTION; RNA ANALYSIS;

ACYLTRANSFERASES; ANIMALS; BETA-GLUCOSIDASE; CHITINASE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GLUCAN 1,3-BETA-GLUCOSIDASE; IMMUNITY, NATURAL; ISOFLAVONES; MEDICAGO SATIVA; METHYLTRANSFERASES; NEMATODA; OXIDOREDUCTASES; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; PHENYLALANINE AMMONIA-LYASE; PLANT EXTRACTS; PLANT ROOTS; RNA, MESSENGER; TERPENES; TRANSCRIPTION, GENETIC;

EMBRYOPHYTA; MEDICAGO SATIVA; PRATYLENCHUS PENETRANS;

EID: 0032406089     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006182908528     Document Type: Article

References (62)

1. Barnes DK, Thies JA, Rabas DL, Nelson DL, Smith DM: Registration of two alfalfa germplasms with field resistance to the root lesion nematode. Crop Sci 30: 751-752 (1990).
2. Basigalup DH: Resistance to Pratylenchus penetrans in alfalfa: mode of inheritance and effects on biological nitrogen fixation. M.S. thesis, Dept. of Agronomy, University of Minnesota (1990).
3. Bird AF, Bird J: The Structure of Nematodes, 2nd ed., pp. 44-74. Academic Press, San Diego, CA (1991).
4. Blount JW, Dixon RA, Paiva NL: Stress responses in alfalfa (Medicago sativa L.) XVI. Antifungal activity of medicarpin and its biosynthetic precursors; implications for the genetic manipulation of stress metabolites. Physiol Mol Plant Path 141: 333-349 (1992).
5. Brosch G, Ransom R, Lechner T, Walton JD, Loidl P: Inhibition of maize deacetylases by HC toxin, the host-selective toxin of Cochliobolus carbonum. Plant Cell 7: 1941-1950 (1995).
6. Brueske CH: Phenylalanine ammonia lyase activity in tomato roots infected and resistant to the root-knot nematode, Meloidogyne incognita. Physiol Plant Path 16: 409-414 (1980).
7. Cai, D, Kleine M, Kifle S, Harloff H-J, Sandal NN, Marcker KA, Klein-Lankhorst RM, Salentijn EMJ, Lange W, Stiekma WJ, Wyss U, Grundler FMW, Jung C: Positional cloning of a gene for nematode resistance in sugar beet. Science 275: 832-834 (1997).
8. Chang LM: The repellent effect of necrotic tissues on the nematode Pratylenchus penetrans (Cobb, 1917), Filipjev and Schurmans Stekhoven, 1941. M.S. thesis, University of Massachusetts (1969).
9. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium isothyiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159 (1987).
10. Davis KR, Darvill AG, Albersheim P: Several biotic and abiotic elicitors act synergistically in the induction of phytoalexin accumulation in soybean. Plant Mol Biol 6: 23-32 (1986).
11. Ebel J, Cosio EG: Elicitors of plant defense responses. Int Rev Cytol 148: 1-31 (1994).
12. Edens RM, Anand SC, Bolla RI: Enzymes of phenylpropanoid pathway in soybean infected with Meloidogyne incognita or Heterodera glycines. J Nematol 27: 292-303 (1995).
13. Edwards R, Mizen KA, Cook R: Isoflavonoid conjugate accumulation in the roots of lucerne (Medicago sativa) seedlings following infection by the stem nematode (Ditylenchus dispaci). Nematologica 41: 51-66 (1995).
14. 32P-based hybridizations. Anal Biochem 210: 235-244 (1993).
15. Forrest JMS, Robertson WM: Characterization and localization of saccharides on the head region of four populations of the potato cyst nematode Globodera rostochiensis and G. pallida. J Nematol 18: 23-26 (1986).
16. Gamborg OL, Miller RA, Ojima K: Nutritional requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158 (1968).
17. Goddijn OJM, Lindsey K, van der Lee FM, Klap JC, Sijmons PC: Differential gene expression in nematode-induced feeding structures of transgenic plants harbouring promoter-gusA fusion constructs. Plant J 4: 863-873 (1993).
18. Gowri G, Paiva NL, Dixon RA: Stress responses in alfalfa (Medicago sativa L.) 12. Sequence analysis of phenylalanine ammonia-lyase (PAL) cDNA clones and appearance of PAL transcripts in elicitor-treated cell cultures and developing plants. Plant Mol Biol 17: 415-429 (1991).
19. Gowri G, Bugos RC, Campbell WH, Maxwell CA, Dixon RA: Stress responses in alfalfa (Medicago sativa L.) X. Molecular cloning and expression of S-adenosyl-L-Methionine:caffeic acid 3-O-methyltransferase, a key enzyme of lignin biosynthesis. Plant Physiol 97: 7-14 (1991).
20. Gurr SJ, McPherson MJ, Scollan C, Atkinson HJ, Bowles DJ: Gene expression in nematode-infected plant roots. Mol Gen Genet 226: 361-366 (1991).
21. Huang JS, Barker KR: Glyceollin I in soybean-cyst nematode interactions. Plant Physiol 96: 1302-1307 (1991).
22. Joosten MHAJ, de Wit PJGM: Identification of several pathogenesis-related proteins in tomato leaves inoculated with Cladosporium fulvum (syn. Fulvia fulva) as 1,3-β-glucanases and chitinases. Plant Physiol 89: 945-951 (1989).
23. Junghans H, Dalkin K, Dixon RA: Stress responses in alfalfa (Medicago saliva L.) 15. Characterization and expression patterns of members of a subset of the chalcone synthase multigene family. Plant Mol Biol 22: 239-253 (1993).
24. Kaplan DT, Keen NT: Mechanisms conferring plant incompatibility to nematodes. Revue Nematol 3: 123-134 (1980).
25. Kaplan DT, Keen NT, Thomason IJ: Association of glyceollin with the incompatible response of soybean roots to Meloidogyne incognita. Physiol Plant Pathol 16: 309-318 (1980).
26. Kaplan DT, Keen NT, Thomason IJ: Studies on the mode of action of glyceollin in soybean incompatibility to the root knot nematode, Meloidogyne incognita. Physiol Plant Path 16: 319-325 (1980).
27. Kapros T, Bogre L, Nemeth K, Bako L, Gyorgyey J, Wu SC, Dudits D: Differential expression of histone H3 variants during cell cycle and somatic embryogenesis. Plant Physiol 98: 621-625 (1991).
28. Kessmann H, Edwards R, Geno PW, Dixon RA: Stress responses in alfalfa (Medicago saliva L) V. Constitutive and elicitor-induced accumulation of isoflavonoid conjugates in cell suspension cultures. Plant Physiol 94: 227-232 (1990).
29. Lambert K, Williamson V: cDNA library construction using PCR. Soc Nematol Mol Biol Newsl 4: 10-11 (1992).
30. Leath KT, Erwin DC, Griffin GD: Diseases and nematodes. In: Hansen AA, Barnes DK, Hill Jr RR (eds) Alfalfa and Alfalfa Improvement. American Society of Agronomy, Madison, WI,. Monograph 29, pp. 621-670 (1988).
31. Maher EA, Lamb CJ, Dixon RA: Stress responses in alfalfa (Medicago sativa L.) XVII. Identification of multiple hydrolases and molecular characterization of an acidic glucanase. Physiol Mol Plant Path 43: 329-342 (1993).
32. Mauch F, Mauch-Mani B, Boller T: Antifungal hydrolases in pea tissue II. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiol 88: 936-942 (1988).
33. Miller KJ, Hadley JA, Gustine FL: Cyclic β-1,6-1,3-glucans of Bradyrhizobium japonicum USDA 110 elicit isoflavonoid production in the soybean (Glycine max) host. Plant Physiol 104: 917-923 (1994).
34. Mittler R, Lam E: Sacrifice in the face of foes: Pathogen-induced programmed cell death in plants. Trends Microbiol 10: 10-15 (1996).
35. Munch S: Nonradioactive northern blots in 1.5 days and with substantially increased sensitivity through 'alkaline blotting'. Biochemica 11: 29-31 (1994).
36. Niebel A, De Almeida Engler J, Tire C, Engler G, Van Montagu M, Gheysen G: Induction patterns of an extensin gene in tobacco upon nematode infection. Plant Cell 5: 1697-1710 (1993).
37. Niebel A, Gheysen G, Van Montagu M: Plant-cyst nematode and plant-root-knot nematode interactions. Parasitol Today 10: 424-430 (1994).
38. O'Neill NR: Defense expression in protected tissues of Medicago sativa is enhanced during compatible interactions with Collelotrichum trifolii. Phytopathology 86: 1045-1050 (1996).
39. Opperman CH, Taylor CG, Conkling MA: Root-knot nematode-directed expression of a plant root-specific gene. Science 263: 221-223 (1994).
40. Painter RH: The mechanisms of resistance. In Insect Resistance in Crop Plants, pp. 23-83. MacMillan, New York (1951).
41. Paiva NL, Edwards R, Sun Y, Hrazdina G, Dixon RA: Stress responses in alfalfa (Medicago saliva L.). 11. Molecular cloning and expression of isoflavone reductase, a key enzyme of isoflavonoid phytoalexin biosynthesis. Plant Mol Biol 17: 653-667 (1991).
42. Potenza CL, Thomas SH, Higgins EA, Sengupta-Gopalan C: Early root response to Meloidogyne incognita in resistant and susceptible alfalfa cultivars. J Nematol 28: 475-484 (1996).
43. Rahimi S, Perry RN, Wright DJ: Identification of pathogenesis-related proteins induced in leaves of potato plants infected with potato cyst nematodes, Globodera species. Physiol Mol Plant Path 49: 49-59 (1996).
44. Rich JR, Keen NT, Thomason: Association of coumestans with the hypersensitivity of lima bean roots to Pratylenchus scribneri. Physiol Plant Path 10: 105-116 (1977).
45. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Plainview, NY (1989).
46. Sela-Buurlage MB, Ponstein As, Bres-Vloemans SA, Melchers LS, van den Elzen PJM, Cornelissen BJC: Only specific tobacco (Nicotiana tabacum) chitinases and β-1,3-glucanases exhibit antifungal activity. Plant Physiol 101: 857-863 (1993).
47. Sijmons PC: Plant-nematode interactions. Plant Mol Biol 23: 17-931 (1993).
48. Smith CJ: Accumulation of phytoalexins: defense mechanism and stimulus response. New Phytol 132: 1-45 (1996).
49. Spanakis E: Problems related to the interpretation of autoradiographic data on gene expression using common constitutive transcripts as controls. Nucl Acids Res 21: 3809-3819 (1993).
50. Spiegel Y, Cohn E, Spiegel S: Characterization of sialyl and galactosyl residues on the body wall of different plant parasitic nematodes. J Nematol 14: 33-39 (1982).
51. Stevenson PC, Turner HC, Haware MP: Phytoalexin accumulation in the roots of chickpea (Cicer arietinum L.) seedlings associated with resistance of fusarium wilt (Fusarium oxysporum f.sp. ciceri). Physiol Mol Plant Path 50: 167-178 (1997).
52. Thies JA, Basigalup D, Barnes DK: Inheritance of resistance to Pratylenchus penetrans in alfalfa. J Nematol 26: 452-459 (1994).
53. Thies JA, Petersen AD, Barnes DK: Host suitability of forage grasses and legumes for root-lesion nematode Pratylenchus penetrans. Crop Sci 35: 1647-1651 (1995).
54. Tiller SA, Parry AD Edwards R: Changes in the accumulation of flavonoid and isoflavonoid conjugates associated with plant age and nodulation in alfalfa (Medicago sativa). Physiol Plant 91: 27-36 (1994).
55. Townshend JL, Stobbs L, Carter R: Ultrastructural pathology of cells affected by Praylenchus penetrans in alfalfa roots. J Nematol 21: 530-539 (1989).
56. Townshend JL, Stobbs L: Histopathology and histochemistry of lesions caused by Pratylenchus penetrans in roots of forage legumes. Can J Plant Path 3: 123-186 (1981).
57. Trudgill DL: Resistance to and tolerance of plant parasitic nematodes. Annu Rev Phytopath 29: 167-192 (1991).
58. Van der Eycken W, Engler J, Inzé D, Van Montagu M, Gheysen G: A molecular study of root-knot nematode-induced feeding sites. Plant J 9: 45-54 (1996).
59. Wu SC, Grorgyey J, Dudits D: Polyadenylated H3 histone transcripts and H3 histone variants in alfalfa. Nucl Acids Res 17: 3057-3063 (1989).
60. Wyss U: Ectoparasitic root nematodes: Feeding behavior and plant cell responses. In: Zuckerman BM, Rohde RA (eds) Plant Parasitic Nematodes, pp. 211-220. Academic Press, New York (1981).
61. Zacheo G, Bleve-Zacheo T, Lamberti F: Role of peroxide and Superoxide dismutase in resistant and susceptible tomato cultivars infested by Meloidogyne incognita. Nematol Mediterr 10: 75-80 (1982).
62. Zunke, U: Observations on the invasion and endoparasitic behavior of the root lesion nematode Pratylenchus penetrans. J Nematol 22: 309-320 (1990).