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1
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0031453850
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Genome wide expression monitoring in Saccharomyces cerevisiae
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of outstanding interest. Oligonucleotide arrays are used to measure the expression levels for all predicted S. cerevisiae genes. High sensitivity and good correlation with well studied mRNA changes are reported. Use of single base mismatched oligonucleotides for specificity control is described.
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Wodicka L, Dong H, Mittmann M, Ho MH, Lockhart DJ. Genome wide expression monitoring in Saccharomyces cerevisiae. of outstanding interest Nat Biotechnol. 15:1997;1359-1367 Oligonucleotide arrays are used to measure the expression levels for all predicted S. cerevisiae genes. High sensitivity and good correlation with well studied mRNA changes are reported. Use of single base mismatched oligonucleotides for specificity control is described.
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Nat Biotechnol
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Wodicka, L.1
Dong, H.2
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2
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0030669030
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Exploring the metabolic and genetic control of gene expression on a genomic scale
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of outstanding interest. Microarrays of virtually the entire S. cerevisiae genome were created using gene-specific primers to amplify genes as targets for the microarrays. These were then used to analyze the gene expression changes accompanying the shift from fermentation to respiration. Known changes were observed but changes in unknown genes gave indications as to their biological function.
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DeRisi JL, Iyer VR, Brown PO. Exploring the metabolic and genetic control of gene expression on a genomic scale. of outstanding interest Science. 278:1997;680-686 Microarrays of virtually the entire S. cerevisiae genome were created using gene-specific primers to amplify genes as targets for the microarrays. These were then used to analyze the gene expression changes accompanying the shift from fermentation to respiration. Known changes were observed but changes in unknown genes gave indications as to their biological function.
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Derisi, J.L.1
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Discovery and anlysis of inflammatory disease-related genes using cDNA microarrays
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of special interest. The expression levels of 96 probable inflammatory-related genes are studied in several different tissue specimens showing the potential use of microarray technology in targeted experiments.
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Heller RA, Schena M, Chai A, Shalon D, Bedilion T, Gilmore J, Woolley DE, Davis RW. Discovery and anlysis of inflammatory disease-related genes using cDNA microarrays. of special interest Proc Natl Acad Sci USA. 94:1997;2150-2155 The expression levels of 96 probable inflammatory-related genes are studied in several different tissue specimens showing the potential use of microarray technology in targeted experiments.
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Proc Natl Acad Sci USA
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Heller, R.A.1
Schena, M.2
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Guo Z, Guilfoyle RA, Thiel AJ, Wang R, Smith LM. Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports. Nucleic Acids Res. 22:1994;5456-5465.
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15
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0031553975
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Covalent attachment of hybridizable oligonucleotides to glass supports
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of special interest. A straightforward and robust method for covalent attachment of oligonucleotides to glass is presented. This chemistry is well suited to the creation of high density printed arrays of oligonucleotides.
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Joos B, Kuster H, Cone R. Covalent attachment of hybridizable oligonucleotides to glass supports. of special interest Anal Biochem. 247:1997;96-101 A straightforward and robust method for covalent attachment of oligonucleotides to glass is presented. This chemistry is well suited to the creation of high density printed arrays of oligonucleotides.
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Joos, B.1
Kuster, H.2
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0030862620
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of outstanding interest. This paper quantitatively examines the effects of spacer molecules to reduce steric hindrance of the support on the hybridization behavior of immobilized oligonucleotides. Interestingly, steric hindrance is also a problem if the oligonucleotides attached to the support are too close to each other, with the optimum being a surface containing around 50% of the maximum concentration of oligonucleotides.
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Shchepinov MS, Case-Green SC, Southern EM. Steric factors influencing hybridization of nucleic acids to oligonucleotide arrays. of outstanding interest Nucleic Acids Res. 25:1997;1155-1161 This paper quantitatively examines the effects of spacer molecules to reduce steric hindrance of the support on the hybridization behavior of immobilized oligonucleotides. Interestingly, steric hindrance is also a problem if the oligonucleotides attached to the support are too close to each other, with the optimum being a surface containing around 50% of the maximum concentration of oligonucleotides.
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Shchepinov, M.S.1
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0032526318
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Detection of differentially expressed genes in primary tumor tissues using representational differences analysis coupled to microarray hybridization
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of special interest. A cDNA library subtraction is performed in order to enrich for cDNA clones that are differentially expressed. These clones are then used as the microarray targets.
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Welford SM, Gregg J, Chen E, Garrison D, Sorensen PH, Denny CT, Nelson SF. Detection of differentially expressed genes in primary tumor tissues using representational differences analysis coupled to microarray hybridization. of special interest Nucleic Acids Res. 26:1998;3059-3065 A cDNA library subtraction is performed in order to enrich for cDNA clones that are differentially expressed. These clones are then used as the microarray targets.
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Nucleic Acids Res
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Welford, S.M.1
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Hilier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res. 6:1996;807-828.
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of special interest. A description of high-throughput oligonucleotide synthesis applied to the production of PCR primers for the amplification of coding DNA from genomic DNA. A high first time success rate in PCR amplification is achieved.
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Lashkari DA, McCusker JH, Davis RW. Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR. of special interest Proc Natl Acad Sci USA. 94:1997;8945-8947 A description of high-throughput oligonucleotide synthesis applied to the production of PCR primers for the amplification of coding DNA from genomic DNA. A high first time success rate in PCR amplification is achieved.
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Lashkari, D.A.1
McCusker, J.H.2
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Microarrays: Biotechnology's discovery platform for functional genomics
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of outstanding interest. This review paper contains a good overview of the use of microarrays and describes the results of using noncontact printing for array synthesis
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Schena M, Heller R, Theriault T, Konrad K, Lachenmeier E, Davis R. Microarrays: biotechnology's discovery platform for functional genomics. of outstanding interest Trends Biotechnol. 16:1998;301-306 This review paper contains a good overview of the use of microarrays and describes the results of using noncontact printing for array synthesis.
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Trends Biotechnol
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Schena, M.1
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The McGuide on World Wide Web URL: ttp://cmgm.stanford.edu/pbrown/mguide/index.html of special interest
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The McGuide on World Wide Web URL: ttp://cmgm.stanford.edu/pbrown/mguide/index.html of special interest.
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24
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Instructions for constructing the Stanford array printer, used in many of the cDNA printing applications, are provided.
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Instructions for constructing the Stanford array printer, used in many of the cDNA printing applications, are provided.
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25
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Adapting the biomek 2000 laboratory automation workstation for printing DNA microarrays
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of special interest. A method for the creation and analysis of arrays with thousands of micrometer sized elements is described. Mixtures of microspheres, each a different assay, are applied to the array.
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