Detection of microorganisms in vessel wall specimens of the abdominal aorta: Development of a PCR assay in the absence of a gold standard

Research in Microbiology

Volumn 149, Issue 8, 1998, Pages 577-583

  Meijer, A ^a   Van Der Vliet, J A ^b   Schouls, L M ^a   De Vries, A ^a   Roholl, P J M ^a   Ossewaarde, J M ^a  

^a NATIONAL INSTITUTE FOR PUBLIC HEALTH AND THE ENVIRONMENT   (Netherlands)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Atherosclerosis; Chlamydia pneumoniae; Cytomegalovirus; DNA; Herpes simplex virus; PCR; Tissue detection

Indexed keywords

BACTERIAL DNA; VIRUS DNA;

ABDOMINAL AORTA; ACCURACY; ARTICLE; BACTERIUM DETECTION; CHLAMYDOPHILA PNEUMONIAE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA DETERMINATION; HERPES SIMPLEX VIRUS 1; HERPES SIMPLEX VIRUS 2; HUMAN; HUMAN TISSUE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STANDARD; VALIDATION PROCESS; VIRUS DETECTION;

EID: 0032405999     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(99)80005-9     Document Type: Article

References (15)

1. Blasi, F., Denti, F., Erba, M., Cosentini, R., Raccanelli, R., Rinaldi, A., Fagetti, L., Esposito, G., Ruberti, U. & Allegra, L. (1996), Detection of Chlamydia pneumoniae but not Helicobacter pylori in atherosclerotic plaques of aortic aneurysms. J. Clin. Microbiol, 34, 2766-2769.
2. Boom, R., Sol, C.J.A., Salimans, M.M.M., Jansen, C.L., Wertheim-van Dillen, P.M.E. & van der Noordaa, J. (1990), Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol, 28, 495-503.
3. Campbell, L.A., O'Brien, E.R., Cappuccio, A.L., Kuo, C.-C., Wang, S.-P., Stewart, D. Patton, D.L., Cummings, P.K. & Grayston, J.T. (1995), Detection of Chlamydia pneumoniae TWAR in human coronary atherectomy tissues. J. Infect. Dis., 172, 585-588.
4. Chou, Q., Russell, M., Birch, D.E., Raymond, J. & Bloch, W. (1992), Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res., 20, 1717-1723.
5. Clewley, J.P. (1993), PCR without tears. PHLS Microbiol. Digest, 10, 169-174.
6. Danesh, J., Collins, R. & Peto R. (1997), Chronic infections and coronary heart disease : is there a link? Lancet, 350, 430-436.
7. de Francis, R., Cross, N.C.P., Foulkes, N.S. & Cox, T.M. (1988), A potent inhibitor of Taq polymerase copurifies with human genomic DNA. Nucleic Acids Res., 16, 10355.
8. Don, R.H., Cox, P.T., Wainwright, B.J., Baker, K. & Mattick, J.S. (1991), 'Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res., 19, 4008.
9. Fredericks, D.N. & Relman, D.A. (1996), Sequence-based identification of microbial pathogens : a reconsideration of Koch's postulates. Clin. Microbiol. Rev., 9, 18-33.
10. Hung, T., Mak, K. & Fong, K. (1990), A specificity enhancer for polymerase chain reaction. Nucleic Acids Res., 18, 4953.
11. Kuo, C-C., Shor, A., Campbell, L.A., Fukushi, H., Patton, D.L. & Grayston, J.T. (1993), Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries. J. Infect. Dis., 167, 841-849.
12. Lo, Y.-M.D. (1994), Detection of minority nucleic acid populations by PCR. J. Pathol., 174, 1-6.
13. Meijer, A., Kwakkel, G.J., de Vries, A., Schouls, L.M. & Ossewaarde, J.M. (1997), Species identification of Chlamydia isolates by analyzing restriction fragment length polymorphism of the 16S-23S rRNA spacer region. J. Clin. Microbiol., 35, 1179-1183.
14. Ramirez, J.A. and the Chlamydia pneumoniae/Atherosclerosis Study Group (1996), Isolation of Chlamydia pneumoniae from the coronary artery of a patient with coronary atherosclerosis. Ann. Intern. Med., 125, 979-982.
15. Weiss, S.M., Roblin, P.M., Gaydos, C.A., Cummings, P., Patton, D.L., Schulhoff, N., Shani, J., Frankel, R., Penney, K., Quinn, T.C., Hammerschlag, M.R. & Schachter, J. (1996), Failure to detect Chlamydia pneumoniae in coronary atheromas of patients undergoing atherectomy. J. Infect. Dis., 173, 957-962.